Pharmacokinetics and bioavailability of hapepunine in mice by ultra-performance liquid chromatography–tandem mass spectrometry

• Autorzy: Chen Lianguo , Zhang Qingwei , Lin Yijing , Lu Xiaojie , Zhong Zuoquan , Ma Jianshe , Wen Congcong , Ding Cheng

Identyfikatory

DOI

10.1556/1326.2019.00559

• Języki publikacji: EN

Abstrakty

EN

An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was established to determine the hapepunine in mouse blood, and the pharmacokinetics of hapepunine after intravenous (1.0 mg/kg) and intragastric (2.5, 5, and 10 mg/kg) administrations was studied. Delavinone was used as an internal standard. The UPLC ethylene bridged hybrid (BEH) C18 column was used for chromatographic separation. The mobile phase consisted of acetonitrile and 0.1% formic acid with a gradient elution flow rate of 0.4 mL/min. Multiple reaction monitoring (MRM) mode was used for quantitative analysis of hapepunine in electrospray ionization (ESI) positive interface. Proteins from mouse blood were removed by acetonitrile precipitation. The verification method was established in accordance with the US Food and Drug Administration (FDA) bioanalytical method validation guidelines. In the concentration range of 1–1000 ng/mL, the hapepunine in the mouse blood was linear (r2 > 0.995), and the lower limit of quantification was 1.0 ng/mL. In the mouse blood, the intra-day precision coefficient of variation (CV) was less than 12%, the inter-day precision CV was less than 14%. The accuracy ranged from 91.7% to 109.3%. The average recovery was higher than 76.7%, and the matrix effect was between 86.0% and 106.4%. The UPLC–MS/MS method was sensitive, rapid, and selective and was successfully applied to the pharmacokinetic study of hapepunine in mice. The absolute bioavailability of hapepunine was 22.0%.

Słowa kluczowe

EN

hapepunine; pharmacokinetics; bioavailability; mouse

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2020
• Tom: Vol. 32, no. 1
• Strony: 44--48
• Opis fizyczny: Bibliogr. 22 poz., rys., tab.

Twórcy

autor

Chen Lianguo

• Wenzhou People's Hospital, Wenzhou 325000, China

autor

Zhang Qingwei

• China State Institute of Pharmaceutical Industry, Shanghai 201203, China

autor

Lin Yijing

• China State Institute of Pharmaceutical Industry, Shanghai 201203, China

autor

Lu Xiaojie

• China State Institute of Pharmaceutical Industry, Shanghai 201203, China

autor

Zhong Zuoquan

• China State Institute of Pharmaceutical Industry, Shanghai 201203, China

autor

Ma Jianshe

• Wenzhou Medical University, Wenzhou 325035, China

autor

Wen Congcong

• Wenzhou Medical University, Wenzhou 325035, China

autor

Ding Cheng

• The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China

Bibliografia

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Uwagi

PL

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).