Monitorowanie metabolizmu komórkowego za pomocą metody spektroskopii NMR

• Autorzy: Pudełko-Malik Natalia , Sapeta Monika , Młynarz Piotr

Identyfikatory

DOI

10.53584/wiadchem.2022.5.4

Warianty tytułu

EN

Monitoring of cellular metabolism by NMR spectroskopy

• Języki publikacji: PL

Abstrakty

EN

Metabolomics approaches allow systematic identification and quantitation of all metabolites in biological samples analyzes. As already known metabolism is directly or indirectly related to every aspect of cell function, therefore a careful observation of every changes taking place in metabolism, for example endogenous biochemical reaction products, reflectsthe phenotype of any living cell. Monitoring the metabolite profiles using metabolomics technologies, especially nuclear magnetic resonance (NMR) spectroscopy based on cell culture, allows us to evaluate drug efficiency and outcome of experimental therapy, and most importantly, it allows us to monitor the reaction of the model cell lines to a given treatment. The continued development of metabolomic approaches, e.g. analytical technique, or chemometric software, will accelerate the widespread use of metabolomics not only in the clinical field but also in different biological fields. This work presents a use of nuclear magnetic resonance to characterize and understand the cellular metabolome in a wide range of pathophysiological and clinical contexts.

Słowa kluczowe

PL

metabolomika; NMR; linia komórkowa

EN

metabolomics; nuclear magnetic resonance (NMR); cell line

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Wiadomości Chemiczne
• Rocznik: 2022
• Tom: [Z] 76, 5-6
• Strony: 323--333
• Opis fizyczny: Bibliogr. 25 poz., rys.

Twórcy

autor

Pudełko-Malik Natalia

• studentka Katedry Biochemii, Biologii Molekularnej i Biotechnologii, Wydział Chemiczny, Politechnika Wrocławska, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław

• https://orcid.org/0000-0002-0250-3029

autor

Sapeta Monika

• studentka Katedry Biochemii, Biologii Molekularnej i Biotechnologii, Wydział Chemiczny, Politechnika Wrocławska, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław

• https://orcid.org/0000-0002-4177-3398

autor

Młynarz Piotr

• Katedra Biochemii, Biologii Molekularnej i Biotechnologii, Wydział Chemiczny, Politechnika Wrocławska, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław

• https://orcid.org/0000-0002-8502-4022

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