Quality evaluation of Yanghuo Sanqi tablet through a simultaneous determination of five major active Flavonoids and three main saponins by HPLC-DAD-ELSD

• Autorzy: Zhu F. X , Jia X. B , Cai Y. , Chen Y.
• Języki publikacji: EN

Abstrakty

EN

Yanghuo Sanqi tablet (YST), combined prescription mainly derived from the leaves of Herba epimedii and the roots of Panax notoginseng, is a traditional Chinese medicine (TCM). Flavonoids (icarrin, epimedin A, epimedin B, epimedin C, and baohuoside I) and saponins (notoginsenoside R1, ginsenoside Rgl, and ginsenoside Rbl) are considered as the main bioactive compounds of YST. However, there is no report on quality control of TCMs by simultaneous determination of above-mentioned flavonoids and saponins so far. In this work, for the first time, a high-performance liquid chromatography-diode array detector-evaporative light scattering detector (HPLC-DAD-ELSD) method was developed to evaluate the quality of YST through a simultaneous determination of five major active flavonoids and three main saponins. Optimum separations were obtained with a Zorbax SB-C18 column by gradient elution with acetonitrile-water as the mobile phase. The drift tube temperature of ELSD was set at 105 °C, and the nebulizing gas flow rate was 2.5 L min^-1. The fully validated method was successfully applied to quantify the eight bioactive components in three lot products. This simple, low-cost, and reliable HPLC-DAD-ELSD method provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components.

Słowa kluczowe

EN

Yanghuo Sanqi; quality evaluation; flavonoid; saponin; icarrin; ginsenoside; HPLC-DAD-ELSD

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2012
• Tom: Vol. 24, no. 4
• Strony: 667--677
• Opis fizyczny: Bibliogr. 24 poz., rys., tab.

Twórcy

autor

Zhu F. X

• Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China

autor

Jia X. B

• Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China

autor

Cai Y.

• Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China

autor

Chen Y.

• Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China

Bibliografia

• [1] T.H. Xue and R. Roy, Science, 300, 740–741 (2003)
• [2] D. Normile, Science, 299, 188–190 (2003)
• [3] X. Xiao, Z. Wang, and Y. Lou, Chin Traditional Herbal Drugs, 37, 888–891 (2006)
• [4] H.Z. Zheng, Z.H. Dong, and Q. She, In Modern Research and Application of Traditional Chinese Medicine, Xueyuan Press, Beijing, China, 1998, pp. 4225
• [5] J.P. Xie and W.J. Sun, Strait Pharm. J., 18, 17–20 (2006)
• [6] G. Liu, J.C. Liu, Y.L. Zheng, and C.X. Zhang, Renshen Yanjiu, 2, 10–18 (2004)
• [7] D. Sha, Y. Chen, M. Zhang, and W. Yang, Chin. J. Pharm. Anal., 28, 404–407 (2008)
• [8] X.J. Chen, B.L. Guo, S.P. Li, Q.W. Zhang, P.F. Tu, and Y.T. Wang, J. Chromatogr. A, 1163, 96–104 (2007)
• [9] X.J. Chen, H. Ji, Q.W. Zhang, P.F. Tu, Y.T. Wang, B.L. Guo, and S.P. Li, J. Pharm. Biomed. Anal., 46, 226–235 (2008)
• [10] W. Li and J.F. Fitzloff, J. Pharm. Pharmacol., 53, 1637–1643 (2001)
• [11] D.X. Sha and M.L. Zhang, China J. Chin. Mater. Med., 30, 112–115 (2005)
• [12] J.B. Wan, F.Q. Yang, S.P. Li, Y.T. Wang, and X.M. Cui, J. Pharm. Biomed. Anal., 41, 1596–1601 (2006)
• [13] Y.Z. Huang and N.S. Wang, Trad. Chin. Drug Res. Clin. Pharmacol., 13, 174–177 (2002)
• [14] C.Y. Chen, L.W. Qi, H.J. Li, P. Li, L. Yi, H.L. Ma, and D. Tang, J. Sep. Sci., 30, 3181–3192 (2007)
• [15] L.W. Qi, Q.T. Yu, L. Yi, M.T. Ren, X.D. Wen, Y.X. Wang, and P. Li, J. Sep. Sci., 31, 97–106 (2008)
• [16] L. Yi, L.W. Qi, P. Li, Y.H. Ma, Y.J. Luo, and H.Y. Li, Anal. Bioanal. Chem., 389, 571–580 (2007)
• [17] Q.T. Yu, L.W. Qi, P. Li, L. Yi, J. Zhao, and Z. Bi, J. Sep. Sci., 30, 1292–1299 (2007)
• [18] Y.J. Wei, L.W. Qi, P. Li, H.W. Luo, Y. Ling, and L.H. Sheng, J. Pharm. Biomed. Anal., 45, 775–784 (2007)
• [19] J. Li, H. Qi, L.W. Qi, L. Yi, and P. Li, Anal. Chim. Acta., 596, 264–272 (2007)
• [20] J. Zhao, D. Wang, S. Duan, J. Wang, J. Bai, and W. Li, Arch. Pharm. Res., 32, 989–996 (2009)
• [21] J. Ye, X. Zhang, W. Dai, S. Yan, H. Huang, X. Liang, Y. Li, and W. Zhang, J. Pharm. Biomed. Anal., 49, 638–645 (2009)
• [22] Y. Lu, K. Yu, H.B. Qu, and Y.Y. Cheng, Chromatographia, 65, 19–24 (2007)
• [23] Y.F. Li, Y. Wang, H.B. Qu, and Y.Y. Cheng, Chromatographia, 67, 293–297 (2008)
• [24] H.R. Liang, W.M. Yan, L. Li, L. Jiang, and L. Dai, Chin. J. Chin. Mater. Med., 18, 677–679 (1993)