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The Morphological and Physiological Appearance of Two Vegetable Plants Due to Lead Exposure

Treść / Zawartość
Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
This research aimed to determine the effect of different concentrations of Lead (Pb) on the morphology of kale and spinach plants. The process involved planting kale and spinach seeds in tubs and transferring them to polybags with planting media in the form of soil and sand at a ratio of 4:1 after strong roots were developed. It is important to note that the media were analyzed to ensure the Pb content in the soil was below the threshold before planting. Pb was later provided 1 week before planting in the form of PbNO3 in the media at a dose of 1 and 2 g/polybag and mixed effectively to ensure even distribution, while the sample used as the control was not given any Pb. The transplanting process was conducted after the plants were 18 days old in the nursery and the initial observations at 9 DAT showed that the kale leaves were darker with a score of 3 than spinach with a score of 1, but the spinach leaves became darker in color with score 3 as the age of the plants increased. Moreover, the kale changed to a lighter color with a score of 2 from the 9th day of observation after transplanting, while spinach requires 15 DAT to become score 2 until the end of the observation. It should be pointed out that both plants showed morphological changes due to the existence of the Pb but their base leaves did not reflect any effect. The kale leaf tip became blunt, while the spinach leaf tip was not affected and both plants were discovered to have longer roots and more root hairs in the control compared to the treatments. Furthermore, the total chlorophyll of spinach in the control was higher than kale but observed to reduce as the concentration of Pb increased in the treatments. The morphology and physiology of spinach and kale plants changed due to the Pb exposure with the spinach was discovered to be more sensitive as indicated by more visible morphological damage to its leaves at the end of the observation. It is possible to use the morphology of spinach and kale to detect Pb-contaminated land.
Słowa kluczowe
EN
Rocznik
Strony
213--222
Opis fizyczny
Bibliogr. 30 poz., rys., tab.
Twórcy
  • Faculty of Agriculture, University of Islam Malang, MT. Haryono Street No. 193, Malang, East Java, Indonesia
  • Faculty of Agriculture, University of Islam Malang, MT. Haryono Street No. 193, Malang, East Java, Indonesia
Bibliografia
  • 1. Alexander P.D., Alloway B.J., Dourado A.M. 2006. Genotypic variations in the accumulation of Cd, Cu, Pb and Zn exhibited by six commonly grown vegetables. Environmental Pollution, 144(3), 736–745.
  • 2. Balakhnina T.I., Kosobryukhov A.A., Ivanov A.A., Kreslavskii V.D. 2005. The effect of cadmium on CO2 exchange, variable fluorescence of chlorophyll, and the level of antioxidant enzymes in pea leaves. Russian Journal of Plant Physiology, 52(1), 15–20.
  • 3. Gao Y., Miao C., Mao L., Zhou P., Jin Z., Shi W. 2010. Improvement of phytoextraction and antioxidative defense in Solanum nigrum L. under cadmium stress by application of cadmium-resistant strain and citric acid. Journal of Hazardous Materials, 181(1–3), 771–777.
  • 4. García G., Faz Á., Conesa H.M. 2003. Selection of autochthonous plant species from SE Spain for soil lead phytoremediation purposes. Water, Air and Soil Pollution: Focus, 3(3), 243–250.
  • 5. Hegedüs A., Erdei S., Horváth G. 2001. Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedlings under cadmium stress. Plant science, 160(6), 1085–1093.
  • 6. Huang Y., Ying H., Yunxia L. 2009. Combined toxicity of copper and cadmium to six rice genotypes (Oryza sativa L.). Journal of Environmental Sciences, 21(5), 647–653.
  • 7. Inoue H., Fukuoka D., Tatai Y., Kamachi H., Hayatsu M., Ono M., Suzuki S. 2013. Properties of lead deposits in cell walls of radish (Raphanus sativus) roots. Journal of Plant Research, 126(1), 51–61.
  • 8. Islam E., Yang X., Li T., Liu D., Jin X., Meng F. 2007. Effect of Pb toxicity on root morphology, physiology and ultrastructure in the two ecotypes of Elsholtzia argyi. Journal of Hazardous Materials, 147(3), 806–816.
  • 9. Israr M., Sahi S.V. 2008. Promising role of plant hormones in translocation of lead in Sesbania drummondii shoots. Environmental Pollution, 153(1), 29–36.
  • 10. Jiang W., Liu D. 2010. Pb-induced cellular defense system in the root meristematic cells of Allium sativum L. BMC Plant Biology, 10(1), 1–8.
  • 11. Kumar M., Jayaraman P. 2014. Toxic effect of lead nitrate Pb (NO3)2 on the black gram seedlings (Vigna mungo L. Hepper). International Journal of Advanced Research in Biological Sciences, 1(9), 209–213.
