Integrin αv signaling influences phenotype and maturation of primary human osteoblasts on alumina surface

• Autorzy: Wróbel E. , Witkowska-Zimny M. , Mrówka P. , Głodkowska-Mrówka E.
• Języki publikacji: EN

Abstrakty

EN

Due to the growing interest in stem cells application in tissue engineering the better understanding of primary human osteoblasts behavior in vitro, on biomaterial surface, is required. Among other molecules integrins may be taken into account as being involved in these phenomena. Integrins are a family of cell adhesion receptors, which may regulate many cellular functions e.g., adhesion, motility, phenotype and cell maturation. The aim of this study was to determine the effect of the biomaterial surfaces and αv integrin signaling pathway on the behavior, phenotype and maturation of human osteoblasts in vitro. Human bone derived cells (HBDCs) obtained from adult femoral bone fragments were cultured on both alumina disks and tissue culture polystyrene (TCPS) dishes. After 7, 14, and 21 days of culture, localization and mRNA expression level of αv integrin subunits and BGLAP (osteocalcin) on polystyrene were analyzed in addition, we treated the cell cultures with monoclonal antibodies against human αv integrin to block its ligand-binding activity, on both alumina and TCPS substrates. We found that the αv integrin was present in focal contacts and cell cytoplasm at subsequent stages of cell maturation and the level of αv integrin mRNA was the highest in mature osteoblasts. Blocking αv integrin transduction pathway caused changes in cell activity and morphology, decreased cells proliferation on TCPS and reduced expression of alkaline phosphatase (ALP) on both materials. The results suggest that αv integrin is involved as an important receptor facilitating osteogenic differentiation.

Słowa kluczowe

EN

integrin; human bone-derived cells; osteogenic differentiation; cell adhesion; biomaterial

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2014
• Tom: Vol. 17, no. 127
• Strony: 33--39
• Opis fizyczny: Bibliogr. 38 poz., tab., wykr., zdj.

Twórcy

autor

Wróbel E.

• Department of Biophysics and Human Physiology, Medical University of Warsaw, 5 Chałubińskiego St., 02-004 Warsaw, Poland

autor

Witkowska-Zimny M.

• Department of Biophysics and Human Physiology, Medical University of Warsaw, 5 Chałubińskiego St., 02-004 Warsaw, Poland

autor

Mrówka P.

• Department of Biophysics and Human Physiology, Medical University of Warsaw, 5 Chałubińskiego St., 02-004 Warsaw, Poland

autor

Głodkowska-Mrówka E.

• Department of Immunology, Center of Biostructure Research, Medical University of Warsaw, 1A Banacha St., 02-097 Warsaw, Poland

