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Tytuł artykułu

Effect of preservation methods on tensile properties of human femur-ACL-tibial complex (FATC) – a cadaveric study on male subjects

Treść / Zawartość
Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Deep freezing and storing in formalin are some of the common techniques of human tissue preservation. However, the preservation modes affect the biomechanical properties of the tissues. In this work, the effects of the above-stated preservation techniques are compared with that of fresh cadaveric samples. Methods: FATC samples from male cadavers of age between 60 and 70 years were tested under tensile loading at a strain rate of 0.8 s–1. Fourteen FATC samples from soft embalmed cadavers were preserved for 3 weeks by two methods: (a) 10% formalin and (b) deep freezing at –20 C followed by thawing. Seven FATC samples from fresh cadavers were experimented as control samples. The results were evaluated by a two-stage statistical process of Kruskal–Wallis H test and Mann–Whitney U-test. Results: It was observed that the failure force of fresh cadavers was the highest while that of preserved samples were approximately half the value. Failure elongation of frozen samples exceeded fresh samples while formalin samples failed at lesser elongations. Higher incidence of tibial insertion point or mid-section failures were observed in fresh samples while the higher incidence of ruptures at femoral insertion point was observed in the two preservation methods. Conclusion: Tensile properties of fresh tissues vary significantly from that of formalin preserved or frozen preserved samples.
Rocznik
Strony
31--42
Opis fizyczny
Bibliogr. 20 poz., rys., tab., wykr.
Twórcy
  • Centre for Biomedical Engineering, Indian Institute of Technology (IIT) Delhi, New Delhi, India
autor
  • Cadaver Training and Research Facility (CTRF), Jai Prakash Narayan Apex Trauma Center (JPNTC) – All India Institute of Medical Sciences (AIIMS), New Delhi, India
autor
  • Department of Orthopaedics, AIIMS, New Delhi, India
  • Department of Anatomy, AIIMS, New Delhi, India
autor
  • Department of Forensic Medicine, JPNTC, AIIMS, New Delhi, India
autor
  • Cadaver Training and Research Facility (CTRF), Jai Prakash Narayan Apex Trauma Center (JPNTC)
  • Department of Forensic Medicine, JPNTC, AIIMS, New Delhi, India
  • Centre for Biomedical Engineering, Indian Institute of Technology (IIT) Delhi, New Delhi, India
  • Department of Biomedical Engineering, AIIMS, New Delhi, India
Bibliografia
  • [1] AJITA R., SINGH Y.I., Body Donation and Its Relevance in Anatomy Learning – A Review, J. Anat. Soc. India, 2007, 56 (1), 44–47.
  • [2] BEDINO J.H., Embalming Chemistry : Glutaraldehyde versus Formaldehyde, J.H. Bedino, Res. Educ. Dep. Champion Co., 2003, 2614–2632.
  • [3] BRENNER E., Human body preservation – old and new techniques, J. Anat., 2014, 224(3), 316–344.
  • [4] CAWLEY A.T., FLENKER U., The application of carbon isotope ratio mass spectrometry to doping control, J. Mass Spectrom., 2008, 43(7), 854–864.
  • [5] COGLIANO V.J., BAAN R., STRAIF K., GROSSE Y., LAUBY- SECRETAN B. et al., Preventable exposures associated with human cancers, J. Natl. Cancer Inst. 2010, 103(24), 1827–1839.
  • [6] DIMENSTEIN I.B., A Pragmatic Approach to Formalin Safety in Anatomical Pathology, Lab. Med., 2009, 40 (12), 740–746.
  • [7] FALLIS A., Fundamentals of Protein Structure and Function, 2013, Vol. 53, 1689–1699.
  • [8] FESSEL G., FREY K., SCHWEIZER A., CALCAGNI M., ULLRICH O., SNEDEKER J.G., Suitability of Thiel embalmed tendons for biomechanical investigation, Ann. Anat., 2011, 193 (3), 237–241.
  • [9] FRATZL P., WEINKAMER R., Nature’s hierarchical materials, Prog. Mater. Sci., 2007, 52(8), 1263–1334.
  • [10] GUSTAVSON K.H., Aldehyde Tanning in the chemistry of tanning process, Academic Press, New York 1956, 244–282.
  • [11] KAST J., KLOCKENBUSCH C., Optimization of formaldehyde cross-linking for protein interaction analysis of non-tagged integrin, J. Biomed. Biotechnol., 2010.
  • [12] KIERNAN J., Formaldehyde, formalin, paraformaldehyde and glutaraldehyde: what they are and what they do, Micros. Today, 2000, 12 (c), 8–12.
  • [13] MAYER R.G., Embalming History Theory and Practice, 5th ed., The Mc-Graw Hill Companies, 2012.
  • [14] OTTONE N.E., VARGAS C.A., FUENTES R., SOL M. DEL., Walter Thiel’s Embalming Method. Review of Solutions and Applications in Different Fields of Biomedical Research, Int. J. Morphol., 2016, 34(4), 1442–1454.
  • [15] PUCHTLER H., MEIOAN S.N., On the chemistry of formaldehyde fixation and its effects on immunohistochemical reactions, 1985, 201–204.
  • [16] SURI A., ROY T.S., LALWANI S., DEO R.C., TRIPATHI M. et al., Practical guidelines for setting up neurosurgery skills training cadaver laboratory in, 2014, 5–12.
  • [17] THAVARAJAH R., MUDIMBAIMANNAR V., RAO U., RANGANATHAN K., ELIZABETH J., Chemical and physical basics of routine formaldehyde fixation, J. Oral Maxillofac. Pathol., 2012, 16 (3), 400.
  • [18] TORTORA G.J., DERRICKSON B., Principles of Anatomy and Physiology, John Wiley & sons Inc., 12th ed., 2009.
  • [19] V SB. Law in relation to medical men, [in:] Medical Jurisprudence and Toxicology, Rai Bahadur Jaising, P. Modi (Eds.), 22nd ed., Butterworths, New Delhi 1999, 724–727.
  • [20] YONG-HING C.J., OBENAUS A., STRYKER R., TONG K., SARTY G.E., Magnetic resonance imaging and mathematical modeling of progressive formalin fixation of the human brain, Magn. Reson. Med., 2005, 54 (2), 324–332.
Uwagi
Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-9d623fda-c68c-47ed-ae60-0523d47d86f3
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