Statistical methods of data analysis in obtaining thyme oil-loaded nanoemulsions as a potential skin antiseptic

• Autorzy: Miastkowska Małgorzata , Łętocha Anna , Michalczyk Alicja

Identyfikatory

DOI

10.24425/cpe.2023.147395

• Konferencja: 24th Polish Conference of Chemical and Process Engineering, 13-16 June 2023, Szczecin, Poland. Guest editor: Prof. Rafał Rakoczy
• Języki publikacji: EN

Abstrakty

EN

The two aims of this study were to obtain stable thyme-oil loaded nanoemulsions using the statistical design of experiment method (DOE) and to confirm their antimicrobial and disinfecting properties. Thyme oil was used as the oil phase, ECO Tween R©80 acted as an emulsifier, and the rest of the formulation was deionized water. Ultrasonication was chosen as the method of obtaining the nanoemulsions. It was checked whether the input parameters (oil concentration, emulsifier concentration, amplitude, and sonication time) had a significant impact on the output parameters (nanoemulsion particle size, polydispersity index, viscosity, and stability over time). For the formulations selected on the basis of the statistical data analysis, the values of minimum inhibitory concentrations (MIC) and minimum biocidal concentrations (MBC/MFC) were determined in relation to 10 bacterial strains and 10 strains of fungi (filamentous fungi, yeast-like fungi). The results obtained from the statistical analysis showed that the optimal concentration of the thyme oil in nanoemulsion should amount up to 2%. Biological studies proved that the obtained formulation had stronger antibacterial and antifungal activity compared to pure oil. Moreover, it was shown that the nanoemulsion caused a reduction of > 5 log of bacterial strains (S. aureus, P. aeruginosa) and > 4 log of fungal strains (C. albicans) after 30 minutes, a level required for disinfectants.

Słowa kluczowe

EN

nanoemulsions; thyme oil; antimicrobial activity; disinfectant; design of experiment

PL

nanoemulsje; olejek tymiankowy; aktywność przeciwdrobnoustrojowa; środek dezynfekujący; projekt eksperymentu

• Wydawca: Komitet Inżynierii Chemicznej i Procesowej Polskiej Akademii Nauk
• Czasopismo: Chemical and Process Engineering : New Frontiers
• Rocznik: 2023
• Tom: Vol. 44, nr 4
• Strony: art. no. e36
• Opis fizyczny: Bibliogr. 48 poz., rys., tab.

Twórcy

autor

Miastkowska Małgorzata

• Cracow University of Technology, Department of Chemical Engineering and Technology, Warszawska 24, 31-155 Krakow, Poland

• https://orcid.org/0000-0002-4590-9184

autor

Łętocha Anna

• Cracow University of Technology, Department of Chemical Engineering and Technology, Warszawska 24, 31-155 Krakow, Poland

autor

Michalczyk Alicja

• Lukasiewicz – Research Network-Institute of Industrial Organic Chemistry, Annopol 6, 03-236 Warsaw, Poland

• https://orcid.org/0000-0002-9766-3227

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr POPUL/SP/0154/2024/02 w ramach programu "Społeczna odpowiedzialność nauki II" - moduł: Popularyzacja nauki (2025)