Simultaneous determination of nine flavonoids in beagle dog by HPLC with DAD and application of Ginkgo biloba extracts on the pharmacokinetic

• Autorzy: Wu J. , Xing H , Tang D. , Gao Y. , Yin X , Du Q. , Jiang X. , Yang D.
• Języki publikacji: EN

Abstrakty

EN

The method of high-performance liquid chromatography (HPLC) with diode array detector (DAD) was used and validated for the simultaneous determination of nine flavonoids (rutin, myricetin, quercitrin, quercetin, luteolin, genistein, kaempferol, apigenin, and isorhamnetin) in beagle dog plasma. Plasma sample was pre-treated with acetonitrile (containing 0.05% formic acid). Chromatographic separation was performed on a kromasil C18 column (250 × 4.6 mm, 5 µm) maintained at 35 °C. The mobile phase was a mixture of methanol and 0.2% formic acid with a step linear gradient. At 1.0 mL min^-1 flow rate, the eluent of other eight flavonoids was detected simultaneously at 360 nm with good separation except genistein (detected at 254 nm). Under optimum conditions, the correlation coefficient between the peak area and the concentrations for each analyte was all above 0.999. The intra-day and inter-day precisions were less than 10% for all analytes. The limit of detection and the limit of quantification for the selected nine flavonoids were 0.006–0.03 and 0.02–0.12 g mL, ^-1 respectively. The extracted recoveries of selected nine flavonoids were 74.02%–99.37%. The assay has been successfully applied to determine concentrations of nine flavonoids in plasma from beagle dog after being intravenously administrated Ginkgo biloba extract.

Słowa kluczowe

EN

high-performance liquid chromatography; beagle dog plasma; flavonoid; Ginkgo biloba extract; pharmacokinetic

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2012
• Tom: Vol. 24, no. 4
• Strony: 627--642
• Opis fizyczny: Bibliogr. 22 poz., rys., tab.

Twórcy

autor

Wu J.

• Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China

autor

Xing H

• Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China

autor

Tang D.

• Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China
• Xuzhou Medical College Department of Pharmaceutical Analysis Xuzhou Jiangsu 221004 China

autor

Gao Y.

• Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China

autor

Yin X

• Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China
• Xuzhou Medical College Department of Pharmaceutical Analysis Xuzhou Jiangsu 221004 China

autor

Du Q.

• Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China

autor

Jiang X.

• Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China
• Xuzhou Medical College Department of Pharmaceutical Analysis Xuzhou Jiangsu 221004 China

autor

Yang D.

• Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China
• Xuzhou Medical College Department of Pharmaceutical Analysis Xuzhou Jiangsu 221004 China

Bibliografia

• [1] M.L. Chavez and P.I. Chavez, Hosp. Pharm. 33, 1076 (1998)
• [2] K. Ndjoko, J.L. Wolfender, and K. Hostettmann, J. Chromatogr. B, 744, 249 (2000)
• [3] R.W. Stackman, F. Eckenstein, B. Frei, D. Kulhanek, J. Nowlin, and J.F. Quinn, Exp. Neurol., 184, 510 (2003)
• [4] M. Ellnain-Wojtaszek, Z. Kruczynski, and J. Krasprzak, Fitoterapia, 74, 1 (2003)
• [5] L.M. Goh, P.J. Barlow, and C.S. Yong, Food Chem., 82, 275 (2003)
• [6] M.L. Chavez and P.I. Chavez, Hosp. Pharm., 33, 658 (1998)
• [7] D.G. Watson and E.J. Oliveira, J. Chromatogr. B, 723, 203 (1999)
• [8] S.M. Boue, C.H. Carter-Weintjies, B.Y. Shih, and T.E. Cleveland, J. Chromatogr. A, 991, 61 (2003)
• [9] O. Sticher, Herbal Medicines for Neuropsychiatric Diseases, Ginkgo Preparations. In: S. Kanba and E. Richelson (Eds) Biochemical, Pharmaceutical, and Medical Perspectives, Seiwa Shoten Publishers, Tokyo, 1999, p. 83
• [10] G.J. Yang, P. Liu, X.L. Qu, M.J. Xu, Q.S. Qu, C.Y. Wang, X.Y. Hu, and Z.Y. Wang, J. Chromatogr. B, 856, 222 (2007)
• [11] F.M. Wang, T.W. Yao, and S. Zeng, J. Pharm. Biomed. Anal., 33, 317 (2003)
• [12] I. Kazuo, F. Takashi, and K. Yasuji, J. Chromatogr. B, 759, 161 (2001)
• [13] Kazuo, F. Takashi, and K. Yasuji, J. Chromatogr. B, 794, 49 (2003)
• [14] Y. Zhao, Y. Sun, and C. Li, J. Am. Soc. Mass Spectrom., 19, 445 (2008)
• [15] F.M. Wang, M.Z. Wu, L. Zhou, T.W. Yao, and Y.H. Yu, China Journal of Chinese Materia Medica, 28, 279 (2003)
• [16] Q.J. Yang, G.Z. Wu, W.J. Xue, Y.N. Tang, and X.Q. Wu, Herald of Medicine, 21, 687 (2002)
• [17] D. Tang, Y. Yin, Z. Zhang, Y. Gao, Y. Wei, Y. Chen, and L. Han, Acta Chromatogr., 21, 483 (2009)
• [18] Food and Drug Administration, Guidance for Industry: Bioanalytical Method Validation, Center for Drug Evaluation and Research, Rockville, MD, 2001, pp. 1–22
• [19] M.J. Dubber and I. Kanfer, J. Pharm. Pharm. Sci., 7, 303 (2004)
• [20] A.G. Jensen, K. Ndjoko, J.L. Wolfender, K. Hostettmann, F. Camponovo, and F. Soldati, Phytochem. Anal., 13, 31 (2002)
• [21] J.X. Chen and Y. Zhang, J. Chromatogr. Sci., 46, 117 (2008)
• [22] D.Q. Tang, Y.G. Chen, D.Z. Yang, Y.Y. Gao, A.B. Tang, X.L. Jiang, and X.X. Yin, Lat. Am. J. Pharm., 29, 853 (2010)