Validation of specificity in quantitative thin layer chromatography—is spectral correlation a reliable measure of spot purity?

• Autorzy: Kobyłka M. , Komsta Ł.
• Języki publikacji: EN

Abstrakty

EN

The current guidelines of quantitative method development establish a requirement to validate a set of method parameters and the selectivity is one of the most important parameters to be proven. Currently, one of the most frequently used approaches is to measure the spectrum of a spot together with some reference spots and to correlate them. Additionally, several places of the spot (start, middle, end) can be measured and also correlated. In the current paper we perform a simulation done on 170 real spectra with different levels of contamination and noise, establishing the distribution of correlation coefficient. It can be concluded that contamination up to 10% almost cannot be detected by simple correlation measurement, regardless of the noise level. Moreover, at a high noise level there is a very low margin between correlation of different compounds and the correlation of the same (but noisy) spectra. These observations suggest that the spectral correlation cannot be a reliable measure of method specificity and some another chemometric methods need to be searched in further research.

Słowa kluczowe

EN

quantitative determination; selectivity; correlation; peak purity; thin layer chromatography

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2012
• Tom: Vol. 24, no. 3
• Strony: 433--444
• Opis fizyczny: Bibliogr. 17 poz., rys., tab.

Twórcy

autor

Kobyłka M.

• Medical University of Lublin Department of Medicinal Chemistry Jaczewskiego 4 20-090 Lublin Poland

autor

Komsta Ł.

• Medical University of Lublin Department of Medicinal Chemistry Jaczewskiego 4 20-090 Lublin Poland

Bibliografia

• [1] K. Ferenczi-Fodor, Z. Végh, and B. Renger, Trends Anal. Chem., 25, 778 (2006)
• [2] J. Sherma, J. AOAC Int., 93, 754 (2010)
• [3] B. Renger, Z. Végh, and K. Ferenczi-Fodor, J. Chromatogr. A, 1218, 2712 (2011)
• [4] K. Ferenczi-Fodor, Z. Végh, and B. Renger, J. Chromatogr. A, 1218, 2722 (2011)
• [5] ICH Guidelines Q2(R1), Validation of Analytical Procedures—Methodology, Geneva, 2005
• [6] K. Ferenczi-Fodor, B. Renger, and Z. Végh, J. Planar Chromatogr., 23, 173 (2010)
• [7] G. Misztal and Ł. Komsta, J. Planar Chromatogr., 18, 188 (2005)
• [8] Ł. Komsta and G. Misztal, J. AOAC Int., 188, 1517 (2005)
• [9] J. Novakovic, K. Nesmerak, H. Nova, and K. Filka, J. Pharm. Biomed. Anal., 25, 957 (2001)
• [10] U. Hubicka, J. Krzek, H. Woltyńska, and B. Stachacz, J. AOAC Int., 92, 1068 (2009)
• [11] J. Krzek, U. Hubicka, J. Szczepańczyk, A. Kwiecień, and W. Rzeszutko, J. Liq. Chrom. Rel. Tech., 29, 2129 (2006)
• [12] P. Jha, R. Parveen, S. Khan, O. Alam, and S. Ahmad, J. AOAC Int., 93, 787 (2010)
• [13] D.B. Patel and N.J. Patel, Int. J. Chemtech Res., 2, 646 (2010)
• [14] A.A. Shirkhedkar and S.J. Surana, J. AOAC Int., 93, 798 (2010)
• [15] P. Srivastava, N. Tivari, A.K. Yadav, V. Kumar, K. Shanker, R.K. Verma, and M.M. Gupta, J. AOAC Int., 91, 1154 (2008)
• [16] M.V. Mahadik, S.R. Dhaneshwar, and M.J. Kulkami, Eurasian J. Anal. Chem., 2, 30 (2007)
• [17] R. Skibiński, Ł. Komsta, M. Grech-Baran, and A. Gumieniczek. Chem. Pap., 64, 273 (2010)