A simple HPLC method for determining 2-(3-chlorophenyloamino)-5-(2,4-dihydroxyphenyl)-1,3,4-thiadiazole in brain and plasma of animals: Application to a pharmacokinetic study

• Autorzy: Raszewski G. , Juszczak M. , Lemieszek M. K. , Matysiak J. , Niewiadomy A. , Rzeski W.
• Języki publikacji: EN

Abstrakty

EN

The development, optimization, and validation of a new high-performance liquid chromatography ultraviolet (HPLC-UV) method is presented for determining 2-(3-chlorophenyloamino)-5-(2,4-dihydroxy-phenyl)-1,3,4-thiadiazole (ClABT) in biological samples of rat plasma and brain tissue. ClABT was extracted directly from a plasma supernatant fraction following protein precipitation with acetonitrile and high speed centrifugation. Reverse phase HPLC separation was performed using an ODS-2 Hypersil column with the mobile phase consisting of 0.05 M triethylammonium phosphate buffer solution in acetonitrile and methanol (120:280:600, v/v/v), at room temperature, 1.2 mL min^-1 flow rate, and UV-diode-array detection (DAD), at 335 nm. A linear response was obtained between 12.5 and 2000 ng mL^-1 at analytically acceptable levels of precision (intra/inter-day) and accuracy. Mean recoveries ranged from 92.7% to 107.9%. It was concluded that the method was specific and precise and thus suitable for quantitative analysis in clinical pharmacokinetic studies of ClABT.

Słowa kluczowe

EN

2-(3-chlorophenyloamino)-5-(2 4-dihydroxy-phenyl)-1,3, 4-thiadiazole (ClABT); HPLC-UV; pharmacokinetic study

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 2
• Strony: 255--266
• Opis fizyczny: Bibliogr. 20 poz., rys., tab.

Twórcy

autor

Raszewski G.

• Institute of Agricultural Medicine Department of Pathophysiology Lublin Poland

autor

Juszczak M.

• Institute of Agricultural Medicine Department of Medical Biology Lublin Poland

autor

Lemieszek M. K.

• Institute of Agricultural Medicine Department of Medical Biology Lublin Poland

autor

Matysiak J.

• University of Life Sciences Department of Chemistry Lublin Poland

autor

Niewiadomy A.

• University of Life Sciences Department of Chemistry Lublin Poland

autor

Rzeski W.

• Maria Curie-Sklodowska University Department of Virology and Immunology, Institute of Microbiology and Biotechnology Lublin Poland
• Institute of Agricultural Medicine Department of Medical Biology Lublin Poland

Bibliografia

• [1] N. Siddiqui, P. Ahujaa, W. Ahsana, S.N. Pandeyab, and M. Shamsher, J. Chem. Pharm. Res., 1, 19 (2009)
• [2] F. Vergne, P. Bernardelli, E. Lorthiois, N. Pham, E Proust, Ch. Oliveira, A. Mafroud, F. Roye, R. Wrigglesworth, J.K. Schellhaas, M.R. Barvian, F. Moreau, M. Idrissi, A. Tertre, B. Bertin, M. Coupe, P. Berna, and P. Soulard, Bioorg. Med. Chem. Lett., 14, 4607 (2004)
• [3] K.Y. Jung, S.K. Kim, Z.G. Gao, A.S. Gross, N. Melman, K.A. Jacobson, and Y. Ch. Kim, Bioorg. Med. Chem., 12, 613 (2004)
• [4] P. Bhattacharya, J. Leonard, and K. Roy, Bioorg. Med. Chem., 13, 1159 (2005)
• [5] J. Matysiak and A. Niewiadomy, Synth. Commun. Chem., 36, 1621 (2006)
• [6] W. Rzeski, J. Matysiak, and M. Kandefer-Szerszeń, Bioorg. Med. Chem., 15, 3201 (2007)
• [7] M. Juszczak, J. Matysiak, W. Brzana, A. Niewiadomy, and W. Rzeski, Arzneim. Forsch. Drug Res., 58, 353 (2008)
• [8] M.S. Chernov’yants, I.V. Burykin, and N.V. Aleshina, J. Anal. Chem., 66, 280 (2011)
• [9] J.A. Stewart, C.C. Ackerly, R.A. Newman, and I.H. Krakoff, Cancer Chemother. Pharmacol., 16, 287 (1986)
• [10] E. Tomori, E. Tormasi, M. Varga, and J. Borlak, J. Chromatogr. B Biomed. Sci. Appl., 705, 105 (1998)
• [11] J.F. Borzelleca, J.L. Egle Jr., G.R. Hennigar, H.H. Klein, E.J. Kuchar, R.W. Lane, and P.S. Larson, Toxicol. Appl. Pharmacol., 56, 164 (1980)
• [12] H. Wong, J. Lee, J.B. Camden, and S.D. Weitman, J. Chromatogr. B., 765, 55 (2001)
• [13] Guidance for Industry, Bioanalytical Method Validation, U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (2001)
• [14] Y. Jiang, Y. Zhang, and Z.R. Zhang, J. Chromatogr. B., 791, 285 (2003)
• [15] J.I.D. Wibawa, D. Fowkes, P.N. Shaw, and D.A. Barrett, J. Chromatogr. B., 774, 141 (2002)
• [16] C.L. Cheng, C.H. Chou, and O.Y. Hu, J. Chromatogr. B., 769, 297 (2002)
• [17] M. Zaffaroni, C. Mucignat, T. Pecere, G. Zagotto, R. Frapolli, M D’Incalai, and M. Zucchetti, J. Chromatogr. B., 796, 113 (2003)
• [18] F. Wu, Z. Zhang, and H. Ding, J. Chromatogr. Sci., 44, 12 (2006)
• [19] H. Wong, L. Jia, J.B. Camden, and S.D. Weitman, J. Chromatogr. B., 765, 55 (2001)
• [20] B. Narola, A.S. Singh, P.R. Santhakumar, and T.G. Chandrashekhar, Anal. Chem. Insights., 5, 37 (2010)