Bibliografia

• [1] Athanassiou G., Deligianni D.: Adhesion strength of individual human bone marrow cells to fibronectin. Integrin beta1-mediated adhesion. J Mater Sci Mater Med 12 (2001) 965-970.
• [2] Geiger B., Bershadsky A., Pankov R., Yamada K.M.: Transmembrane crosstalk between the extracellular matrix-cytoskeleton crosstalk. Nat Rev Mol Cell Biol 2 (2001) 793-805.
• [3] Clover J., Dodds R.A., Gowen M.: Integrin subunit expression by human osteoblasts and osteoclasts in situ and in culture. J Cell Sci 103 (1992) 267-271.
• [4] Hughes D.E., Salter D.M., Dedhar S., Simpson R.: Integrin expression in human bone. J Bone Miner Res 8 (1993) 527-533.
• [5] Xiao G., Wang D., Benson M.D., Karsenty G., Franceschi R.T.: Role of the alpha2-integrin in osteoblast-specific gene expression and activation of the Osf2 transcription factor. J Biol Chem 273 (1998) 32988-32994.
• [6] Dedhar S.: Signal transduction via the beta 1 integrins is a required intermediate in interleukin-1 beta induction of alkaline phosphatase activity in human osteosarcoma cells. Exp Cell Res 183 (1989) 207-214.
• [7] Anselme K.: Osteoblast adhesion on biomaterials. Biomaterials 21 (2000) 667-681.
• [8] Cowles E.A., Brailey L.L., Gronowicz G.A.: Integrin-mediated signaling regulates AP-1 transcription factors and proliferation in osteoblasts. J Biomed Mater Res 52 (2000) 725-737.
• [9] Bennett J.H., Carter D.H., Alavi A.L., Beresford J.N., Walsh S.: Patterns of integrin expression in a human mandibular explant model of osteoblast differentiation. Arch Oral Biol 46 (2001) 229-238.
• [10] Nakamura I., Duong Le T., Rodan S.B., Rodan G.A.: Involvement of alpha(v)beta3 integrins in osteoclast function. J Bone Miner Metab 25 (2007) 337-344.
• [11] Raz P., Lohmann C.H., Turner J., Wang L., Poythress N., Blanchard C., et al: 1alpha,25(OH)2D3 regulation of integrin expression is substrate dependent. J Biomed Mater Res A 71 (2004) 217-225.
• [12] Lee M.H., Ducheyne P., Lynch L., Boettiger D., Composto R.J.: Effect of biomaterial surface properties on fibronectin-alpha5beta1 integrin interaction and cellular attachment. Biomaterials 27 (2006) 1907-1916.
• [13] Wan Y., Wang Y., Liu Z., Qu X., Han B., et al: Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured poly(L-lactide). Biomaterials 26 (2005) 4453-4459.
• [14] Zhang X., Hu J., Li Y., Yin G., Luo E.: Effects of ibandronate--hydroxyapatite on resorptive activity of osteoclasts. Arch Med Sci 7 (2011) 53-60.
• [15] Chin S.L., Johnson S.A., Quinn J., Mirosavljevic D., Price J.T., Dudley A.C., Thomas D.M.: A role for alphaV integrin subunit in TGF-beta-stimulated osteoclastogenesis. Biochem Biophys Res Commun 307 (2003) 1051-1058.
• [16] Pistone M., Sanguineti C., Federici A., Sanguineti F., Defilippi P., Santolini F., Querze G., et al: Integrin synthesis and utilization in cultured human osteoblasts. Cell Biol Int 20 (1996) 471-479.
• [17] Kudelska-Mazur D., Lewandowska-Szumiel M., Mazur M., Komender J.: Osteogenic cell contact with biomaterials influences phenotype expression. Cell Tissue Bank 6 (2005) 55-64.
• [18] Tohma Y., Tanaka Y., Ohgushi H., Kawate K., Taniguchi A., Hayashi K., Isomoto S., Takakura Y.: Early bone in-growth ability of alumina ceramic implants loaded with tissue-engineered bone. J Orthop Res 24 (2006) 595-603.
• [19] Burridge K., Fath K., Kelly T., Nuckolls G., Turner C.: Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu Rev Cell Biol 4 (1988) 487-525.
• [20] Schneider G.B., Whitson S.W., Cooper L.F.: Restricted and coordinated expression of beta3-integrin and bone sialoprotein during cultured osteoblast differentiation. Bone 24 (1999) 321-327.
• [21] Delannet M., Martin F., Bossy B., Cheresh D.A., Reichardt L.F., Duband J.L.: Specific roles of the alpha V beta 1, alpha V beta 3 and alpha V beta 5 integrins in avian neural crest cell adhesion and migration on vitronectin. Development 120 (1994) 2687-2702.
• [22] Wayner E.A., Orlando R.A., et al: Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface. J Cell Biol 113 (1991) 919-929.
