Phytochemical, Anti-Inflammatory and Antioxidant Activities of Pistacia lentiscus L. Leaves from Ajdir, Al Hoceima Province, Morocco

• Autorzy: Labhar Amina , Benamari Omar , El-Mernissi Yahya , Salhi Amin , Ahari Mhamed , El Barkany Soufiane , Amhamdi Hassan

Identyfikatory

DOI

10.12912/27197050/169935

• Języki publikacji: EN

Abstrakty

EN

Pistacia lentiscus L. (PL) is a shrub belonging to the Anacardiaceae family, used in traditional medicine to treat various disorders in the Commune of Ajdir from Al Hociema province. The conducted study focused on determination of the polyphenols and flavonoids contents by spectrophotometric and in vitro evaluation of the antioxidant and anti-inflammatory capacity. Determining total polyphenols shows the aqueous extract with a higher concentration of 125.04±0.01 mg EAG/g ES, followed by the ethanolic extract of 108.16±0.02 mg EAG/g ES. Determination of flavonoids revealed that the hexanoic extract contains a maximum of flavonoids with a level of 90.60±0.01 mg EQ/g ES. The antioxidant activity of different extracts was determined through two methods: DPPH and FRAP. Aqueous and ethanolic extracts showed a high antioxidant capacity. Further, regarding anti-inflammatory activity, the ethanolic extract has good activity inhibition (92.65±0.67) followed by aqueous extract (94±0.29) at 1000 μg/mL concentration. This study found that the ethanolic extracts from PL leaves are a powerful natural antioxidant and effective anti-inflammatory agent. The results indicate the extract’s effectiveness and highlight the importance of medicinal plants from the Commune of Ajdir.

Słowa kluczowe

EN

Pistacia lentiscus; antioxidant activity; anti-inflammatory; polyphenols; medicinal plant; Al Hoceima; phytochemical

• Wydawca: Lubelski Oddział Polskiego Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology ; WNGB Wydawnictwo Naukowe
• Czasopismo: Ecological Engineering & Environmental Technology
• Rocznik: 2023
• Tom: Vol. 24, iss. 7
• Strony: 172--177
• Opis fizyczny: Bibliogr. 34 poz., tab.

Twórcy

autor

Labhar Amina

• Research Unit in Applied Chemistry (URCA), Faculty of Science and Technology, Abdelmalek Essaadi University, Al Hoceima, Morocco

• https://orcid.org/0000-0003-1015-484X

autor

Benamari Omar

• Research Unit in Applied Chemistry (URCA), Faculty of Science and Technology, Abdelmalek Essaadi University, Al Hoceima, Morocco

• https://orcid.org/0009-0004-0038-7919

autor

El-Mernissi Yahya

• Research Unit in Applied Chemistry (URCA), Faculty of Science and Technology, Abdelmalek Essaadi University, Al Hoceima, Morocco

• https://orcid.org/0000-0001-5807-8175

autor

Salhi Amin

• Research Unit in Applied Chemistry (URCA), Faculty of Science and Technology, Abdelmalek Essaadi University, Al Hoceima, Morocco

• https://orcid.org/0000-0002-5243-747X

autor

Ahari Mhamed

• Laboratory of Molecular Chemistry, Materials and Environment (LMCME), Department of Chemistry, Faculty Multidisciplinary Nador, Mohamed 1st University, P.B. 300, Nador 62700, Morocco

• https://orcid.org/0000-0002-0457-5923

autor

El Barkany Soufiane

• Laboratory of Molecular Chemistry, Materials and Environment (LMCME), Department of Chemistry, Faculty Multidisciplinary Nador, Mohamed 1st University, P.B. 300, Nador 62700, Morocco

• https://orcid.org/0000-0003-3756-2237

autor

Amhamdi Hassan

• Research Unit in Applied Chemistry (URCA), Faculty of Science and Technology, Abdelmalek Essaadi University, Al Hoceima, Morocco

