Synergistic effect of selenium and genipin triggers viability of 3T3 cells on PVA/Gelatin scaffolds

• Autorzy: Erdag Demet , Koc Serkan N. , Oksuzomer M. Faruk , Yalcintepe Leman

Identyfikatory

DOI

10.37190/ABB-01944-2021-04

• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to reveal the first time synergistic effect of GP and selenium (Se) on 3T3 cells seeded on natural and non-cytotoxic porous scaffolds with poly(vinyl alcohol) (PVA) and gelatin (GE). Methods: Electrospinning scaffolds were produced as PVA/GE/GA crosslinked with glutaraldehyde (GA) and freeze/dried scaffolds crosslinked with genipin (GP) were divided into two groups as PVA/GE/GP5 and PVA/GE/GP8. The scaffolds were investigated in terms of pore morphology, swell ratio, biodegradation, and biocompatibility. The biocompatibility of the material was tested in vitro by MTT assay on 1, 2, and 3 days to test the cell viability of 3T3 cells. Results: It was observed that Se triggered the excellent cell growth and proliferation on electrospinning and freeze drying PVA/GE scaffolds. Conclusions: Selenium with PVA/GE scaffolds can be a promising candidate for wound healing application, as it significantly increases cell viability on scaffolds. It is thought that the synergistic effect of selenium with genipin may be an important step in tissue engineering applications. The preliminary study can be supported by in vivo studies in the future.

Słowa kluczowe

EN

gelatin; genipin; 3T3 cells; scaffold; selenium

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2022
• Tom: Vol. 24, no. 1
• Strony: 179--190
• Opis fizyczny: Bibliogr. 45 poz., rys., wykr.

Twórcy

autor

Erdag Demet

• Department of Biophysics, Istanbul University, Istanbul Faculty of Medicine, Fatih, Istanbul, Turkey.
• Department of Computer Technologies, Biruni University, Vocational School, Topkapi, Istanbul, Turkey.

autor

Koc Serkan N.

• Department of Chemical Engineering, Istanbul University, Cerrahpasa, Chemical Engineering, Avcilar, Istanbul, Turkey.

autor

Oksuzomer M. Faruk

• Department of Chemical Engineering, Istanbul University, Cerrahpasa, Chemical Engineering, Avcilar, Istanbul, Turkey.

autor

Yalcintepe Leman

• Department of Biophysics, Istanbul University, Istanbul Faculty of Medicine, Fatih, Istanbul, Turkey.

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