Determination of polycyclic aromatic hydrocarbons in edible oil by gel permeation chromatography and ultra-high performance liquid chromatography coupled with diode array detector and fluorescence detector

• Autorzy: Wang J. , Jia L. , Wei W. , Lang S. , Shao P. , Fan X.
• Języki publikacji: EN

Abstrakty

EN

This paper develops an instrumental analytical approach for detection of fourteen polycyclic aromatic hydrocarbons (PAHs) in edible oil samples using gel permeation chromatography (GPC) and ultra-high performance liquid chromatography (UHPLC) coupled with diode array detector (DAD), and fluorescence detector (FLD). The GPC was used to remove triglycerides from edible oil samples. The extracted samples were then detected using UHPLC—DAD—FLD. In order to obtain good separation and high reproducibility, the UHPLC—DAD—FLD experimental condition was optimized. The PAHs including three groups of isomeric PAHs can be separated completely in 12 min using BEH Shield RP 18 column with a suitable gradient elution program. The mean recoveries were in the range of 73–110% with an acceptable reproducibility (RSD < 10%, n = 3). During real sample analysis, the method can decrease the chance of false positives with both DAD and FLD being used simultaneously. The results indicate that the approach is simple, easy, and acceptably reproducible, thereby showing great potential as a method for detection of fourteen PAHs contained in edible oil samples.

Słowa kluczowe

EN

diode array detector (DAD); fluorescence detector (FLD); polycyclic aromatic hydrocarbons (PAHs); ultra-high performance liquid chromatography

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2016
• Tom: Vol. 28, no. 3
• Strony: 415--427
• Opis fizyczny: Bibliogr. 19 poz., rys., tab.

Twórcy

autor

Wang J.

• Beijing Centre for Physical and Chemical Analysis, Beijing Engineering Research Center of Food Safety Analysis, Beijing 100089, China

autor

Jia L.

• Beijing Centre for Physical and Chemical Analysis, Beijing Engineering Research Center of Food Safety Analysis, Beijing 100089, China

autor

Wei W.

• Beijing Centre for Physical and Chemical Analysis, Beijing Engineering Research Center of Food Safety Analysis, Beijing 100089, China

autor

Lang S.

• Beijing Centre for Physical and Chemical Analysis, Beijing Engineering Research Center of Food Safety Analysis, Beijing 100089, China

autor

Shao P.

• Beijing Centre for Physical and Chemical Analysis, Beijing Engineering Research Center of Food Safety Analysis, Beijing 100089, China

autor

Fan X.

• Beijing Centre for Physical and Chemical Analysis, Beijing Engineering Research Center of Food Safety Analysis, Beijing 100089, China

Bibliografia

• [1] W. C. Tseng, P. S. Chen, and S. D. Huang, Talanta, 120, 425 (2014)
• [2] H. Alomirah, S. A. Zenki, S. A. Hooti, S. Zaghloul, W. Sawaya, N. Ahmedb, and K. Kannan, Food Control, 22, 2028 (2011)
• [3] Z. H. Xia, X. L. Duan, W. X. Qiu, D. Liu, B. Wang, S. Tao, Q. J. Jiang, B. Lu, Y. X. Song and X. X. Hu, Sci. Total Environ., 408, 5331 (2010)
• [4] S. Wu and W. J. Yu. Food Chem., 134, 597 (2012)
• [5] L. R. Salgueiro, M. S. G. Falcón, E. M. Carballo, and J. S. Gándara, Food Chem., 108, 607 (2008)
• [6] M. K. Pandey, K. K. Mishra, S. K. Khanna, and M. Das, J. Am. Oil Chem. Soc., 81, 1131 (2004)
• [7] S. Y. Chung, R. R. Yettella, J. S. Kim, K. Kwon, M. C. Kim, and D. B. Min, Food Chem., 129, 1420 (2011)
• [8] D. Luo, Q. Yu, H. Yin, and Y. Feng, Anal. Chim. Acta, 588, 261 (2007)
• [9] A. Barranco, R. M. Alonso-Salces, E. Corta, L. A. Berrueta, B. Gallo, F. Vicente, and M. Sarobe, Food Chem., 86, 465 (2004)
• [10] A. Farhadian, S. Jinap, A. Faridah, and I. S. M. Zaidul, Food Control, 28, 420 (2012)
• [11] M. J. Bogusz, S. A. El Hajj, Z. Ehaideb, H. Hassan, and M. Al-Tufail, J. Chromatogr. A, 1026, 1 (2004)
• [12] A. Barranco, R. M. Alonso-Salces, A. Bakkali, L. A. Berrueta, B. Gallo, F. Vicente, and M. Sarobe, J. Chromatogr. A, 998, 33 (2003)
• [13] D. Luo, Q. Yu, H. Yin, and Y. Feng, Anal. Chim. Acta, 588, 261 (2007)
• [14] S. Vichi, L. Pizzale, L. S. Conte, S. Buxaderas, and E. Lopez-Tamames, J. Chromatogr. A, 1090, 146 (2005)
• [15] G. Purcaro, P. Morrison, S. Moret, L. S. Conte, and P. J. Marriott, J. Chromatogr. A, 1161, 284 (2007)
• [16] E. Ballesteros, A. G. Sanchez, and N. R. Martos, J. Chromatogr. A, 1111, 89 (2006)
• [17] Y. Q. Ma, K. Y. Cui, F. Zeng, J. X. Wen, H. Liu, F. Zhu, G. F. Ouyang, T. G. Luan, and Z. X. Zeng. Anal. Chim. Acta, 786, 47 (2013)
• [18] J. Djinovic, A. Popovic, and W. Jira, Eur. Food Res. Technol., 227, 1191 (2008)
• [19] Y. H. Zhu, B. Zhao, R. Q. Xiao, W. Yun, Z. J. Xiao, D. W. Tu, and S. Q. Chen, Food Chem., 15, 956 (2014)

Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.