Determination of licochalcone A in rat plasma by UPLC–MS/MS and its pharmacokinetics

• Autorzy: Weng Qinghua , Chen Lianguo , Ye Luxin , Lu Xiaojie , Yu Zheng , Wen Congcong , Chen R. , Huang Gang

Identyfikatory

DOI

10.1556/1326.2018.00491

• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to establish a rapid, sensitive, and selective ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method to quantify the concentrations of licochalcone A and applicate the technique to its pharmacokinetic study. Analytes were separated on an UPLC ethylene bridged hybrid (BEH) C18 column (2.1 mm × 50 mm, 1.7 μm). The mobile phase was consisted of acetontrile and 0.1% formic acid with a flow rate of 0.4 mL/min in a gradient elution mode. Multiple-reaction monitoring (MRM) was carried out in a negative mode for licochalcone A (m/z 337.2 → 119.7) and the internal standard (IS) (m/z 609.0 → 300.9). The linearity of licochalcone A was great from 0.53 to 530 ng/mL. The lower limit of quantification and the lower limit of detection were 0.53 ng/mL and 0.26 ng/mL, respectively. The intra-day precision was less than 14%, and the inter-day precision was no more than 11%. The accuracy was from 91.5% to 113.9%, the recovery was over 90.5%, and the matrix effect was between 84.5% and 89.7%. The results of stability were in an acceptable range. The bioavailability was only 3.3%, exhibiting poor absorption. The developed method was successfully applicable for determining the concentrations of licochalcone A and its pharmacokinetic study.

Słowa kluczowe

EN

UPLC–MS/MS; licochalcone A; pharmacokinetics; bioavailability

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2019
• Tom: Vol. 31, no. 4
• Strony: 262--265
• Opis fizyczny: Bibliogr. 37 poz., rys., tab.

Twórcy

autor

Weng Qinghua

• The Third Clinical Institute Affiliated to Wenzhou Medical University & Wenzhou People's Hospital, Wenzhou 325000, China

autor

Chen Lianguo

• The Third Clinical Institute Affiliated to Wenzhou Medical University & Wenzhou People's Hospital, Wenzhou 325000, China

autor

Ye Luxin

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China

autor

Lu Xiaojie

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China

autor

Yu Zheng

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China

autor

Wen Congcong

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China

autor

Chen R.

• The Third Clinical Institute Affiliated to Wenzhou Medical University & Wenzhou People's Hospital, Wenzhou 325000, China

autor

Huang Gang

• Department of Traditional Chinese Medicine, The Sixth Affiliated Hospital of Wenzhou Medical University, The People's Hospital of Lishui, Lishui 323000, China

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).