• https://orcid.org/0000-0002-6693-5752

Bibliografia

• 1. Ait Mohand, B., El Antari, A., Benkhalti, F. 2020. Chemical composition of pistacia lentiscus seeds’ oil from Moroccan High Atlas Mountain. Journal of Food Quality, 2020. https://doi.org/10.1155/2020/5190491
• 2. Alam, M.N., Bristi, N.J., Rafiquzzaman, M. 2013. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21(2), 143–152. https://doi.org/10.1016/j.jsps.2012.05.002
• 3. Andreadou, I., Mitakou, S., Paraschos, S., Efentakis, P., Magiatis, P., Kaklamanis, L., Halabalaki, M., Skaltsounis, L., Iliodromitis, E.K. 2016. Pistacia lentiscus L. reduces the infarct size in normal fed anesthetized rabbits and possess antiatheromatic and hypolipidemic activity in cholesterol fed rabbits. Phytomedicine, 23(11), 1220–1226. https://doi.org/10.1016/j.phymed.2016.06.002
• 4. Aranega, A., Boulaiz, H. 2005. Cellular and molecular biology: Foreword. Cellular and Molecular Biology, 51(1), 1.
• 5. Arulselvan, P., Fard, M.T., Tan, W.S., Gothai, S., Fakurazi, S., Norhaizan, M.E., Kumar, S.S. 2016. Role of antioxidants and natural products in inflammation. Oxidative Medicine and Cellular Longevity, 2016. https://doi.org/10.1155/2016/5276130
• 6. Bampouli, A., Kyriakopoulou, K., Papaefstathiou, G., Louli, V., Aligiannis, N., Magoulas, K., Krokida, M. 2015. Evaluation of total antioxidant potential of Pistacia lentiscus var. chia leaves extracts using UHPLC-HRMS. Journal of Food Engineering, 167, 25–31. https://doi.org/10.1016/j.jfoodeng.2014.10.021
• 7. Barbouchi, M., Elamrani, K., El Idrissi, M., Choukrad, M. 2019. The effect of solvent extracts on the measurement of total polyphenol, flavonoid and tannin contents and its relation to antioxidant capacities from various parts of Terebinth (Pistacia terebinthus L.). Moroccan Journal of Chemistry, 7(2), 290–300.
• 8. Barbouchi, M., Elamrani, K., El Idrissi, M., Choukrad, M. 2020. A comparative study on phytochemical screening, quantification of phenolic contents and antioxidant properties of different solvent extracts from various parts of Pistacia lentiscus L. Journal of King Saud University - Science, 32(1), 302–306. https://doi.org/10.1016/j.jksus.2018.05.010
• 9. Boucheffa, S., Sobhi, W., Attoui, A., Selli, S., Kelebek, H., Semmeq, A., Benguerba, Y. 2021. Effect of the main constituents of Pistacia lentiscus leaves against the DPPH radical and xanthine oxidase : experimental and theoretical study radical and xanthine oxidase : experimental and theoretical study. Journal of Biomolecular Structure and Dynamics, 0(0), 1–15. https://doi.org/10.1080/07391102.2021.1936182
• 10. Boutemine, I.M., Amri, M., Amir, Z. C., Fitting, C., Mecherara-Idjeri, S., Layaida, K., Sennoun, N., Berkane, S., Cavaillon, J. M., Touil-Boukoffa, C. 2018. Gastro-protective, therapeutic and anti-inflammatory activities of Pistacia lentiscus L. fatty oil against ethanol-induced gastric ulcers in rats. Journal of Ethnopharmacology, 224(May), 273–282. https://doi.org/10.1016/j.jep.2018.05.040
• 11. Chaabani, E., Abert Vian, M., Bott, R., Ginies, C., Defoort, C., Ksouri, R., Chemat, F. 2020. Extraction of aromas from Pistacia lentiscus L. leaves using alternative solvents: COSMO-RS-assisted solvent screening and GC-MS metabolites profiling. Separation Science and Technology (Philadelphia), 55(4), 716–727. https://doi.org/10.1080/01496395.2019.1574821