• [23] Lai C.F., Cheng S.L.: Alphavbeta integrins play an essential role in BMP-2 induction of osteoblast differentiation. J Bone Miner Res 20 (2005) 330-340.
• [24] Maehata Y., Takamizawa S., Ozawa S., Kato Y., Sato S., Kubota E., Hata R.: Both direct and collagen-mediated signals are required for active vitamin D3-elicited differentiation of human osteoblastic cells: roles of osterix, an osteoblast-related transcription factor. Matrix Biol 25 (2006) 47-58.
• [25] Calvo M.S., Eyre D.R., Gundberg C.M.: Molecular basis and clinical application of biological markers of bone turnover. Endocr Rev 17 (1996) 333-368.
• [26] Amato I., Ciapetti G., Pagani S., Marletta G., Satriano C., Baldini N., Granchi D.: Expression of cell adhesion receptors in human osteoblasts cultured on biofunctionalized poly-(epsilon-caprolactone) surfaces. Biomaterials 28 (2007) 3668-3678.
• [27] Wang L., Zhao G., Olivares-Navarrete R., Bell B.F., Wieland M., Cochran D.L., Schwartz Z., Boyan B.D.: Integrin beta1 silencing in osteoblasts alters substrate-dependent responses to 1,25-dihydroxy vitamin D3. Biomaterials 27 (2006) 3716-3725.
• [28] Lewandowska-Szumiel M., Sikorski K., Szummer A., Komender J., et al.: Experimental model for observation of micromotion in cell culture. J Biomed Mater Res B Appl Biomater 72 (2005) 379-387.
• [29] Lewandowska-Szumiel M., Sikorski K., Szummer A., Lewandowski Z., Marczynski W.: Osteoblast response to the elastic strain of metallic support. J Biomech 40 (2007) 554-560.
• [30] Walboomers X.F., Jansen J.A.: Cell and tissue behavior on micro-grooved surfaces. Odontology 89 (2001) 2-11.
• [31] Takeuchi Y., Suzawa M., Kikuchi T., Nishida E., Fujita T., Matsumoto T.: Differentiation and transforming growth factor-beta receptor down-regulation by collagen-alpha2beta1 integrin interaction is mediated by focal adhesion kinase and its downstream signals in murine osteoblastic cells. J Biol Chem 272 (1997) 29309-29316.
• [32] Hirai F., Nakayamada S., Okada Y., Saito K., Kurose H., Mogami A., Tanaka Y.: Small GTPase Rho signaling is involved in beta1 integrin-mediated up-regulation of intercellular adhesion molecule 1 and receptor activator of nuclear factor kappaB ligand on osteoblasts and osteoclast maturation. Biochem Biophys Res Commun 356 (2007) 279-285.
• [33] Gronowicz G.A., Mccarthy M.B.: Glucocorticoids inhibit the attachment of osteoblasts to bone extracellular matrix proteins and decrease beta 1-integrin levels. Endocrinology 136 (1995) 598-608.
• [34] Siebers M.C., Walboomers X.F., Van Den Dolder J., Leeuwenburgh S.C., Wolke J.G., Jansen J.A.: The behavior of osteoblast-like cells on various substrates with functional blocking of integrin-beta1 and integrin-beta3. J Mater Sci Mater Med 19 (2008) 861-868.
• [35] Bertazzo S., Zambuzzi W.F., Da Silva H.A., Ferreira C.V., Bertran C.A.: Bioactivation of alumina by surface modification: a possibility for improving the applicability of alumina in bone and oral repair. Clin Oral Implants Res 20 (2009) 288-293.
• [36] Kim Y.H., Anirban J.M., Song H.Y., Seo H.S., Lee B.T.: In vitro and in vivo evaluations of 3D porous TCP-coated and non-coated alumina scaffolds. J Biomater Appl 25 (2011) 539-558.
• [37] Dedhar S., Argraves W.S., Suzuki S., Ruoslahti E., Pierschbacher M.D.: Human osteosarcoma cellsresistant to detachment by an Arg-Gly-Asp-containing peptide overproduce the fibronectin receptor. J Cell Biol 105 (1987) 1175-82.
• [38] Sinha R.K., Tuan R.S.: Regulation of human osteoblast integrin expression by orthopedic implant materials. Bone 18 (1996) 451-7.

Uwagi

EN

This work was supported by a grant (No. 3 T08A 001 30 for Edyta Wróbel) from Polish Ministry of Science and Higher Education. Biomaterials (pure alumina disc-shaped samples) were prepared by the Institute of Glass, Ceramics, Refractory and Construction Materials, 9 Postępu St., 02-676 Warszawa, Poland. Special thanks to Agnieszka Mikulska for preparing the primary cell cultures and Professor Małgorzata Lewandowska-Szumieł for valuable discussions and support.