Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy

• Autorzy: Kubicz E. , Jasińska B. , Zgardzińska B. , Bednarski T. , Białas P. , Czerwiński E. , Gajos A. , Gorgol M. , Kamińska D. , Kapłon Ł. , Kochanowski A. , Korcyl G. , Kowalski P. , Kozik T. , Krzemień W. , Niedźwiecki S. , Pałka M. , Raczyński L. , Rajfur Z. , Rudy Z. , Rundel O. , Sharma N. G. , Silarski M. , Słomski A. , Strzelecki A. , Wieczorek A. , Wiślicki W. , Zieliński M. , Moskal P.

Identyfikatory

DOI

10.1515/nuka-2015-0135

• Konferencja: Polish Seminar on Positron Annihilation (42 nd ; 29.06-01.07.2016 ; Lublin, Poland)
• Języki publikacji: EN

Abstrakty

EN

Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images.

Słowa kluczowe

EN

environmental scanning electron microscopy (ESEM); free volume; inverted microscopy; positron annihilation; positronium; yeasts

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2015
• Tom: Vol. 60, No. 4, part 1
• Strony: 749--753
• Opis fizyczny: Bibliogr. 25 poz., rys.

Twórcy

autor

Kubicz E.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Jasińska B.

• Department of Nuclear Methods, Institute of Physics, Maria Curie-Sklodowska University, 1 M. Curie-Sklodowskiej Sq., 20-031 Lublin, Poland

autor

Zgardzińska B.

• Department of Nuclear Methods, Institute of Physics, Maria Curie-Sklodowska University, 1 M. Curie-Sklodowskiej Sq., 20-031 Lublin, Poland

autor

Bednarski T.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Białas P.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Czerwiński E.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Gajos A.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Gorgol M.

• Department of Nuclear Methods, Institute of Physics, Maria Curie-Sklodowska University, 1 M. Curie-Sklodowskiej Sq., 20-031 Lublin, Poland

autor

Kamińska D.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Kapłon Ł.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
• Institute of Metallurgy and Materials Science PAS, 25 W. Reymonta Str., 30-059 Kraków, Poland

autor

Kochanowski A.

• Faculty of Chemistry, Department of Chemical Technology, Jagiellonian University, 3 R. Ingardena Str., 30-059 Kraków, Poland

autor

Korcyl G.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Kowalski P.

• Świerk Computing Centre, National Centre for Nuclear Research, 7 Andrzeja Soltana Str., 05-400 Otwock/Świerk, Poland

autor

Kozik T.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Krzemień W.

• High Energy Physics Division, National Centre for Nuclear Research, 7 Andrzeja Soltana Str., 05-400 Otwock/Świerk, Poland

autor

Niedźwiecki S.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Pałka M.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Raczyński L.

• Świerk Computing Centre, National Centre for Nuclear Research, 7 Andrzeja Soltana Str., 05-400 Otwock/Świerk, Poland

autor

Rajfur Z.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Rudy Z.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Rundel O.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Sharma N. G.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Silarski M.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Słomski A.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Strzelecki A.

autor

Wieczorek A.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
• Institute of Metallurgy and Materials Science PAS, 25 W. Reymonta Str., 30-059 Kraków, Poland

autor

Wiślicki W.

• Świerk Computing Centre, National Centre for Nuclear Research, 7 Andrzeja Soltana Str., 05-400 Otwock/Świerk, Poland

autor

Zieliński M.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

autor

Moskal P.

• Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland

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