No evidence of the long-term in vitro toxicity of Aeroxide P25 TiO2 nanoparticles in three mammalian cell lines despite the initial reduction of cellular mitochondrial activity

• Autorzy: Męczyńska-Wielgosz Sylwia , Bartłomiejczyk Teresa , Grądzka Iwona , Sommer Sylwester , Węgierek-Ciuk Aneta , Lankoff Anna , Sikorska Katarzyna , Wojewódzka Maria , Dobrzyńska Małgorzata , Kruszewski Marcin

Identyfikatory

DOI

10.2478/nuka-2024-0002

• Języki publikacji: EN

Abstrakty

EN

We studied the effects of Aeroxide P25 titanium dioxide nanoparticles (TiO2 NPs) with a diameter of 21 nm on induction of DNA damage and long-term survival of three human cell lines: hepatocellular liver carcinoma HepG2, colorectal adenocarcinoma HT29 and lung carcinoma A549. The endpoints examined were DNA breakage estimated by the comet assay and oxidative base damage recognized by formamide-pyrimidine glycosylase (FPG) estimated with the FPG+ comet assay, frequencies of histone H2AX foci and micronuclei, apoptosis, cell metabolic activity measured by mitochondrial activity (MTT) assay and long-term survival measured by colony-forming ability. Each cell line had a different pattern of DNA breakage and base damage vs. nanoparticle (NP) concentration and treatment time. There was no increase in the frequencies of histone H2AX foci and micronuclei as compared to those in the untreated cells. In parallel with these results, no induction of apoptosis has been found in none of the cell lines tested. The reported experiments provided no evidence of the long-term in vitro toxicity of Aeroxide P25 TiO2 NPs, despite a slight decrease in mitochondrial activity and cell survival during the first 72 h.

Słowa kluczowe

EN

γH2AX assay; caspase 3 activity; comet assay; micronucleus assay; MTT assay; apoptosis

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2024
• Tom: Vol. 69, No. 1
• Strony: 13--22
• Opis fizyczny: Bibliogr. 28 poz., rys.

Twórcy

autor

Męczyńska-Wielgosz Sylwia

• Centre for Radiobiology and Biological Dosimetry Institute of Nuclear Chemistry and Technology Dorodna 16 St., 03-195 Warszawa, Poland

• https://orcid.org/0000-0002-++9475-983X+

autor

Bartłomiejczyk Teresa

• Centre for Radiobiology and Biological Dosimetry Institute of Nuclear Chemistry and Technology Dorodna 16 St., 03-195 Warszawa, Poland

autor

Grądzka Iwona

• Centre for Radiobiology and Biological Dosimetry Institute of Nuclear Chemistry and Technology Dorodna 16 St., 03-195 Warszawa, Poland

autor

Sommer Sylwester

• Centre for Radiobiology and Biological Dosimetry Institute of Nuclear Chemistry and Technology Dorodna 16 St., 03-195 Warszawa, Poland

• https://orcid.org/0000-0001-8405-5460

autor

Węgierek-Ciuk Aneta

• Institute of Biology, Jan Kochanowski University Kielce, Poland

• https://orcid.org/0000-0002-6527-7364

autor

Lankoff Anna

• Institute of Nuclear Chemistry and Technology Warszawa, Poland
• Institute of Biology, Jan Kochanowski University Kielce, Poland

• https://orcid.org/0000-0002-6661-7949

autor

Sikorska Katarzyna

• Centre for Radiobiology and Biological Dosimetry Institute of Nuclear Chemistry and Technology Dorodna 16 St., 03-195 Warszawa, Poland

• https://orcid.org/0000-0001-8480-7722

autor

Wojewódzka Maria

• Centre for Radiobiology and Biological Dosimetry Institute of Nuclear Chemistry and Technology Dorodna 16 St., 03-195 Warszawa, Poland

• https://orcid.org/0000-0003-0809-4614

autor

Dobrzyńska Małgorzata

• Department of Radiation Hygiene and Radiobiology National Institute of Public Health – National Institute of Hygiene, Warszawa, Poland

• https://orcid.org/0000-0002-2185-3745

autor

Kruszewski Marcin

• Centre for Radiobiology and Biological Dosimetry Institute of Nuclear Chemistry and Technology Warszawa, Poland and Department of Molecular Biology and Translational Research, Institute of Rural Health, Lublin, Poland

• https://orcid.org/0000-0003-4554-8566

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2024).