Phytochemical screening and in vitro antimicrobial properties of Annona muricata extracts against certain human pathogens

• Autorzy: Oluyege J.O. , Orjiakor Paul Ikechukwu , Badejo O.E.

Identyfikatory

DOI

10.17402/390

• Języki publikacji: EN

Abstrakty

EN

All over the world, the use of medicinal plants is gaining more acceptability due to the possibility of discovering novel drugs from them and solving the problem of antimicrobial resistance associated with conventional antibiotics. The phytochemical composition and antimicrobial properties of crude extracts of the leaves, stems, and bark of Annona muricata were evaluated on Escherichia coli, Staphylococcus aureus, and Salmonella typimurium, while the antifungal properties were evaluated against Candida albicans and Candida tropicalis. The Agar well method was used for the study. At concentrations of 150 mg/ml and 300 mg/ml, inhibitory effects were observed on E. coli and S. aureus, with a visible zone of inhibition ranging from 15 mm to 21 mm respectively, and with respect to N- hexane, an antimicrobial activity range of 5 mm to 20 mm, for the leaf extract, which shows effective antimicrobial action against E. coli and S. aureus. Hot water extracts were observed to possess more bioactive compounds compared to organic solvent extracts, and exhibit higher ranges of activity against the tested bacterial species. All extracts exhibited low anti-fungal activity in the range of 8 mm to 15 mm. The phytochemical screening of the extracts of different parts of A. muricata revealed the presence of secondary metabolites such as tannins, alkaloids, saponins, flavonoids, steroids, and cardiac glycosides. The antimicrobial activity of the extracts was compared with a standard antibiotic, ketoconazole, and with ampicillin, which served as the controls). The results showed that A. muricata can be used as an anti-bacterial substance, since it shows broad spectrum activity against a range of bacteria responsible for the most common bacterial illnesses. Further research will be necessary to ascertain its full spectrum of efficacy.

Słowa kluczowe

EN

Annona muricata; pathogens; bacteria; fungi; antibiotics; medicinal plants

• Wydawca: Wydawnictwo Naukowe Akademii Morskiej w Szczecinie
• Czasopismo: Zeszyty Naukowe Akademii Morskiej w Szczecinie
• Rocznik: 2019
• Tom: nr 60 (132)
• Strony: 203--209
• Opis fizyczny: Bibliogr. 30 poz., rys. tab.

Twórcy

autor

Oluyege J.O.

• Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, P.M.B. 5363, Ekiti State, Nigeria

autor

Orjiakor Paul Ikechukwu

• Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, P.M.B. 5363, Ekiti State, Nigeria

autor

Badejo O.E.

• Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, P.M.B. 5363, Ekiti State, Nigeria

