The evaluation of resorbable haemostatic wound dressings in contact with blood in vitro

• Autorzy: Szymonowicz M. , Kucharska M. , Wiśniewska-Wrona M. , Dobrzyński M. , Kołodziejczyk K. , Rybak Z.

Identyfikatory

DOI

10.5277/ABB-00523-2015-04

• Języki publikacji: EN

Abstrakty

EN

Purpose: For many years research has been conducted on the development of resorbable, polymer, haemostatic materials designed to provide first aid and preliminary protection of injuries. The biological properties in vitro of a dressing in powder form called Hemoguard are expected to provide the ability to instantaneously stem bleeding with safe conditions of use. The aim of the study was to evaluate the haemostatic properties of a model of dressing based on micro- and nanofibrids of the chitosan, sodium/calcium alginate and/or carboxymethylcellulose complex. Dressings were prepared by spray-drying and freeze-drying. Methods: Human whole blood was subjected to timed contact with the haemostatic dressing model. Haemolytic action was determined by assaying the degree of haemolysis and evaluating blood cell morphology. Haemostatic action was determined on the basis of selected parameters of plasmatic clotting systems. Results: Dressings prepared by freeze-drying activated the coagulation system. The haemolytic index, plasma haemoglobin concentration values and blood cell morphological shapes were normal. Dressings prepared by spray-drying significantly activated coagulation. Activation of the coagulation process was evidenced by shorter clotting time of the plasma coagulation system and a longer process of clot formation. The dressing was associated with an increased haemolytic index and higher plasma haemoglobin concentration. The morphological shape of blood cells changed. Conclusions: The model of multi-resorbable wound dressings has haemostatic properties. The materials activate the clotting process more quickly than a single-dressing model. Increased activity was found for dressings prepared by spray-drying.

Słowa kluczowe

EN

haemostatic; dressing; chitosan-alginate; nanofibrids; microfibrids; haemolysis; plasmatic clotting system

PL

opatrunek; chitozan; alginian; mikrofibrydy; nanofibrydy; hemoliza

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2017
• Tom: Vol. 19, no. 1
• Strony: 151--165
• Opis fizyczny: Bibliogr. 30 poz., rys., tab., wykr.

Twórcy

autor

Szymonowicz M.

• Department of Experimental Surgery and Biomaterials Research, Medical University, Wrocław, Poland

autor

Kucharska M.

• Institute of Biopolymers and Chemical Fibres, Łódź, Poland

autor

Wiśniewska-Wrona M.

• Institute of Biopolymers and Chemical Fibres, Łódź, Poland

autor

Dobrzyński M.

• Department of Conservative Dentistry and Pedodontics, Medical University, Wrocław, Poland

autor

Kołodziejczyk K.

• Department of Experimental Surgery and Biomaterials Research, Medical University, Wrocław, Poland

autor

Rybak Z.

• Department of Experimental Surgery and Biomaterials Research, Medical University, Wrocław, Poland

