Screening of 17α-ethynylestradiol and non-steroidal anti-inflammatory pharmaceuticals accumulation in Mytilus edulis trossulus (Gould, 1890) collected from the Gulf of Gdańsk

• Autorzy: Caban M. , Szaniawska A. , Stepnowski P.

Identyfikatory

DOI

10.1515/ohs-2016-0050

• Języki publikacji: EN

Abstrakty

EN

Pharmaceuticals are commonly found in water collected from wastewater treatment plants, fresh water and saline water. Their presence in water may result in constant accumulation in living organisms, and consequently a negative impact on those organisms. Abiotic samples collected from the Gulf of Gdańsk were already proven to be polluted by several classes of pharmaceuticals, but the bioaccumulation was not investigated as far. This study presents the first results on the accumulation of pharmaceuticals in Mytilus edulis trossulus (Gould 1890) mussels collected from the Gulf of Gdańsk. The content of target 6 non-steroidal anti-inflammatory pharmaceuticals (ibuprofen, flurbiprofen, diclofenac, paracetamol, naproxen, ketoprofen) and 17α-ethynylestradiol in water and tissue samples was determined. The selected pharmaceuticals (paracetamol, flurbiprofen, 17α-ethynylestradiol) were found only in the largest individuals. The in situ BAFs calculated for paracetamol and flurbiprofen were 2850 and 16154 l kg^-1 (dry weight), respectively, suggesting a high potential for the bioaccumulation of these compounds. The synthetic hormone 17α-ethynylestradiol was also found in the collected mussels at the concentration of 310 ng g^-1 of dry weight. Generally, the examined mussels showed poor condition and it is highly likely that the accumulated pharmaceuticals are one of the important factors contributing to this.

Słowa kluczowe

EN

pharmaceutical residues; pharmaceutical bioaccumulation; Mytilus sp.; 17α-ethinylestradiol; EE2; non-steroidal anti-inflammatory drugs; NSAIDs

• Wydawca: Institute of Oceanography University of Gdańsk
• Czasopismo: Oceanological and Hydrobiological Studies
• Rocznik: 2016
• Tom: Vol. 45, No. 4
• Strony: 605--614
• Opis fizyczny: Bibliogr. 64 poz., schem., tab.

Twórcy

autor

Caban M.

• Department of Environmental Analysis, Institute of Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Wita Stwosza 63, 80-308 Gdańsk, Poland

autor

Szaniawska A.

• Department of Experimental Ecology of Marine Organisms, Institute of Oceanography, Faculty of Oceanography and Geography, University of Gdańsk, Al. M. Piłsudskiego 46, 81-378 Gdynia, Poland

autor

Stepnowski P.

• Department of Environmental Analysis, Institute of Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, ul. Wita Stwosza 63, 80-308 Gdańsk, Poland

