Distribution of antibiotic resistance and the presence of vancomycin-resistance genes (vanA and vanB) in Enterobacteriaceae isolated from the Sea of Marmara, the Canakkale Strait and the Istanbul Strait, Turkey

• Autorzy: Çardak M. , Altuğ G. , Ayad M. , Erol Ő.

Identyfikatory

DOI

10.1515/ohs-2016-0017

• Języki publikacji: EN

Abstrakty

EN

We investigated the frequency of antibiotic resistance of Enterobacteriaceae and the presence of vancomycinresistance genes in samples taken from the Sea of Marmara, and the Istanbul and Canakkale Straits, Turkey. Different colony-forming bacteria were isolated and identiffed with the VITEK 2 Compact 30 system. The antibiotic resistance of the isolates was determined by the disc diffusion method. The isolates were tested against amoxicillin, ampicillin, aztreonam, ce azidime, cefotaxime, cefuroxime, o oxacin, vancomycin, tetracycline, kanamycin and gentamycin. e presence of vancomycin-resistance genes (vanA and vanB) was also investigated. the level of Enterobacteriaceae species was higher in the Sea of Marmara than in the Istanbul Strait and the Canakkale Strait. Isolates showing resistance to the greatest number of antibiotics were identiffed from E. coli isolates. the resistance of the selected bacterial isolates were as follows: kanamycin (82%), vancomycin (78%) and ampicillin (60%). Some intermediately vancomycin-resistant Enterobacteriaceae isolates had the vanA gene. This study provides evidence of widespread bacterial resistance to clinically relevant antibiotics in marine environments. It also contributes to the knowledge on the distribution of antibiotic resistance among Enterobacteriaceae and indicates the importance of control measures in domestic water treatment.

Słowa kluczowe

EN

Canakkale Strait; Istanbul Strait; Sea of Marmara; Beta-lactam Antibiotics; PCR; vanA; vanB

• Wydawca: Institute of Oceanography University of Gdańsk
• Czasopismo: Oceanological and Hydrobiological Studies
• Rocznik: 2016
• Tom: Vol. 45, No. 2
• Strony: 182--190
• Opis fizyczny: Bibliogr. 36 poz., rys., tab., wykr.

Twórcy

autor

Çardak M.

• Canakkale Onsekiz Mart University, Faculty of Marine Science and Technology, Canakkale, Turkey

autor

Altuğ G.

• Istanbul University, Faculty of Fisheries, Istanbul, Turkey

autor

Ayad M.

• Canakkale Onsekiz Mart University, Natural and Applied Sciences, Canakkale, Turkey

autor

Erol Ő.

• Canakkale Onsekiz Mart University, Natural and Applied Sciences, Canakkale, Turkey

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Uwagi

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.