Bibliografia

• [1] ASTM, F 756-08:2008. Standard practice for assessment of hemolytic properties of materials.
• [2] CHENG Y.H., YANG S.H., LIU C.C., GEFEN A., LIN F.H., Thermosensitive hydrogel made of ferulic acid-gelatin and chitosan glycerophosphate, Carbohydr. Polym., 2013, 92, 1512–1519.
• [3] DEVLIN J.J., KIRCHER S., KOZEN B.G., LITTLEJOHN L.F., JOHNSON A.S., Comparison of chitoflex®, celox™, and quikclot® in control of hemorrhage, J. Emerg. Med., 2011, 41(3), 237–245.
• [4] ENGLEHART M.S., CHO S.D., TIEU B.H., MORRIS M.S., UNDERWOOD S.J., KARAHAN A., MULLER P.J., DIFFERDING J.A., FARRELL D.H., SCHREIBER M.A., A novel highly porous silica and chitosan-based hemostatic dressing is superior to HemCon and gauze sponges, J. Trauma, 2008, 65, 884–890.
• [5] GEGEL B., BURGERT J., COOLEY B., MACGREGOR J., MYERS J., CALDER S., JOHNSON D., The effects of BleedArrest, Celox, and TraumaDex on hemorrhage control in a porcine model, J. Surg. Res., 2010, 164(1), 125–129.
• [6] GUSTAFSON S.B., FULKERSON P., BILDFELL R., AGUILERA L., HAZZARD T.M., Chitosan dressing provides hemostasis in swine femoral arterial injury model, Prehosp. Emerg. Care, 2007, 11, 172–178.
• [7] ISO 10993-4: 2009. Biological evaluation of medical devices. Part 4: Selection of tests for interactions with blood.
• [8] KHEIRABADI B.S., Evaluation of topical hemostatic agents for combat wound treatment, US Army Med. Dep. J., 2011, 2(1), 25–37.
• [9] KOZEN B.G., KIRCHER S.J., HENAO J., An alternative hemostatic dressing: comparison of Celox, HemCon, and QuikClot, Acad. Emerg. Med., 2008, 15, 74–81.
• [10] KRUCIŃSKA J., KOMISARCZYK A., PALUCH D., SZYMONOWICZ M., ŻYWICKA B., PIELKA S., The impact of the dibutyrylchitin molar mass on the bioactive properties of dressings used to treat soft tissue wounds, J. Biomed. Mater Res. Part B, 2012, 1, 11–22.
• [11] KUCHARSKA M., STRUSZCZYK M.H., CICHECKA M., BRZOZA- -MALCZEWSKA K., WIŚNIEWSKA-WRONA M., Prototypes of primary wound dressing of fibrous and quasi-fibrous structure in terms of safety of their usage, Fibres Text. East. Eur., 2012, 20(6), 142–148.
• [12] KUCHARSKA M., CIECHAŃSKA D., NIEKRASZEWICZ A., WIŚNIEWSKA-WRONA M., KARDAS I., Potential use of chitosanbased materials in medicine, PCACD, 2010, 15, 169–176.
• [13] KUCHARSKA M., NIEKRASZEWICZ A., CIECHAŃSKI D., WITCZAK E., STRUSZCZYK M.H., GULBA-DIAZ A., SUJKA W., TROMBOGUARD® – nowoczesny opatrunek pierwszej pomocy (TROMBOGUARD® – modern first aid dressing), Mil. Phar. Med., 2010, 15(2), 22–28.
• [14] KUCHARSKA M., WIŚNIEWSKA-WRONA M., BRZOZA- -MALCZEWSKA K., STRUSZCZYK M.H., CICHECKA M., WILBIK-HAŁGAS B., RYBAK Z., SZYMONOWICZ M., PALUCH D., GUZIŃSKA K., KAŹMIERCZAK D., Haemostatic, resorbable dressing of natural polymers-Hemoguard, PCACD, 2015, 20, 130–141.
• [15] LITTLEJOHN L.F., DEVLIN J.J., KIRCHER S.S., Comparison of Celox-A, ChitoFlex, WoundStat, and Combat Gauze hemostatic agents versus standard gauze dressing in control of hemorrhage in a swine model of penetrating trauma, Acad. Emerg. Med., 2011, 18(4), 340–350.
• [16] MUZZARELLI R.A., Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone, Carbohydr. Polym., 2009, 76, 67–182.
• [17] PALMER B.L., GANTT D.S., LAWRENCE M.E., RAJAB M.H., DEHMER G.J., Effectiveness and safety of manual hemostasis facilitated by the Syvek-Patch with one hour of bedrest after coronary angiography using six-French catheters, Am. J. Cardiol., 2004, 93, 96–97.
• [18] PIGHINELLI L., KUCHARSKA M., Chitosan-hydroxyapatite composites, Carbohydr. Polym., 2013, 93, 256–262.
• [19] Polish patent appl. P.390253 Opatrunek hemostatyczny warstwowy i sposób wytwarzania opatrunku hemostatycznego warstwowego (Multilayer haemostatic dressing and method to produce the dressing).
• [20] RAN Y., HADAD E., DAHER S., QuikClot Combat Gauze Use for Hemorrhage Control in Military Trauma: January 2009 Israel defense force experience in the Gaza strip – a preliminary report of 14 Cases, Prehop. Disaster Med., 2010, 25(6), 584–588.
• [21] SZELEST-LEWANDOWSKA A., MASIULANIS M., SZYMONOWICZ M., PIELKA S., PALUCH D., Modified Poly(carbonateurethane). Synthesis, properties and biological investigation in vitro, J. Biomed. Mat. Res. Part A, 2007, 82(12), 509–551.
• [22] SZYMONOWICZ M., FRĄCZEK-SZCZYPTA A., RYBAK Z., BŁAŻEWICZ S., Comparative assessment of the effect of carbon-based material surfaces on blood clotting activation and haemolysis, Diam. Rel. Mate.r, 2013, 40, 89–95.
• [23] SZYMONOWICZ M., KRATOCHWIL J., RUTOWSKI R., STANISZEWSKA-KUŚ J., PALUCH D., Evaluation of the influence of topical haemostatics materials on coagulation and fibrinolysis parameters, Eng. Biomat., 1999, 7(8), 45–52.
• [24] SZYMONOWICZ M., PALUCH D., SOLSKI L., PIELKA S., BŁASIŃSKA A., KRUCIŃSKA J., Evaluation of the influence of dibutyrylchitin materials for activation of blood coagulation System, Eng. Biomat., 2004, 7, 123–126.
• [25] SZYMONOWICZ M., PIELKA S., OWCZAREK A, HAZNAR D., PLUTA D., Study on influence of gelatin-alginate matrixes on the coagulation system and morphotic blood elements, Macromol. Symp., 2007, 253, 71–76.
• [26] SZYMONOWICZ M., RYBAK Z., FRĄCZEK-SZCZYPTA A., PALUCH D., RUSAK A., NOWICKA K., BŁAŻEWICZ M., Haemocompatibility and cytotoxic studies of non-metallic composite materials modified with magnetic nano and microparticles, Acta Bioeng. Biomech., 2015, 17(3), 49–58.
• [27] SZYMONOWICZ M., RYBAK Z., WITKIEWICZ W., PEZOWICZ C., FILIPIAK J., In vitro hemocompatibility studies of (poly(L-lactide) and poly(L-lactide-co-glycolide) as materials for bioresorbable stents manufacture, Acta Bioeng. Biomech., 2014, 16(4), 131–139.
• [28] WARD K.R., TIBA M.H., HOLBERT W.H., Comparison of a new hemostatic agent to current combat hemostatic agents in a swine model of lethal arterial hemorrhage, J. Trauma., 2007, 63, 276–284.
• [29] WIŚNIEWSKA-WRONA M., KUCHARSKA M., KARDAS I., BODEK A., BODEK K.H., Polymer biocomposites used in bedsores treatment, PCACD, 2011, 16, 111–120.
• [30] ZHANG J., XIA W., LIU P., CHENG Q., TAHI T., GU W., LI B., Chitosan modification and pharmaceutical/biomedical applications, Mar. Drugs, 2010, 8(7), 1962–1987.

Uwagi

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).

This research was carried out under a project contained in The Programme of Applied Research No PBS1/B7/5/2012 sponsored by the National Centre of Research and Development.