UHPLC–Q/Orbitrap/MS/MS fingerprinting of Bai-Hu-Jia-Ren-Shen-Tang Decoction and evaluation of its antioxidant activity in streptozotocin-induced diabetic rats

• Autorzy: Wang Libin , Shen Xin , Wang Fang , Xu Xiaohui

Identyfikatory

DOI

10.1556/1326.2022.01068

• Języki publikacji: EN

Abstrakty

EN

Bai-Hu-Jia-Ren-Shen-Tang Decoction (BHJRSTD) is one of the oldest classic Chinese medicine prescriptions which used in the field of treatment of diabetes. However, to the best of our knowledge, the ingredients of this prescription have not been identified, and there are very few studies on the anti-diabetic mechanism of this prescription. Therefore, BHJRSTD was detected and identified by ultra-high-performance liquid chromatography coupled with Quadrupole-Exactive Focus Orbitrap MS (UHPLC–Q/Orbitrap/MS/MS). We identified 74 compounds, including flavonoids, alkaloids, chalcones, xanthones, phenols, phenylpropanoids, terpenes, triterpenes, amino acid derivatives, etc. Then, Sprague Dawley rats were fed with a high-fat and high-sugar diet for two months and injected with streptozotocin (STZ) to induce type 2 diabetes (T2DM). The diabetic rats were randomized to given metformin (200 mg kg⁻¹•d⁻¹, n = 15), BHJRSTD extracts (40 g kg⁻¹•d⁻¹) and BHJRSTD extracts (10 g kg⁻¹•d⁻¹) by gavage for 8 weeks. The results confirmed that BHJRSTD significantly decreased the level of MDA and increased levels of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), it shows that the prescription has significant antioxidant activity in the treatment of T2DM.

Słowa kluczowe

EN

Bai-Hu-Jia-Ren-Shen-Tang Decoction; UHPLC–Q/Orbitrap/MS/MS; Type 2 diabetes; antioxidant activity

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2023
• Tom: Vol. 35, no. 4
• Strony: 293--301
• Opis fizyczny: Bibliogr. 18 poz., rys., tab., wykr.

Twórcy

autor

Wang Libin

• School of Medicine, Shaanxi Energy Institute, Xianyang, Shaanxi Province 712000, China

autor

Shen Xin

• Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, China

autor

Wang Fang

• School of Medicine, Shaanxi Energy Institute, Xianyang, Shaanxi Province 712000, China

• https://orcid.org/0000-0003-3976-3673

autor

Xu Xiaohui

• Department of Orthopaedics, QILU Hospital of Shandong University Dezhou Hospital, DeZhou, Shandong Province 253000, China

• https://orcid.org/0000-0003-2514-4759

Bibliografia

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