• 12. Chen, J.Y., Yen, G.C., Tsai, N.T., Lin, J.A. 2021. Risk and benefit of natural and commercial dark brown sugars as evidenced by phenolic and maillard reaction product contents. Journal of Agricultural and Food Chemistry, 69(2), 767–775. https://doi.org/10.1021/acs.jafc.0c04795
• 13. Dellai, A., Souissi, H., Borgi, W., Bouraoui, A., Chouchane, N. 2013. Antiinflammatory and antiulcerogenic activities of Pistacia lentiscus L. leaves extracts. Industrial Crops and Products, 49, 879–882. https://doi.org/10.1016/j.indcrop.2013.07.010
• 14. Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P., Vidal, N. 2006. Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97(4), 654–660. https://doi.org/10.1016/j.foodchem.2005.04.028
• 15. Dragović, S., Dragović-Uzelac, V., Pedisić, S., Čošić, Z., Friščić, M., Garofulić, I.E., Zorić, Z. 2020. The mastic tree (Pistacia lentiscus l.) leaves as source of BACs: Effect of growing location, phenological stage and extraction solvent on phenolic content. Food Technology and Biotechnology, 58(3), 303–313. https://doi.org/10.17113/ftb.58.03.20.6662
• 16. El Bishbishy, M.H., Gad, H.A., Aborehab, N.M. 2020. Chemometric discrimination of three Pistacia species via their metabolic profiling and their possible in vitro effects on memory functions. Journal of Pharmaceutical and Biomedical Analysis, 177. https://doi.org/10.1016/j.jpba.2019.112840
• 17. Galanakis, C.M., Goulas, V., Tsakona, S., Manganaris, G.A., Gekas, V. 2013. A knowledge base for the recovery of natural phenols with different solvents. International Journal of Food Properties, 16(2), 382–396. https://doi.org/10.1080/10942912.2010.522750
• 18. Hadjichambis, A.C., Paraskeva-Hadjichambi, D., Della, A., Elena Giusti, M., De Pasquale, C., Lenzarini, C., Censorii, E., Reyes Gonzales-Tejero, M., Patricia Sanchez-Rojas, C., Ramiro-Gutierrez, J., Skoula, M., Johnson, C., Sarpaki, A., Hmamouchi, M., Jorhi, S., El-Demerdash, M., El-Zayat, M., Pieroni, A. 2008. Wild and semi-domesticated food plant consumption in seven circum-Mediterranean areas. International Journal of Food Sciences and Nutrition, 59(5), 383–414. https://doi.org/10.1080/09637480701566495
• 19. Huang, H., Hu, G., Wang, C., Xu, H., Chen, X., Qian, A. 2014. Cepharanthine, an alkaloid from Stephania cepharantha Hayata, inhibits the inflammatory response in the RAW264.7 cell and mouse models. Inflammation, 37(1), 235–246. https://doi.org/10.1007/s10753-013-9734-8
• 20. Huang, P.H., Cheng, Y.T., Chan, Y.J., Lu, W.C., Li, P.H. 2022. Effect of heat treatment on nutritional and chromatic properties of mung bean (Vigna radiata L.). Agronomy, 12(6), 1–15. https://doi.org/10.3390/agronomy12061365
• 21. Ladio, A., Lozada, M., Weigandt, M. 2007. Comparison of traditional wild plant knowledge between aboriginal communities inhabiting arid and forest environments in Patagonia, Argentina. Journal of Arid Environments, 69(4), 695–715. https://doi.org/10.1016/j.jaridenv.2006.11.008
• 22. Lekouaghet, A., Boutefnouchet, A., Bensuici, C., Gali, L., Ghenaiet, K., Tichati, L. 2020. In vitro evaluation of antioxidant and anti-inflammatory activities of the hydroalcoholic extract and its fractions from Leuzea conifera L. roots. South African Journal of Botany, 132, 103–107. https://doi.org/10.1016/j.sajb.2020.03.042
• 23. Leonti, M., Cabras, S., Weckerle, C.S., Solinas, M.N., Casu, L. 2010. The causal dependence of present plant knowledge on herbals-Contemporary medicinal plant use in Campania (Italy) compared to Matthioli 1568. Journal of Ethnopharmacology, 130(2), 379–391. https://doi.org/10.1016/j.jep.2010.05.021
