Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase

• Autorzy: Douglas T. E. L. , Skwarczyńska A. , Modrzejewska Z. , Balcaen L. , Schaubroeck D. , Lycke S. , Vanhaecke F. , Vandenabeele P. , Dubruel P. , Jansen J. A. , Leeuwenburgh S. C. G.
• Języki publikacji: EN

Abstrakty

EN

Thermosensitive chitosan hydrogels containing sodium beta-glycerophosphate (ß-GP), whose gelation is induced by increasing temperature to body temperature, were functionalized by incorporation of Alkaline Phosphatase (ALP), an enzyme involved in mineralization of bone. ALP incorporation led to acceleration of gelation upon increase of temperature for four different chitosan preparations of differing molecular weight, as demonstrated by rheometric time sweeps at 37°C. Hydrogels containing ALP were subsequently incubated in calcium glycerophosphate (Ca-GP) solution to induce their mineralization with calcium phosphate (CaP) in order to improve their suitability as materials for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP mineral, as confirmed by SEM, FTIR, Raman spectroscopy, XRD, ICP-OES, and increases in dry mass percentage, which rose with increasing ALP concentration and incubation time in Ca-GP solution. The results demonstrate that ALP accelerates formation of thermosensitive chitosan/ß-GP hydrogels and induces their mineralization with CaP, which paves the way for applications as injectable bone replacement materials.

Słowa kluczowe

EN

chitosan; thermosensitivity; composite; mineralization

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2012
• Tom: Vol. 15, no. 116-117 spec. iss.
• Strony: 135--136
• Opis fizyczny: Bibliogr. 3 poz., rys., wykr.

Twórcy

autor

Douglas T. E. L.

• Department of Biomaterials, Radboud University Medical Center Nijmegen, The Netherlands
• Polymer Chemistry and Biomaterials (PBM) Group, Ghent University, Krijgslaan 281 S4, 9000 Gent, Belgium

autor

Skwarczyńska A.

• Department of Environmental Systems Engineering, Faculty of Process and Environmental Engineering, Technical University of Łódź, 213 Wolczanska Str., 90-924, Łódź, Poland

autor

Modrzejewska Z.

• Department of Environmental Systems Engineering, Faculty of Process and Environmental Engineering, Technical University of Łódź, 213 Wolczanska Str., 90-924, Łódź, Poland

autor

Balcaen L.

• Department of Analytical Chemistry, Ghent University, Krijgslaan 281 S12, 9000 Ghent, Belgium

autor

Schaubroeck D.

• Center for Microsystems Technology (CMST), ELIS, imec, Technologiepark 914a, 9052 Ghent, Belgium

autor

Lycke S.

• Raman Spectroscopy Research Group, Ghent University, Krijgslaan 281 S12, 9000 Ghent, Belgium

autor

Vanhaecke F.

• Department of Analytical Chemistry, Ghent University, Krijgslaan 281 S12, 9000 Ghent, Belgium

autor

Vandenabeele P.

• Department of Archaeology, Ghent University, Sint-Pietersnieuwstraat 35, 9000 Ghent, Belgium

autor

Dubruel P.

• Polymer Chemistry and Biomaterials (PBM) Group, Ghent University, Krijgslaan 281 S4, 9000 Gent, Belgium

autor

Jansen J. A.

• Department of Biomaterials, Radboud University Medical Center Nijmegen, The Netherlands

autor

Leeuwenburgh S. C. G.

• Department of Biomaterials, Radboud University Medical Center Nijmegen, The Netherlands

Bibliografia

• [1] Chenite, A., et al. (2000). Biomaterials, 21(21), 2155-2161.
• [2] Ruhe, P. Q., et al. (2005). J Control Release, 106(1-2), 162-171.
• [3] Douglas, T. E. L., et al. (2012). Macromol Biosci, Epub ahead of print.

Uwagi

EN

The authors thank Agentschap NL, project no. SHM08717: Research Foundation Flanders (FWO) and Marcin Sobol and Artur Bartkowiak, West Pomeranian University of Technology, Szczecin, Poland.