Equalisation of archival microscopic images from immunohistochemically stained tissue sections

• Autorzy: Neumann U. , Korzyńska A. , Lopez C. , Lejeune M. , Roszkowiak Ł. , Bosch R.
• Języki publikacji: EN

Abstrakty

EN

A method of image equalisation that reduces non-uniformity of light distribution caused by optical devices and dust on camera sensors is presented. The method explores non-uniformity which occurs in archival images captured by a typical optical set which consists of a light microscope and a digital camera. A sufficient number of images with low density of foreground objects has been used to extract a global map of non-uniformity of the particular microscope and camera. The proposed method consists of two steps: – (1) extraction of the map of non-uniformity based upon a set of chosen images and – (2) correction of images acquired by the optical set. The global map is created based upon a modified value layer, the third layer of HSV colour space. The proposed method has been tested on images of immunohistochemically (IHC) stained samples of a biopsy tissue, and it has been validated using an image segmentation method developed earlier. The results of the light distribution equalization, as well as the equalized images segmentation turn out to be more similar to the reference method results (namely the manual counting results), than the results of the original images segmentation. The equalization method can be used for other types of images, but all of them should be acquired by the same optical set.

Słowa kluczowe

EN

immunohistochemically stained images; image light distribution; image equalization

PL

obraz mikroskopowy; wyrównanie obrazu

• Wydawca: Nałęcz Institute of Biocybernetics and Biomedical Engineering of the Polish Academy of Sciences ; Elsevier
• Czasopismo: Biocybernetics and Biomedical Engineering
• Rocznik: 2013
• Tom: Vol. 33, no. 1
• Strony: 63--76
• Opis fizyczny: Bibliogr. 54 poz., rys.

Twórcy

autor

Neumann U.

• Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Trojdena 4, 02-109 Warsaw, Poland; University of Nottingham, Jubilee Campus, Nottingham, United Kingdom

autor

Korzyńska A.

• Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Trojdena 4, 02-109 Warsaw, Poland

autor

Lopez C.

• Molecular Biology and Research Section Hospital de Tortosa Verge de la Cinta, Tortosa, Spain

autor

Lejeune M.

• Molecular Biology and Research Section Hospital de Tortosa Verge de la Cinta, Tortosa, Spain

autor

Roszkowiak Ł.

• Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Trojdena 4, 02-109 Warsaw, Poland

autor

Bosch R.

• Molecular Biology and Research Section Hospital de Tortosa Verge de la Cinta, Tortosa, Spain

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