Simultaneous identification and quantitative determination of azithromycin, clarithromycin, roxithromycin, spiramycin and troleandomycin by thin-layer chromatography and densitometry

• Autorzy: Kwiecień A. , Krzek J. , Gądek M.
• Języki publikacji: EN

Abstrakty

EN

The purpose of this work was to develop a TLC-densitometric method for simultaneous identification and quantitative determination of azithromycin, clarithromycin, roxithromycin, spiramycin, and troleandomycin. The method was developed on TLC aluminium plates precoated with silica gel F254 using solvent system izopropanol:n-hexane:ammonia 25% (8:12:3, v/v/v), which gives compact spots for azithromycin (RF = 0.65), clarithromycin (RF = 0.54), roxithromycin (RF = 0.49), spiramycin (RF = 0.22), and troleandomycin (RF = 0.36). Densitometric analysis was carried out at 478 nm after spraying with (1:4, v/v) sulphuric acid:ethanol and heating at 100°C for 5 min. The linear regression analysis data for the calibration plots showed good linear relationship with correlation coefficient higher than 0.99. The method is distinguished by high sensitivity, with limit of detection (LOD) from 0.34 μg/spot for troleandomycin to 0.67 μg/spot for clarithromycin and limit of quantification (LOQ) from 1.02 μg/spot for troleandomycin to 2.04 μg/spot for clarithromycin and a wide linearity range from 2 to 12 μg/spot for spiramycin and 2–15 μg/spot for other antibiotics. The precision of the determination was good; relative standard deviation (RSD) varied in the range from 1.49% to 4.14%.

Słowa kluczowe

EN

macrolide antibiotics; pharmaceutical research; method validation; thin-layer chromatography; densitometry

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 4
• Strony: 657--670
• Opis fizyczny: Bibliogr. 32 poz., rys., tab.

Twórcy

autor

Kwiecień A.

• Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland

autor

Krzek J.

• Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland

autor

Gądek M.

• Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland

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