  • 12. Lamhamdi M., El Galiou O., Bakrim A., NóvoaMuñoz J.C., Arias-Estévez M., Aarab A., Lafont R. 2013. Effect of lead stress on mineral content and growth of wheat (Triticum aestivum) and spinach (Spinacia oleracea) seedlings. Saudi Journal of Biological Sciences, 20(1), 29–36.
  • 13. Lelifajri L. 2010. Cu(II) metal ion adsorption using lignin from sawdust waste. Journal of Chemical Engineering & Environmental, 7(3), 126–129.
  • 14. Malar S., Vikram S.S., Favas P.J., Perumal V. 2016. Lead heavy metal toxicity induced changes on growth and antioxidative enzymes level in water hyacinths Eichhornia crassipes Mart.). Botanical Studies, 55(1), 1–11.
  • 15. Malecka A., Piechalak A., Tomaszewska B. 2009. Reactive oxygen species production and antioxidative defense system in pea root tissues treated with lead ions: the whole roots level. Acta Physiologiae Plantarum, 31(5), 1053–1063.
  • 16. Myśliwa-Kurdziel B., Strzałka K. 2002. Influence of metals on biosynthesis of photosynthetic pigments. In Physiology and biochemistry of metal toxicity and tolerance in plants. Springer, Dordrecht, 201–227.
  • 17. Nareshkumar A., Krishnappa B.V., Kirankumar T.V., Kiranmai K., Lokesh U., Sudhakarbabu O., Sudhakar C. 2014. Effect of Pb-stress on growth and mineral status of two groundnut (Arachis hypogaea L.) cultivars. Journal of Plant Sciences, 2(6), 304–310.
  • 18. Naz A., Khan S., Qasim M., Khalid S., Muhammad S., Tariq M. 2013. Metals toxicity and its bioaccumulation in purslane seedlings grown in controlled environment. Natural Science, 5(5), 573–557.
  • 19. Newman E.I. 1966. A method of estimating the total length of root in a sample. Journal of Applied Ecology, 139–145.
  • 20. Piechalak A., Tomaszewska B., Baralkiewicz D., Malecka A. 2002. Accumulation and detoxification of lead ions in legumes. Phytochemistry, 60(2), 153–162.
  • 21. Podlipná R. 2002. Wise, DL, Trantolo, DJ, Cichon, EJ, Inyang, HI, Stottmeister, U.(ed.): Bioremediation of Contaminated Soils. Biologia Plantarum, 45(1), 64–64.
  • 22. Schwarz D., Grosch R. 2003. Influence of nutrient solution concentration and a root pathogen (Pythium aphanidermatum) on tomato root growth and morphology. Scientia Horticulturae, 97(2), 109–120.
  • 23. Sędzik M., Smolik B., Krupa-Małkiewicz M. 2015. Effect of lead on germination and some morphological and physiological parameters of 10-day-old seedlings of various plant species. Environmental Protection and Natural Resources, 26(3), 22–27.
  • 24. Shafiq M., Iqbal M.Z., Mohammad A. 2008. Effect of lead and cadmium on germination and seedling growth of Leucaena leucocephala. Journal of Applied Sciences and Environmental Management, 12(3), 61–66.
  • 25. Sharma P., Dubey R.S. 2005. Lead toxicity in plants. Brazilian journal of plant physiology, 17(1), 35–52.
  • 26. Tennant D. 1975. A test of a modified line intersect method of estimating root length. The Journal of Ecology, 995–1001.
  • 27. Tian T., Ali B., Qin Y., Malik Z., Gill R.A., Ali S., Zhou W. 2014. Alleviation of lead toxicity by 5-aminolevulinic acid is related to elevated growth, photosynthesis, and suppressed ultrastructural damages in oilseed rape. BioMed Research International, 2014, 1–11.
  • 28. Tordoff G.M., Baker A.J.M., Willis A.J. 2000. Current approaches to the revegetation and reclamation of metalliferous mine wastes. Chemosphere, 41(1–2), 219–228.
  • 29. Verma A., Singh S.N. 2006. Biochemical and ultrastructural changes in plant foliage exposed to auto-pollution. Environmental Monitoring and Assessment, 120(1), 585–602.
  • 30. Zhang G.Q., Zhou W.J., Gu H.H., Song W.J., Momoh E.J.J. 2003. Plant regeneration from the hybridization of Brassica juncea and B. napus through embryo culture. Journal of Agronomy and Crop Science, 189(5), 347–350.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-9acd04f0-377a-4330-af0e-144814514d3c
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