Emergence of Antibiotic Resistance Genes sul1, tetA, blaGES, and mexF in Sapon Irrigation Canal and Aquaculture Pond in Kulon Progo Regency, Indonesia

• Autorzy: Ikhrami Mohamad Aji , Sari Dini Wahyu Kartika , Putra Masagus Muhammad Prima

Identyfikatory

DOI

10.12911/22998993/176207

• Języki publikacji: EN

Abstrakty

EN

Antibiotic resistance genes (ARGs) have recently become an emerging environmental contaminants. The aquatic environment, such as a river has already become the most polluted environment and can be a driver of ARGs. The water from irrigation canal has the potential to become a hotspot of ARGs through contamination from river pollutants carried along to the irrigation canal. However, the information regarding the cross-contamination of ARGs in fish farming systems integrated with irrigation canal in Indonesia needs further study. This study investigated the occurrence of ARGs sulfonamide (sul1), tetracycline (tetA), beta lactam (bla_GES), and multi drug resistance (mexF) from body water samples along the irrigation canal and aquaculture ponds which utilize irrigation water for cultivation. Sampling sites are located in the Kulon Progo Regency (Indonesia) and samples were taken during the rainy season. Gene amplification was performed using Multiplex PCR. The results showed that sul1, tetA, and bla_GES were detected in 67%, 63%, and 55% of all samples. Meanwhile, mexF was only found upstream and downstream irrigation canals, which accounted for 25% of the total samples. The results of this study indicated that the Sapon Irrigation Canal has the potential to cause the spread of antibiotic resistance genes.

Słowa kluczowe

EN

antibiotic resistance gene; aquaculture; aquatic environment; irrigation canal; multiplex PCR

• Wydawca: Polskie Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology
• Czasopismo: Journal of Ecological Engineering
• Rocznik: 2024
• Tom: Vol. 25, nr 2
• Strony: 85--92
• Opis fizyczny: Bibliogr. 30 poz., rys., tab.

Twórcy

autor

Ikhrami Mohamad Aji

• Master in Biotechnology Study Program, Graduate School, Universitas Gadjah Mada, Jalan Teknika Utara, Pogung, Sinduadi, Mlati, Yogyakarta 55284, Indonesia
• Biotechnology Research Center, Universitas Gadjah Mada, Jalan Teknika Utara, Pogung, Sinduadi, Mlati, Yogyakarta 55284, Indonesia

autor

Sari Dini Wahyu Kartika

• Master in Biotechnology Study Program, Graduate School, Universitas Gadjah Mada, Jalan Teknika Utara, Pogung, Sinduadi, Mlati, Yogyakarta 55284, Indonesia
• Biotechnology Research Center, Universitas Gadjah Mada, Jalan Teknika Utara, Pogung, Sinduadi, Mlati, Yogyakarta 55284, Indonesia
• Aquaculture Laboratory, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jalan Flora A4, Bulaksumur, Yogyakarta 55281, Indonesia

autor

Putra Masagus Muhammad Prima

• Fish Product Technology Study Program, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jalan Flora A4, Bulaksumur, Yogyakarta 55281, Indonesia

• https://orcid.org/0000-0002-1866-4396

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2024).