Bibliografia

• [1]. Älvarez-Munoz, D., Rodriguez-Mozaz, S., Maulvault, A.L., Tediosi, A., Fernandez-Tejedor, M.V. et al. (2015). Occurrence of pharmaceuticals and endocrine disrupting compounds in macroalgaes, bivalves, and fish from coastal areas in Europe. Environ. Res. 143: 56-64. DOI: 10.1016/j. envres.2015.09.018.
• [2]. Arnot, J.A. & Gobas, S. (2006). A review of bioconcentration factor (BCF) and bioaccumulation factor (BAF) assessments for organic chemicals in aquatic organisms. Environ. Reviews. 14: 257-297. DOI: 10.1139/a06-005.
• [3]. Binelli, A., Della Torre, C., Magni, S. & Parolini, M. (2014). Does zebra mussel (Dreissena polymorpha) represent the freshwater counterpart of Mytilus in ecotoxicoloqical studies? A critical review. Environ. Pollut. 196C: 386-403. DOI: 10.1016/j.envpol.2014.10.023.
• [4]. Binelli, A., Magni, S., Soave, C., Marazzi, F., Zuccato, E. et al. (2014). The biofiltration process by the bivalve D. polymorpha for the removal of some pharmaceuticals and drugs of abuse from civil wastewaters. Ecol. Eng. 71: 710-721. DOI: 10.1016/j.ecoleng.2014.08.004.
• [5]. Borecka, M., Siedlewicz, G., Haliński, Ł.P., Sikora, K., Pazdro, K. et al. (2015). Contamination of the southern Baltic Sea waters by the residues of selected pharmaceuticals: Method development and field studies. Mar. Pollut. Bull. 94: 62-71. DOI: 10.1016/j.marpolbul.2015.03.008.
• [6]. Caban, M., Lis, E., Kumirska, J. & Stepnowski, P. (2015). Determination of pharmaceutical residues in drinking water in Poland using a new SPE-GC-MS(SIM) method based on Speedisk extraction disks and DIMETRIS derivatization. Sci. Total Environ. 538: 402-411. DOI: 10.1016/j.scitotenv.2015.08.076.
• [7]. Caban, M., Mioduszewska, K., Łukaszewicz, P., Migowska, N., Stepnowski, P. et al. (2014). A new silylating reagent - dimethyl(3,3,3-trifluoropropyl)silyldiethylamine - for the derivatisation of non-steroidal anti-inflammatory drugs prior to gas chromatography-mass spectrometry analysis. J. Chromatogr. A 1346: 107-116. DOI: 10.1016/j. chroma.2014.04.054.
• [8]. Camacho-Munoz, D., Martin, J., Santos, J.L., Aparicio, I. & Alonso, E. (2010). Occurrence, temporal evolution and risk assessment of pharmaceutically active compounds in Donana Park (Spain). J. Hazard. Mater. 183: 602-608. DOI: 10.1016/j.jhazmat.2010.07.067.
• [9]. Cleuvers, M. (2003). Aquatic ecotoxicity of pharmaceuticals including the assessment of combination effects. Toxicol. Lett. 142: 185-194. DOI: 10.1016/S0378-4274(03)00068-7.
• [10]. Cubero-Leon, E., Ciocan, C.M., Hill, E.M., Osada, M., Kishida et al. (2010). Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis). Aquat. Toxicol. 98: 178-187. DOI: 10.1016/j.aquatox.2010.02.007.
• [11]. Dabrowska, H., Kopko, O., Turja, R., Lehtonen, K.K., Góra, A. et al. (2013). Sediment contaminants and contaminant levels and biomarkers in caged mussels (Mytilus trossulus) in the southern Baltic Sea. Mar. Environ. Res. 84: 1-9. DOI: 10.1016/j.marenvres.2012.11.001.
• [12]. Edebo, L., Haamer, J., Lindahl, O., Loo, L.O. & Piriz, L. (2000). Recycling of macronutrients from sea to land using mussel cultivation. Int. J. Environ. Pollut.13: 190-207. DOI: 10.1504/ IJEP.2000.002315.
• [13]. Ericson, H., Thorson, G. & Kumblad, L. (2010). Physiological effects of diclofenac, ibuprofen and propranolol on Baltic Sea blue mussels. Aquat. Toxicol. 99: 223-231. DOI: 10.1016/j.aquatox.2010.04.017.
• [14]. Gagné, F., André, C., Fortier, M. & Fournier, M. (2012). Immunotoxic potential of aeration lagoon effluents for the treatment of domestic and hospital wastewaters in the freshwater mussel Elliptio complanata. J. Environ. Sci. 24: 781-789. DOI: 10.1016/S1001-0742(11)60862-0.
