Exploiting Landfill-Derived Rhodotorula mucilaginosa for Simultaneous Biofuel Synthesis and Leachate Remediation

Wusnah, Wusnah; Supardan, M. Dani; Haryani, Sri; Umi, Fathanah; Yunardi, Yunardi

doi:10.12912/27197050/189243

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Tytuł artykułu

Exploiting Landfill-Derived Rhodotorula mucilaginosa for Simultaneous Biofuel Synthesis and Leachate Remediation

Autorzy

Wusnah Wusnah , Supardan M. Dani , Haryani Sri , Umi Fathanah , Yunardi Yunardi

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DOI

10.12912/27197050/189243

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Abstrakty

Landfill leachate, a complex mixture resulting from decomposing waste, contains suspended and dissolved organic and inorganic compounds. This nutrient-rich environment facilitates the growth of diverse microbial communities that can utilize these compounds for sustenance. Rhodotorula mucilaginosa is a yeast with great potential in the field of biotechnology due to its ability to utilize diverse substrates and its strong resistance to environmental stress. This study was aimed at investigating the potential of R. mucilaginosa, a yeast strain isolated from landfill environments, for biofuel production and simultaneous pollutant reduction in leachate. Batch cultivations were conducted using leachate as the sole growth medium. Cultivation was conducted for 2, 4, 6, and 8 days to analyse the lipids from R. mucilaginosa biomass and the degradation of pollutants in the resulting leachate. Additionally, the fuel properties were determined to assess the quality of the biodiesel produced from R. mucilaginosa lipids. The obtained quality was compared with the American Society for Testing and Materials (ASTM D6751), the Indonesian National Standard (SNI 8968:2021), and the fatty acid methyl ester (FAME) derived from palm oil. Results demonstrated significant lipid accumulation by R. mucilaginosa, reaching 19% (w/w) after 144 hours (6 days) of cultivation. Gas chromatography-mass spectrometry (GC-MS) analysis revealed a FAME profile dominated by C16 and C18 fatty acids, suitable for biodiesel production. Concurrently, substantial reductions in leachate pollutant levels were observed, with decreases of 40.43% for chemical oxygen demand (COD), 86% for phosphate, 90% for ammonia, 53% for nitrate, and 64% for nitrite. These findings highlight the potential of R. mucilaginosa, isolated from landfill leachate, as a promising bioremediation agent for wastewater treatment and a sustainable source of lipids for renewable energy production.

Słowa kluczowe

Rhodotorula mucilaginosa lipid leachate biofuel pollutant

Wydawca

Lubelski Oddział Polskiego Towarzystwo Inżynierii Ekologicznej
Lublin University of Technology
WNGB Wydawnictwo Naukowe

Czasopismo

Ecological Engineering & Environmental Technology

Rocznik

2024

Tom

Vol. 25, iss. 8

Strony

96--108

Opis fizyczny

Bibliogr. 49 poz., rys., tab.

Twórcy

autor

Wusnah Wusnah

wusnah@unimal.ac.id

Postgraduate School of Engineering, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia
Department of Chemical Engineering, Faculty of Engineering, Universitas Malikussaleh, Lhokseumawe, North Aceh, Indonesia

https://orcid.org/0000-0002-2983-993X

autor

Supardan M. Dani

m.dani.supardan@usk.ac.id

Department of Chemical Engineering, Faculty of Engineering, Universitas Syiah Kuala, Darussalam, Banda Aceh 23111, Indonesia

https://orcid.org/0000-0002-1665-9024

autor

Haryani Sri

sri.haryani@usk.ac.id

Department of Agricultural Products Technology, Faculty of Agriculture, Universitas Syiah Kuala, Darussalam, Banda Aceh, Indonesia

https://orcid.org/0000-0002-8073-4068

autor

Umi Fathanah

umifathanah@usk.ac.id

Department of Chemical Engineering, Faculty of Engineering, Universitas Syiah Kuala, Darussalam, Banda Aceh 23111, Indonesia

https://orcid.org/0009-0006-1019-324X

autor

Yunardi Yunardi

yunardi@usk.ac.id

Department of Chemical Engineering, Faculty of Engineering, Universitas Syiah Kuala, Darussalam, Banda Aceh 23111, Indonesia

https://orcid.org/0000-0003-4362-0392

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