Validated method to determine (±)-gossypol in Candida tropicalis culture by high-performance liquid chromatography

• Autorzy: Cheng C. , Cun-Xi N. , Jing L. , Yong-Qiang W. , Yan-Feng L. , Wen-Xia G. , Wen-Ju Z.

Identyfikatory

DOI

10.1556/1326.2018.00420

• Języki publikacji: EN

Abstrakty

EN

A validated high-performance liquid chromatography (HPLC) method has been developed to analyze the (±)-gossypol in the selection of strains of Candida tropicalis culture. Since gossypol was easily degraded and oxidized, the addition of antioxidant NADPH-Na4 and acetone extraction was chosen to prevent gossypol degradation and gradient elution assay was applied to obtain gossypol resolution. Concentrations of gossypol in C. tropicalis ZD-3 culture 20 μg/mL were determined, and concentration–time profiles were observed. Linearity of the gossypol standard curve by HPLC area method was ranged from 0.1 to 20 μg/mL with Y = 26.954 × X − 29.547, R₂ = 0.9991, and n = 3, with limit of detection (LOD) of 50 ng/mL and lower limit of quantification (LLOQ) of 500 ng/mL. The recovery rate is dose-dependent and ranged from 85.3% to 103.5%. It is a rapid and reliable HPLC method for gossypol quantization in microorganism culture which could be applied in solid fermentation in the feed industry.

Słowa kluczowe

EN

HPLC; gossypol; Candida tropicalis culture; acetone; gradient elution

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2018
• Tom: Vol. 30, no. 4
• Strony: 269--273
• Opis fizyczny: Bibliogr. 18 poz., rys.

Twórcy

autor

Cheng C.

• College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China

autor

Cun-Xi N.

• College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China

autor

Jing L.

• College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China

autor

Yong-Qiang W.

• College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China

autor

Yan-Feng L.

• College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China

autor

Wen-Xia G.

• College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China

autor

Wen-Ju Z.

• College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China

Bibliografia

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).