Effect of preservation methods on tensile properties of human femur-ACL-tibial complex (FATC) – a cadaveric study on male subjects

• Autorzy: Marieswaran M. , Mansoori N. , Digge V. K. , Jhahjhria S. K. , Behera C. , Lalwani S. , Kalyanasundaram D.

Identyfikatory

DOI

10.5277/ABB-01134-2018-03

• Języki publikacji: EN

Abstrakty

EN

Deep freezing and storing in formalin are some of the common techniques of human tissue preservation. However, the preservation modes affect the biomechanical properties of the tissues. In this work, the effects of the above-stated preservation techniques are compared with that of fresh cadaveric samples. Methods: FATC samples from male cadavers of age between 60 and 70 years were tested under tensile loading at a strain rate of 0.8 s–1. Fourteen FATC samples from soft embalmed cadavers were preserved for 3 weeks by two methods: (a) 10% formalin and (b) deep freezing at –20 C followed by thawing. Seven FATC samples from fresh cadavers were experimented as control samples. The results were evaluated by a two-stage statistical process of Kruskal–Wallis H test and Mann–Whitney U-test. Results: It was observed that the failure force of fresh cadavers was the highest while that of preserved samples were approximately half the value. Failure elongation of frozen samples exceeded fresh samples while formalin samples failed at lesser elongations. Higher incidence of tibial insertion point or mid-section failures were observed in fresh samples while the higher incidence of ruptures at femoral insertion point was observed in the two preservation methods. Conclusion: Tensile properties of fresh tissues vary significantly from that of formalin preserved or frozen preserved samples.

Słowa kluczowe

EN

tissue preservation; storage in formalin; deep freezing; FATC; anterior cruciate ligament; biomechanical study; tensile testing

PL

przechowywanie w formalinie; mrożenie głębokie; FATC; więzadło krzyżowe przednie; rozciąganie

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2018
• Tom: Vol. 20, no. 3
• Strony: 31--42
• Opis fizyczny: Bibliogr. 20 poz., rys., tab., wykr.

Twórcy

autor

Marieswaran M.

• Centre for Biomedical Engineering, Indian Institute of Technology (IIT) Delhi, New Delhi, India

autor

Mansoori N.

• Cadaver Training and Research Facility (CTRF), Jai Prakash Narayan Apex Trauma Center (JPNTC) – All India Institute of Medical Sciences (AIIMS), New Delhi, India

autor

Digge V. K.

• Department of Orthopaedics, AIIMS, New Delhi, India

autor

Jhahjhria S. K.

• Department of Anatomy, AIIMS, New Delhi, India

autor

Behera C.

• Department of Forensic Medicine, JPNTC, AIIMS, New Delhi, India

autor

Lalwani S.

• Cadaver Training and Research Facility (CTRF), Jai Prakash Narayan Apex Trauma Center (JPNTC)
• Department of Forensic Medicine, JPNTC, AIIMS, New Delhi, India

autor

Kalyanasundaram D.

• Centre for Biomedical Engineering, Indian Institute of Technology (IIT) Delhi, New Delhi, India
• Department of Biomedical Engineering, AIIMS, New Delhi, India

Bibliografia

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).