Development and validation of stability-indicating RP-HPLC method for the simultaneous determination of tizanidine HCL and meloxicam in rabbit's plasma

• Autorzy: Zaman Muhammad , Hanif Muhammad , Khan Najm Ul Hassan , Mahmood Asif , Qaisar Muhammad Naeem , Ali Huma

Identyfikatory

DOI

10.1556/1326.2018.00408

• Języki publikacji: EN

Abstrakty

EN

High-performance liquid chromatography (HPLC) is a widely used technique for the simultaneous detection and quantification of different drugs. The purpose of the current study was to develop a simple and cost-effective reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous determination of tizanidine (TZN) HCl and meloxicam (MLX) in rabbit's plasma. Assay of TZN and MLX was performed after extraction of drug from plasma by liquid–liquid extraction technique using methanol and diethyl ether as protein precipitants. Isocratic elution was performed in a Kromasil® C18 column (dimension, 250 × 4.60 mm; particle size, 5 μm) with mobile phase consisting of methanol–water (8:2). Orthophosphoric acid was used to adjust the pH of the mobile phase 3.0, and detection was done at 228 nm. Flow rate was 0.8 mL/min with ambient temperature and average operating pressure of 1400 psig. Retention time of TZN was 2.612 min and that of MLX was 6.960 min with a resolution of 3.18. Both drugs showed satisfactory linearity in the range of 10 to 50 ng/mL with correlation coefficients (R²) of 0.9989 and 0.9972 for TZN and MLX, respectively. The developed method was validated successfully for linearity, system suitability, intra-day and inter-day accuracy, and precision, robustness, and specificity following International Conference on Harmonization (ICH) guidelines. Conclusively, a precise, stable, reproducible, economical, and suitable method for estimation of pharmacokinetic evaluation was developed and validated.

Słowa kluczowe

EN

Tizanidine HCl; meloxicam; rabbit plasma; method development; validation; simultaneous determination; RP-HPLC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2019
• Tom: Vol. 31, no. 3
• Strony: 173--178
• Opis fizyczny: Bibliogr. 32 poz., rys., tab.

Twórcy

autor

Zaman Muhammad

• Department of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan
• Department of Pharmaceutics, Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan

autor

Hanif Muhammad

• Department of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan

autor

Khan Najm Ul Hassan

• Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan

autor

Mahmood Asif

• Department of Pharmaceutics, Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan

autor

Qaisar Muhammad Naeem

• College of Pharmacy, University of Sargodha, Sargodha, Pakistan

autor

Ali Huma

• Department of Pharmaceutical Sciences, Jinnah Sindh Medical College University, Karachi, Pakistan

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).