• [15]. Gagne, F., Berube, E., Fournier, M. & Blaise, C. (2005). Inflammatory properties of municipal effluents to Elliptio complanata mussels - Lack of effects from anti-inflammatory drugs. Comp. Biochem. Physiol. PartC: Toxicol. Pharmacol. 141: 332-337. DOI: 10.1016/j.cca.2005.06.006.
• [16]. Galassi, S., Bettinetti, R., Neri, M.C., Jeannot, R., Dagnac, T. et al. (2008). A multispecies approach for monitoring persistent toxic substances in the Gulf of Gdańsk (Baltic sea). Ecotoxicol. Environ. Saf. 69: 39-48. DOI: 10.1016/j. ecoenv.2006.11.015.
• [17]. Gonzalez-Rey, M. & Bebianno, M.J. (2012). Does non-steroidal anti-inflammatory (NSAID) ibuprofen induce antioxidant stress and endocrine disruption in mussel Mytilus galloprovincialis? Environ. Toxicol. Pharmacol. 33: 361-371.
• [18]. DOI: 10.1016/j.etap.2011.12.017.
• [19]. Gonzalez-Rey, M. & Bebianno, M.J. (2014). Effects of non-steroidal anti-inflammatory drug (NSAID) diclofenac exposure in mussel Mytilus galloprovincialis. Aquat. Toxicol. 148: 818-831. DOI: 10.1016/j.aquatox.2014.01.011.
• [20]. Goverment of Canada. (1999). Canadian Environmental Protection Act, 1999. Canada Gazette Part III.22. Public Works and Goverments Service, Canada, Ottawa, Ont., Canada. pp. 249.
• [21]. Halldórsson, H.P., Svavarsson, J. & Granmo, A. (2005). The effect of pollution on scope for growth of the mussel (Mytilus edulis L.) in Iceland. Mar. Environ. Res. 59: 47-64. DOI: 10.1016/j.marenvres.2004.02.001.
• [22]. Hallmann, A., Smolarz, K., Konieczna, L., Zabrzańska, S., Belka, M. et al. (2016). LC-MS measurment of free steroids in mussels (Mytilus trossulus) from the southern Baltic Sea. J. Pharm. Biomed. Anal. 117: 311-315. DOI:10.1016/j. jpba.2015.09.013.
• [23]. HELCOM Helsinki Commission (2010). Hazardous substances in the Baltic Sea.
• [24]. Kallio, J.M., Lahti, M., Oikari, A. & Kronberg, L. (2010). Metabolites of the aquatic pollutant diclofenac in fish bile. Environ. Sci. Technol. 44: 7213-7219. DOI: 10.1021/es903402c.
• [25]. Kautsky, N. & Andersson, S. (1997). The Baltic Sea environment, and its sensitivity to pollutants with emphasis on organic tin compounds. KemI (Swedish Chemicals Agency) PM 3/97.
• [26]. Kumirska, J., Migowska, N., Caban, M., Łukaszewicz, P. & Stepnowski, P. (2015). Simultaneous determination of non-steroidal anti-inflammatory drugs and oestrogenic hormones in environmental solid samples. Sci. Total Environ. 508: 498-505. DOI: 10.1016/j.scitotenv.2014.12.020.
• [27]. Lachance, A.A., Myrand, B., Tremblay, R., Koutitonsky, V. & Carrington, E. (2008). Biotic and abiotic factors influencing attachment strength of blue mussels Mytilus edulis in suspended culture. Aquat. Biol. 2: 119-129. DOI: 10.3354/ ab00041.
• [28]. Lara-Martin, P.A., Gonzalez-Mazo, E., Petrovic, M., Barcelo, D. & Brownawell, B.J. (2014). Occurrence, distribution and partitioning of nonionic surfactants and pharmaceuticals in the urbanized Long Island Sound Estuary (NY). Mar. Pollut. Bull. 85: 710-719. DOI: 10.1016/j.marpolbul.2014.01.022.
• [29]. Li, H., Helm, P.A. & Metcalfe, C.D. (2010). Sampling in the great lakes for pharmaceuticals, personal care products, and endocrine-disrupting substances using the passive polar organic chemical integrative sampler. Environ. Toxicol. Chem. 29: 751-762. DOI: 10.1002/etc.104.
• [30]. Lindahl, O., Hart, R., Hernroth, B., Kollberg, S., Loo, L.-O. et al. (2005). Improving Marine Water Quality by Mussel Farming: A Profitable Solution for Swedish Society. J. Hum. Environ. 34: 131-138.
• [31]. McEneff, G., Barron, L., Kelleher, B., Paull, B. & Quinn, B. (2013). The determination of pharmaceutical residues in cooked and uncooked marine bivalves using pressurized liquid extraction, solid-phase extraction and liquid chromatography-tandem mass spectrometry. Anal. Bioanal. Chem. 405: 9509-9521. DOI: 10.1007/s00216-013- 7371-6.