• 24. Ljubuncic, P., Song, H., Cogan, U., Azaizeh, H., Bomzon, A. 2005. The effects of aqueous extracts prepared from the leaves of Pistacia lentiscus in experimental liver disease. Journal of Ethnopharmacology, 100(1–2), 198–204. https://doi.org/10.1016/J.JEP.2005.03.006
• 25. Milia, E., Bullitta, S.M., Mastandrea, G., Szotáková, B., Schoubben, A., Langhansová, L., Quartu, M., Bortone, A., Eick, S. 2021. Leaves and fruits preparations of Pistacia lentiscus L.: A review on the ethnopharmacological uses and implications in inflammation and infection. Antibiotics, 10(4), 425. https://doi.org/10.3390/ANTIBIOTICS10040425
• 26. Milia, E., Usai, M., Szotáková, B., Elstnerová, M., Králová, V., D’hallewin, G., Spissu, Y., Barberis, A., Marchetti, M., Bortone, A., Campanella, V., Mastandrea, G., Langhansová, L., Eick, S. 2020. The pharmaceutical ability of Pistacia lentiscus L. leaves essential oil against periodontal bacteria and Candida sp. and its anti-inflammatory potential. Antibiotics, 9(6). https://doi.org/10.3390/antibiotics9060281
• 27. Nocentini, S., Travaglini, D., Muys, B. 2022. Managing mediterranean forests for multiple ecosystem services: Research progress and knowledge gaps.Current Forestry Reports, 8(2), 229–256. https://doi.org/10.1007/s40725-022-00167-w
• 28. Pachi, V.K., Mikropoulou, E.V., Gkiouvetidis, P., Siafakas, K., Argyropoulou, A., Angelis, A., Mitakou, S., Halabalaki, M. 2020. Traditional uses, phytochemistry and pharmacology of Chios mastic gum (Pistacia lentiscus var. Chia, Anacardiaceae): A review. Journal of Ethnopharmacology, 254. Elsevier Ireland Ltd. https://doi.org/10.1016/j.jep.2019.112485
• 29. Salhi, A., Bellaouchi, R., Barkany, S. El, Rokni, Y., Bouyanzer, A., Asehraou, A., Amhamdi, H., Zarrouk, A., Hammouti, B. 2019. Total phenolic content, antioxidant and antimicrobial activities of extracts from Pistacia lentiscus leaves. Caspian Journal of Environmental Sciences, 17(3), 189–198. https://doi.org/10.22124/cjes.2019.3662
• 30. Sehaki, C., Jullian, N., Ayati, F., Fernane, F., Gontier, E. 2023. A review of Pistacia lentiscus polyphenols: Chemical diversity and pharmacological activities. Plants, 12(2). https://doi.org/10.3390/plants12020279
• 31. Trabelsi, H., Cherif, O. A., Sakouhi, F., Villeneuve, P., Renaud, J., Barouh, N., Boukhchina, S., Mayer, P. 2012. Total lipid content, fatty acids and 4-des-methylsterols accumulation in developing fruit of Pistacia lentiscus L. growing wild in Tunisia. Food Chemistry, 131(2), 434–440. https://doi.org/10.1016/J.FOODCHEM.2011.08.083
• 32. Velioglu, Y.S., Mazza, G., Gao, L., Oomah, B.D. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry, 46(10), 4113–4117. https://doi.org/10.1021/jf9801973
• 33. Vogiatzoglou, A., Mulligan, A.A., Lentjes, M.A.H., Luben, R.N., Spencer, J.P.E., Schroeter, H., Khaw, K.T., Kuhnle, G.G.C. 2015. Flavonoid intake in european adults (18 to 64 years). Plos One, 10(5), e0128132. https://doi.org/10.1371/JOURNAL.PONE.0128132
• 34. Zitouni, A., Belyagoubi Benhammou, N., Ghembaza, N., Toul, F., Atik Bekkara, F. 2016. Assessment of phytochemical composition and antioxidant properties of extracts from the leaf, stem, fruit and root of Pistacia lentiscus L. International Journal of Pharmacognosy and Phytochemical Research, 8(4), 627–633.