Validation of UV Spectrophotometric and HPLC Methods for Quantitative determination of chlorpyrifos

• Autorzy: Zalat O. A. , Elsayed M. A. , Fayed M. S. , Abd El Megid M. K.
• Języki publikacji: EN

Abstrakty

EN

A specific and sensitive HPLC and UV spectrophotometric methodwere developed for determination and analysis of chlorpyrifos. Chromatographic separation was achieved on a 150 mm x 10 mm I.D. reversed phase column Zorbax SB C-18. usingdeionizedwater: acetonitrile in the ratio of 10:90 v/v respectively as mobile phase. The effluent was monitored at 290 and 230 nm. Two sharp peaks were obtained for the solvent and chlorpyrifos at 2.7 and 3.45 min respectively. UV spectrophotometric method was performed at 290 nm using Isopropanol as the solvent. Linear range was 0.025-3500 ppm (r2 = 0.9986 ±0.0009) for HPLC method and 2.229 to 200 ppm (r2 = 0.9988) for UV spectrophotometric method. Validation guidelines and statistical analysis showed that both the methods were precise, accurate, sensitive, and can be used for the routine quality control of chlorpyrifos in waste discharges.

Słowa kluczowe

EN

chlorpyrifos; HPLC; UV-spectrophotometric; liner range

• Wydawca: Przedsiębiorstwo Wydawnictw Naukowych "DARWIN"
• Czasopismo: International Letters of Chemistry, Physics and Astronomy
• Rocznik: 2014
• Tom: Vol. 2
• Strony: 58--63
• Opis fizyczny: Bibliogr. 14 poz., rys.

Twórcy

autor

Zalat O. A.

• Department of Chemical Engineering, Military Technical College, Kobry Elkobbah, Cairo, Egypt

autor

Elsayed M. A.

• Department of Chemical Engineering, Military Technical College, Kobry Elkobbah, Cairo, Egypt

autor

Fayed M. S.

• Department of Chemical Engineering, Military Technical College, Kobry Elkobbah, Cairo, Egypt

autor

Abd El Megid M. K.

• Department of Chemical Engineering, Military Technical College, Kobry Elkobbah, Cairo, Egypt

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