• [32]. McEneff, G., Barron, L., Kelleher, B., Paull, B. & Quinn, B. (2014). A year-long study of the spatial occurrence and relative distribution of pharmaceutical residues in sewage effluent, receiving marine waters and marine bivalves. Sci. Total Environ. 476-477: 317-326. DOI: 10.1016/j. scitotenv.2013.12.123.
• [33]. Migowska, N., Caban, M., Stepnowski, P., Kumirska, J. (2012). Simultaneous analysis of non-steroidal anti-inflammatory drugs and estrogenic hormones in water and wastewater samples using gas chromatography-mass spectrometry and gas chromatography with electron capture detection. Sci. Total Environ. 441: 77-88. DOI: 10.1016/j. scitotenv.2012.09.043.
• [34]. Netherton, M. (2011). Uptake and metabolism of pharmaceuticals in aquatic invertebrates. PhD thesis, University of York.
• [35]. Newell, R.I.E. (2004). Ecosystem influences of natural and cultivated populations of suspension-feeding bivalve molluscs: A review. J. Shellfish Res. 23: 51-61.
• [36]. Nikolaou, A., Meric, S. & Fatta, D. (2007). Occurrence patterns of pharmaceuticals in water and wastewater environments. Anal. Bioanal. Chem. 387: 1225-1234. DOI: 10.1007/s00216- 006-1035-8.
• [37]. Nödler, K., Voutsa, D. & Licha, T. (2014). Polar organic micropollutants in the coastal environment of different marine systems. Mar. Pollut. Bull. 85: 50-59. DOI: 10.1016/j. marpolbul.2014.06.024.
• [38]. Norling, P. & Kautsky, N. (2008). Patches of the mussel Mytilus sp. are islands of high biodiversity in subtidal sediment habitats in the Baltic sea. Aquat. Biol. 4: 75-87. DOI: 10.3354/ab00096.
• [39]. Olenycz, M., Sokołowski, A., Niewińska, A., Wołowicz, M., Namieśnik et al. (2015). Comparison of PCBs and PAHs levels in European coastal waters using mussels from the Mytilus edulis complex as biomonitors: Production and mortality rates. Oceanologia 57(2):196-211. DOI: 10.1016/j. oceano.2014.12.001.
• [40]. Oskarsson, H., Wiklund, A.K.E., Thorsén, G., Danielsson, G. & Kumblad, L. (2014). Community interactions modify the effects of pharmaceutical exposure: A microcosm study on responses to propranolol in Baltic Sea coastal organisms. PLoS One 9. DOI: 10.1371/journal.pone.0093774.
• [41]. Parolini, M., Binelli, A., Cogni, D. & Provini, A. (2010). Multi-biomarker approach for the evaluation of the cyto- genotoxicity of paracetamol on the zebra mussel (Dreissena polymorpha). Chemosphere 79: 489-498. DOI: 10.1016/j.chemosphere.2010.02.053.
• [42]. Parolini, M., Binelli, A. & Provini, A. (2011). Chronic effects induced by ibuprofen on the freshwater bivalve Dreissena polymorpha. Ecotoxicol. Environ. Saf. 74: 1586-1594. DOI:10.1016/j.ecoenv.2011.04.025.
• [43]. Pazdro, K., Borecka, M., Siedlewicz, G., Bia, A. & Stepnowski, P. (2016). Analysis of the Residues of Pharmaceuticals in Marine Environment : State-of the Art , Analytical Problems and Challenges. Current Analytical Chemistry 12. DOI: 10.2174/1573411012666151009193536.
• [44]. Pierscieniak, K., Grzymala, J., Wolowicz, M., Pierścieniak, K., Grzymała, J. et al. (2010). Differences in reproduction and condition of Macoma balthica and Mytilus trossulus in the Gulf of Gdansk (Southern Baltic Sea) under anthropogenic influences. Oceanol. Hydrobiol. St. 39: 17-32. DOI: 10.2478/ v10009-010-0054-0.
• [45]. Pimiä, V., Kankaanpää, H. & Kononen, K. (1997). The first observation of okadaic acid in Mytilus edulis from the Gulf of Finland. Boreal Environ. Res. 2: 381-385.
• [46]. Pojana, G., Gomiero, A., Jonkers, N. & Marcomini, A. (2007). Natural and synthetic endocrine disrupting compounds (EDCs) in water, sediment and biota of a coastal lagoon. Environ. Int. 33: 929-936. DOI: 10.1016/j.envint.2007.05.003.
• [47]. Rainbow, P.S., Wolowicz, M., Fialkowski, W., Smith, B.D. & Sokolowski, A. (2000). Biomonitoring of trace metals in the Gulf of Gdansk, using mussels (Mytilus trossulus) and barnacles (Balanus improvisus). Water Res. 34: 1823-1829. DOI: 10.1016/S0043-1354(99)00345-0.
