Determination of monocrotaline and usaramine in rat plasma by UPLC-MS/MS and their pharmacokinetics

• Autorzy: Chen Fan , Wang Ziyue , Xia Xiaoyun , Wang Wanhang , Ma Yizhe , Shen Xiuwei , Zhou Xuzhao , Wen Congcong

Identyfikatory

DOI

10.1556/1326.2023.01160

• Języki publikacji: EN

Abstrakty

EN

An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of monocrotaline and usaramine in rat plasma, to study the plasma drug concentration and pharmacokinetics, and to calculate the absolute bioavailability. The plasma was treated with acetonitrile and methanol (9:1, v/v) protein precipitation method. The chromatographic column was UPLC HSS T3 (50 mm 3 2.1 mm, 1.7 μm), the mobile phase was methanol-water (containing 0.1% formic acid with 10 mM ammonium acetate in water), and the elution time was 4 min at a flow rate of 0.4 mL min1 . Electrospray ionization (ESI) positive ion mode was used for detection and multiple reaction monitoring (MRM) mode was used for quantitative analysis. Monocrotaline and usaramine were administered sublingual intravenously (iv) 1 mg kg1 and orally (po) 5 mg kg1 , respectively, with 6 rats in each group, for a total of 24 rats. Then the pharmacokinetic differences in rats were evaluated. For the UPLC-MS/MS method, the calibration curve showed good linearity in the range of 2–2,000 ng mL1 , where r was greater than 0.99. The precision, accuracy, recovery, matrix effect and stability results were all consistent with the requirements of biological sample detection methods. to provide scientific experimental basis for the basic research The bioavailability of monocrotaline and usaramine in rat plasma was calculated, which was 43.5 and 19.5%, respectively.

Słowa kluczowe

EN

monocrotaline; usaramine; UPLC-MS/MS; pharmacokinetics

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2024
• Tom: Vol. 36, no. 3
• Strony: 256–--262
• Opis fizyczny: Bibliogr. 22 poz. rys., wykr.

Twórcy

autor

Chen Fan

• Ruian People’s Hospital, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, China

autor

Wang Ziyue

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China

autor

Xia Xiaoyun

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China

autor

Wang Wanhang

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China

autor

Ma Yizhe

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China

autor

Shen Xiuwei

• Ruian People’s Hospital, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, China

autor

Zhou Xuzhao

• The Molecular Neuropharmacology Laboratory and the Eye-Brain Research Center, The State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, China

• https://orcid.org/0000-0002-4398-3495

autor

Wen Congcong

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China

• https://orcid.org/0000-0001-8413-1697

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