Fluorescencja multispektralna i metody chemometryczne w zastosowaniach (bio)analitycznych

• Autorzy: Głowacz Klaudia , Skorupska Sandra , Grabowska-Jadach Ilona , Ciosek-Skibińska Patrycja

Identyfikatory

DOI

10.53584/WIADCHEM.2025.11.9

Warianty tytułu

EN

Multispectral fluorescence and chemometric methods in (bio)analytical applications

• Języki publikacji: PL

Abstrakty

EN

The design of methods based on chemical “fingerprints” (a set of analytical signals) and chemometric tools is an important trend of novel (bio)analysis, aimed at reducing multistep sample preparation and the use of complex analytical instrumentation, while increasing the accessibility of (bio)analytical methods. The excitation-emission matrix (EEM) acquired during the multispectral fluorescence measurements is a potentially rich source of chemical information, serving as a unique, fluorescent "fingerprint" of the sample under investigation. This work summarizes the research on the generation and analysis of chemical “fingerprints”, conducted as part of the doctoral thesis of Klaudia Głowacz, PhD, Eng., under the supervision of Prof. Patrycja Ciosek-Skibińska, PhD, DSc, Eng. The research aimed to develop (bio)analytical methods based on fluorescence excitation-emission spectra and chemometric methods for various applications, including monitoring cell viability in adherent cell cultures, detecting neurotransmitters, amino acids, and oligopeptides, as well as amyloid-β derivatives associated with Alzheimer's disease. This article will discuss in detail the results of research on the development of analytical strategies for selected biological samples – cell cultures – to determine cell viability.

Słowa kluczowe

PL

fluorescencja multispektralna; widma wzbudzenia-emisji; metody chemometryczne; hodowle komórkowe; żywotność komórkowa

EN

multispectral fluorescence; excitation-emission matrix; chemometrics; cell culture; cell viability

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Wiadomości Chemiczne
• Rocznik: 2025
• Tom: [Z] 79, 11-12
• Strony: 1079--1096
• Opis fizyczny: Bibliogr. 47 poz., rys., wykr.

Twórcy

autor

Głowacz Klaudia

• Katedra Biotechnologii Medycznej, Wydział Chemiczny, Politechnika Warszawska, Noakowskiego 3, 00-664 Warszawa

• https://orcid.org/0000-0002-7293-2240

autor

Skorupska Sandra

• Katedra Biotechnologii Medycznej, Wydział Chemiczny, Politechnika Warszawska, Noakowskiego 3, 00-664 Warszawa

• https://orcid.org/0000-0002-1576-6485

autor

Grabowska-Jadach Ilona

• Katedra Biotechnologii Medycznej, Wydział Chemiczny, Politechnika Warszawska, Noakowskiego 3, 00-664 Warszawa

• https://orcid.org/0000-0002-3899-267X

autor

Ciosek-Skibińska Patrycja

• Katedra Biotechnologii Medycznej, Wydział Chemiczny, Politechnika Warszawska, Noakowskiego 3, 00-664 Warszawa

• https://orcid.org/0000-0002-8265-1039

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