Bibliografia

• 1. Abdul Wahab, S.M., Jantan, I., Haque, M.A. & Arshad, L. (2018) Exploring the Leaves of Annona muricata L. as a Source of Potential Anti-inflammatory and Anticancer Agents. Frontiers in Pharmacology 9, 661, pp. 1–20.
• 2. Adeshina, G.O., Onujagbe, O.M. & Onaolapo, J.A. (2009) Comparative Antibacterial Studies on the Root, Stem Bark and Leaf Extracts of Parkia clappertoniana. The Internet Journal of Alternative Medicine 8, 2.
• 3. De S. Luna, J., Dos Santos, A.F., De Lima, M.R., De Omena, M.C., De Mendonça, F.A., Bieber, L.W. & Sant’Ana, A.E. (2005) A study of the larvicidal and molluscicidal activities of some medicinal plants from northeast Brazil. Journal of Ethnopharmacology 97, 2, pp. 199–206.
• 4. Edeoga, H.O., Okwu, D.E. & Mbaebie, B.O. (2005) Phytochemical Constituents of some Nigerian Medicinal Plants. African Journal of Biotechnology 4, pp. 685–688.
• 5. Foong, C.P. & Hamid, R.A. (2012) Evaluation of anti-inflammatory activities of Ethanolic extracts of Annona muricata leaves. Brazilian Journal Pharmacology 22, 6, pp. 1301–1307.
• 6. Gajalakshmi, S., Vijayalakshmi, S. & Rajeswari, D. (2012) Phytochemical and pharmacological Properties of Annona muricata: A review. International Journal of Pharmacy and Pharmaceutical Sciences 4, 2, pp. 3–6.
• 7. Ginda, H., Niky, P.U, & Erly, S. (2014) Study of the antibacterial activities of soursop (Annona muricata) Leaves. International Journal Pharmtechnology Research 6, 2, pp. 575–581.
• 8. Harbone, J.B. (1973) Phytochemical Methods. London: Chapman and Hall Ltd. pp. 49–188.
• 9. Lans, C.A. (2006) Ethno-medicinal used in Trinidad and Tobago for urinary Problems and diabetes mellintus. Journal of Ethnobiology 2, pp. 45–55.
• 10. Le Ven, J., Schmitz-Afonsbo, I., Toubol, D. & Champy, P. (2011) Annonaceace fruits and parkinsonism risk. Metabolisation study of annonacin, a model neurotoxin; evaluation of human exposure. Toxicology letters 205, pp. 50–60.
• 11. Lenk, S.E., Bhat D., Blankency, W. & Andduun, A. (1992) Effects of Streptozotosin Induced diabetes on Rough endoplasmic reticulum and Lysosomes of the rat Liver. American Physiology 263, pp. 856–862.
• 12. Matsushige, A., Kotake, Y. & Takeda, Y. (2012) Annonamine, a new Aporphine alkaloid from leaves of Annona muricata. Chemical and Pharmacuetical Bulletin 60, 2, pp. 257–259.
• 13. Moghadamtousi, S.Z., Fadaeinasab, M., Nikzad, S., Mohan, G., Ali, H.M. & Kadir, H.A. (2015) Annona muricata (Annonaceae): A Review of Its Traditional Uses, Isolated Acetogenins and Biological Activities. International Journal of Molecular Sciences 16, 7, pp. 15625– 15658.
• 14. Ogu, G.I. & Amiebenomo, R. (2012) Phytochemical analysis and in vivo anti-diarrhoeal potentials of Dialium guineense Willd stem bark extract. Journal Intercultural Ethnopharmacology 1, 2, pp. 105–110.
• 15. Ogu, G.I., Ezeadila, J. & Ehiobu, J.M. (2013) Antioxidant and antimicrobial activities of Dialium guineense (Willd) leaf extract. Pharmacy and Pharmacology Research 1, 1, pp. 1–7.
• 16. Ogu, G.I., Tanimowo, W.O., Nwachukwu, P.U. & Igere, B.E. (2012) Antimicrobial and phytochemical evaluation of the leaf, stem bark and root extracts of Cyathula prostrata (L) Blume against some human pathogens. Journal of Intercultural Ethnopharmacology 1, 1, pp. 35–43.
• 17. Padma, P., Chansauria, J.P.N., Khosa, R.L. & Ray, A.K. (2001) Effect of Annona muricata and Pollyalthia cerasoides on brain neurotransmitters and enzyme monoamine oxidase following cold immobilization stress. Journal of Natural Remedies 1, 2, pp. 144–46.
• 18. Padma, P., Chansouria, J. & Khosa, R. (2009) Wound healing Activity of Annona muricata extract. Journal of Pharmacy Research 2, 3, pp. 404–406.
• 19. Parekh, J. & Chanda, S. (2007) In vitro antibacterial activity of the crude Methanol extract of Woodfordia fruiticosa Kurz. Flower (lythraceae). Brazilian Journal of Microbiology 38, pp. 204–207.
• 20. Pathak, P., Saraswathy, D.R., Vora, A. & Savai, J. (2010) In vitro antimicrobial Activity and phytochemical analysis of the leaves of Annona muricata. International Journal of Pharmacology Research and Development 2, 5, pp. 23–45.
• 21. Rarassari, M.A & Maftuch, H.N. (2016) Phytochemicals and Antibacterial Activities of Soursop Leaf (Annona muricata) against Edwardsiella tarda (In Vitro). Journal of Life Science and Biomedicine 6, 1, pp. 06–09.
• 22. Roslida, A.H., Tay, C.E., Zuraini, A. & Chan, P.F. (2010) Anti-inflammatory and anti-nociceptive activities of the ethanolic extract of Annona muricata leaf. Journal of National Remedies 10, 2, pp. 97–104.
• 23. Sarah, I.A. Mustafa, T.M. & Rehab, A.A. (2015) Study on the trace element and some properties of the fruit juice of soursop and their effect on liver enzymes. Journal of Pharmaceutical and Chemical Science 3, 1, pp. 40–45.
• 24. Sasidharan, S., Chen, Y., Saravanana, D., Sundram K.M. & Yoga Latha, L. (2011) Extraction and Characterization of bioactive compound from plants extracts. African Journal Traditional Complementary Alternative Medical 8 (1), pp. 1–10.
• 25. Sukhdev, S., Suman, P., Gennaro, L. & Dev, D. (2008) Extraction technologies for medicinal nd aromatic plants. International Centre for Science and High Technology 23, 6, pp. 34–56.
• 26. Tattersfield, F. (1940) The Insecidal Properties of certain species of Annona and an India strain of mundulea Sericea. Annals Applied Biology 27, pp. 262–73.
• 27. Usonobun, U. & Paulinus, O.N. (2015) Phytochemical Analysis and Mineral composition of Annona muricata leaves. International Journal of Research and Current Development 1, 1, pp. 38–42.
• 28. Vashist, H. & Jindal, A. (2012) Antimicrobial Activities of Medicinal Plants –Review. International Journal of Research in Pharmaceutical Sciences 3, pp. 222–230.
• 29. Vijayameena, C., Subhashini, G., Loganayagi, M. & Ramesh, B. (2013) Phytochemical screening and assessment of antibacterial activity for the bioactive compounds in Annona muricata. Internal Journal Current Microbiology Applied Science 2, 1, pp. 1–8.
• 30. Wicaksono, A., Kalahkan, K., Dengan, S. & Media, M. (2011) Pharmacological Properties of Annona muricata. Journal of Pharmaceutical 19, 23, pp. 102–233.