Trabecular bone remodelling in the femur of C57BL/6J mice treated with diclofenac in combination with treadmill exercise

• Autorzy: Lehmann Tomasz P. , Wojtków Magdalena , Pruszyńska-Oszmałek Ewa , Kołodziejski Paweł , Pezowicz Celina , Trzaskowska Aleksandra , Mielcarek Sławomir , Szybowicz Mirosław , Nowicka Ariadna B. , Nowicki Marek , Misterska Ewa , Iwańczyk-Skalska Ewa , Jagodziński Paweł , Głowacki Maciej

Identyfikatory

DOI

10.37190/ABB-01851-2021-01

• Języki publikacji: EN

Abstrakty

EN

Purpose: Analgesic treatment with diclofenac deteriorates bone structure and decreases biomechanical properties. This bone loss has been though to be reversed by training. The impact of exercise on bone treated with diclofenac (DF) has reminded elusive. In the present study, we assayed the combined impact of exercises and DF on mouse femur. Methods: The femur samples we obtained from 30 days treated C57BL/6J female mice. The training group ran on a horizontal treadmill at 12 m/min by 30 min a day (5% grade/slope). The group of ten mice treated with DF received the drug subcutaneously every day (5 mg/kg of body weight/day). The combined group ran on the treadmill and obtained DF. After 30 days, we sacrificed mice and studied their femurs using microcomputed tomography (µCT), dynamic mechanical analysis (DMA) and nanoindentation. Results: We observed that treadmill running and DF decreased trabecular bone volume and mineral density. Combined effect of training and DF was not additive. A significant interaction of both parameters suggested protective effect of training on bone loss provoked by DF. The femur cortical bone shell remained untouched by the training and treatment. The training and the DF treatment did not alter the storage modulus E’ significantly. The unchanged storage modulus would be suggesting on the unaltered bone strength. Conclusions: We concluded that even relatively short time of training with concomitant DF treatment could be protective on trabecular bone. Although viscoelastic properties of the entire femur were not modulated, femur trabecular tissue was thinned by treatment with DF and protected by training.

Słowa kluczowe

EN

storage modulus; treadmill; femur; dynamic mechanical analysis; anti-inflammatory drugs; diclofenac

PL

moduł zachowawczy; bieżnia; kość udowa; dynamiczna analiza mechaniczna; leki przeciwzapalne; diklofenak

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2021
• Tom: Vol. 23, no. 3
• Strony: 3--11
• Opis fizyczny: Bibliogr. 32 poz., rys., tab.

Twórcy

autor

Lehmann Tomasz P.

• Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznań, Poland

autor

Wojtków Magdalena

• Department of Mechanics, Materials and Biomedical Engineering, Wrocław University of Science and Technology, Wrocław, Poland

autor

Pruszyńska-Oszmałek Ewa

• Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Poznań, Poland

autor

Kołodziejski Paweł

• Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Poznań, Poland

autor

Pezowicz Celina

• Department of Mechanics, Materials and Biomedical Engineering, Wrocław University of Science and Technology, Wrocław, Poland

autor

Trzaskowska Aleksandra

• Crystal Physics Division, Faculty of Physics, Adam Mickiewicz University, Poznań, Poland

autor

Mielcarek Sławomir

• Crystal Physics Division, Faculty of Physics, Adam Mickiewicz University, Poznań, Poland

autor

Szybowicz Mirosław

• Institute of Materials Research and Quantum Engineering, Faculty of Technical Physics, Poznan University of Technology, Poznań, Poland

autor

Nowicka Ariadna B.

• Institute of Materials Research and Quantum Engineering, Faculty of Technical Physics, Poznan University of Technology, Poznań, Poland

autor

Nowicki Marek

• Centre for Advanced Technologies, Adam Mickiewicz University, Poznań, Poland

autor

Misterska Ewa

• Department of Pedagogy and Psychology, University of Security, Poznań, Poland

autor

Iwańczyk-Skalska Ewa

• Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznań, Poland

autor

Jagodziński Paweł

• Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznań, Poland

autor

Głowacki Maciej

• Department of Paediatric Orthopaedics and Traumatology, Poznan University of Medical Sciences, Poznań, Poland

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2024).