• [48]. Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) is a European Union regulation. (2007).
• [49]. Riisgard, H.U., Egede, P.P. & Barreiro Saavedra, I. (2011). Feeding Behaviour of the Mussel, Mytilus edulis: New Observations, with a Minireview of Current Knowledge. J. Mar. Biol. 2011: 1-13. DOI: 10.1155/2011/312459.
• [50]. Schmidt, W., O'Rourke, K., Hernan, R. & Quinn, B. (2011). Effects of the pharmaceuticals gemfibrozil and diclofenac on the marine mussel (Mytilus spp.) and their comparison with standardized toxicity tests. Mar. Pollut. Bull. 62: 1389-1395. DOI: 10.1016/j.marpolbul.2011.04.043.
• [51]. Smolarz, K., Wołowicz, M. & Stachnik, M. (2006). First record of the occurrence of "gill disease" in Mytilus edulis trossulus from the Gulf of Gdańsk (Baltic Sea, Poland). J. Invertebr. Pathol. 93: 207-209. DOI: 10.1016/j.jip.2006.07.005.
• [52]. Stewart, M., Olsen, G., Hickey, C.W., Ferreira, B., Jelia, A. et al. (2014). A survey of emerging contaminants in the estuarine receiving environment around Auckland, New Zealand. Sci. Total Environ. 468-469: 202-210. DOI: 10.1016/j.scitotenv.2013.08.039.
• [53]. Strogyloudi, E., Giannakourou, A., Legrand, C., Ruehl, A. & Graneli, E. (2006). Estimating the accumulation and transfer of Nodularia spumigena toxins by the blue mussel Mytilus edulis: An appraisal from culture and mesocosm experiments. Toxicon 48: 359-372. DOI: 10.1016/j. toxicon.2006.05.009.
• [54]. Sui, Q., Cao, X., Lu, S., Zhao, W., Qiu, Z. et al. (2015). Occurrence, sources and fate of pharmaceuticals and personal care products in the groundwater: A review. Emerg. Contam. 1: 14-25. DOI: 10.1016/j.emcon.2015.07.001.
• [55]. Svensen, C., Strogyloudi, E., Riser, C.W., Dahlmann, J., Legrand C. et al. (2005). Reduction of cyanobacterial toxins through coprophagy in Mytilus edulis. Harmful Algae 4: 329-336. DOI: 10.1016/j.hal.2004.06.015.
• [56]. UNEP. (2001). Final act of the conference of plenipotentiaries on the Stockholm convention on persistence organic pollutants. In Proceeding of the Conference of Plenipotentiaries on the Stockholm Convention on Persistent Organic Pollutants, Stockholm, Sweden. United Nations Environmental Programme.
• [57]. U.S. EPA. (1996). Ecological Effects Test Guidelines OPPTS 850.1710 - Oyster BCF.
• [58]. U.S. EPA. (2000). Bioaccumulation testing and interpretation for the purpose of sediment quality assessment status and needs.
• [59]. Washington State Department of Ecology. (2004). Final Report: Washington State Polybrominated Diphenyl Ether (PBDE) Chemical Action Plan. Washington, US.
• [60]. Widdows, J., Donkin, P., Staff, F.J., Matthiessen, P., Law, R.J. et al. (2002). Measurement of stress effects (scope for growth) and contaminat levels in mussels (Mytilus edulis) collected from the Irish Sea. Mar. Environ. Res. 53: 327-356. DOI: 10.1016/S0141-1136(01)00120-9.
• [61]. Widdows, J., Nasci, C. & Fossato, V.U. (1997). Effects of pollution on the scope for growth of mussels (Mytilus galloprovincialis) from the Venice Lagoon, Italy. Mar. Environ. Res. 43: 69-79. DOI: 10.1016/0141-1136(96)00003- 7.
• [62]. Willert, P.L. (2007). Assessment of the pharmaceutical market in Poland after accession to the European Union. Eur. J. Heal. Econ. 8: 347-357. DOI: 10.1007/s10198-006-0032-3.
• [63]. Wolowicz, M. & Sokolowski, A. (2006). Effect of eutrophication on the distribution and ecophysiology of the mussel Mytilus trossulus (Bivalvia) in southern Baltic Sea (the Gulf of Gdansk). Limnol. Oceanogr. 51: 580-590.
• [64]. Xie, Z., Lu, G., Liu, J., Yan, Z., Ma, B. et al. (2015). Occurrence, bioaccumulation, and trophic magnification of pharmaceutically active compounds in Taihu Lake, China. Chemosphere 138: 140-147. DOI: 10.1016/j. chemosphere.2015.05.086.

Uwagi

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.