Quantification of retinyl palmitate, thiamine, niacin, pyridoxine, folic acid, cyanocobalamin, zinc, and iron by chromatographic methods in fortified kernels and fortified rice

• Autorzy: Ivarsen Elise , Andersen Christoffer P. , Jensen Sabine M. , Pedersen Carsten T. , Svaneborg Anders K.

Identyfikatory

DOI

10.1556/1326.2020.00770

• Języki publikacji: EN

Abstrakty

EN

This study presents the optimization and validation of methods for the analysis of retinol, thiamine, niacin, pyridoxine, folic acid, cyanocobalamin, zinc, and iron in fortified kernels (coated and extruded) and in fortified rice. The analyses were performed by HPLC-UV/FLD/MS and ICP-OES. The optimized methods showed good resolution of the analyte peaks, excellent recovery (87–108%), reproducibility with relative standard deviation (SD) of analyte content between 1.8 and 11% and high correlation coefficient of the calibration curves (R2 > 0.997). Limit of detection was from 2.8 E-4 mg/kg for pyridoxine to 1.26 mg/kg for zinc and limit of quantification was from 9.2 E-4 mg/kg for pyridoxine to 4.21 mg/kg for zinc. Thereby the optimized methods demonstrated reliability and sensitivity in the detection and quantification of these micronutrients and that they are suitable for routine analysis of fortified kernels (coated and extruded) and fortified rice.

Słowa kluczowe

EN

vitamins; minerals; rice; HPLC; ICP-OES

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2021
• Tom: Vol. 33, no. 2
• Strony: 145--152
• Opis fizyczny: Bibliogr. 16 poz., rys., tab.

Twórcy

autor

Ivarsen Elise

• Eurofins Vitamin Testing Denmark, Ladelundvej 85, Vejen, 6600, Denmark

• https://orcid.org/0000-0003-3174-1808

autor

Andersen Christoffer P.

• Eurofins Vitamin Testing Denmark, Ladelundvej 85, Vejen, 6600, Denmark

autor

Jensen Sabine M.

• Eurofins Vitamin Testing Denmark, Ladelundvej 85, Vejen, 6600, Denmark

autor

Pedersen Carsten T.

• Eurofins Vitamin Testing Denmark, Ladelundvej 85, Vejen, 6600, Denmark

autor

Svaneborg Anders K.

• Eurofins Environment A/S, Ladelundvej 85, Vejen, 6600, Denmark

Bibliografia

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• 2. Guideline: fortification of rice with vitamins and minerals as a public health strategy; World Health Organization: Geneva 2018. Licence: CC BY-NC-SA 3.0 IGO.
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• 7. BS EN14122: Foodstuffs. Determination of vitamin B1 by high performance liquid chromatography; CEN-TC 275 WG 9, 2014.
• 8. BS EN15562: Foodstuffs. Determination of niacin by HPLC; CENTC 275 WG 9, 2009.
• 9. BS EN 14164: Foodstuffs. Determination of vitamin B6 by high performance chromatography; CEN-TC 275 WG 9, 2014.
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• 12. Meisser-Redeuil, K.; Bénet, S.; Gimez, C.; Campos-Giménez, E.; Nelson, M. J. AOAC Int. 2014, 97(4)1121–1126.
• 13. OECD. Safety Assessment of Foods and Feeds Drived from Transgenic Crops; Novel food and feed safety, OECD Publishing: Paris, 2015; Vol. 1, pp P141–142.
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• 16. Guidance for standard method performance requirements, AOAC SMPR, draft version 12.1, 31-jan-11.

Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2021).