PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
Tytuł artykułu

Quantitative estimation of mimosine in anti-psoriatic ayurvedic formulation containing whole plant extract of Mimosa pudica Linn. by validated isocratic RP-HPLC method

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In this chapter, an isocratic reverse phase HPLC determination of mimosine was developed and validated in an anti-psoriatic topically applied formulation “Lajjalu”. The chromatography was performed on a C18 column with water-orthophosphoric acid (98.8:0.2, υ/υ) as a mobile phase with a pH of 3.0 at a flow rate of 1.0 mL min-1. Detection was performed at 284 nm, and a sharp peak was obtained for mimosine at a retention time of 2.62 ± 0.01 min. Linear regression analysis data for the calibration plot showed a good linear relationship between response curve and concentration in the range of 0.050–5000 ng mL-1 and the regression coefficient was 0.9998 with the linear regression equation y = 4766.8x−17726. The detection (LOD) and quantification (LOQ) limits were 10.3 and 35.6 ng mL-1, respectively. The wide linearity range, sensitivity, accuracy, short retention time, and simple mobile phase imply the method is suitable for routine quantification of mimosine with high precision and accuracy.
Rocznik
Strony
531--538
Opis fizyczny
Bibliogr. 18 poz., rys., tab.
Twórcy
autor
  • Hamdard University (Jamia Hamdard) Faculty of Pharmacy, Department of Pharmaceutics Hamdard Nagar, New Delhi 110 062 India
autor
  • Hamdard University (Jamia Hamdard) Faculty of Pharmacy, Department of Pharmacognosy and Phytochemistry Hamdard Nagar, New Delhi 110 062 India
autor
  • Hamdard University (Jamia Hamdard) Faculty of Pharmacy, Department of Pharmacognosy and Phytochemistry Hamdard Nagar, New Delhi 110 062 India
autor
  • Hamdard University (Jamia Hamdard) Faculty of Pharmacy, Department of Pharmacognosy and Phytochemistry Hamdard Nagar, New Delhi 110 062 India
autor
  • Hamdard University (Jamia Hamdard) Faculty of Pharmacy, Department of Pharmaceutics Hamdard Nagar, New Delhi 110 062 India
autor
  • Hamdard University (Jamia Hamdard) Faculty of Pharmacy, Department of Pharmaceutics Hamdard Nagar, New Delhi 110 062 India
Bibliografia
  • [1] K.B. Gordon, R.R. Blum, K.A. Papp, R. Matheson, C. Bolduc, T. Hamilton, M. Heffernan, M.A. Menter, M.M. Okun, J. Zhong, M. Kaul, and A. Camez, Psoriasis Forum 13, 4–11 (2007)
  • [2] A.J. Hueber and I.B. McInnes, Immunol. Lett., 114, 59–65 (2007)
  • [3] Nimbatiktam for the Treatment of Psoriasis, http://www.nrdcindia.com/pages/nimbatiktam.htm [last accessed on 18 January 2010]
  • [4] J.A. Duke and M.J. Bogenschutz-Godwin, Handbook of Medicinal Herbs, 2nd edn, CRC Press, 2002, pp. 663–664.
  • [5] R. Puchala, S.G. Pierzynowski, T. Sahlu, and S.P. Hart, Br. J. Nutr., 15, 69–79 (1996)
  • [6] M. Soedarjo, T.K. Hemscheidt, and D. Borthakur, Appl. Environ. Microbiol., 60, 4268–4272 (1994)
  • [7] S. Vaithiyanathan, Q. Sheikh, and R. Kumar, Asian Austral. J. Anim. Sci., 18, 332–339 (2005)
  • [8] P.R. Abel and G. Ruth, World Patent WO2007129133 (2007)
  • [9] G.Z. Moises, M.L. Ruben, and P.D. Antonio, European Patent 20070704740 (2008)
  • [10] L.S. Nair, S.N. Menon, S. Shailajan, M.M. Baing, and R.T. Sane, J. Planar Chromatogr., 20, 49–51 (2007)
  • [11] K. Lalitha and S.R. Kulothungan, Talanta, 63, 635–640 (2004)
  • [12] K. Lalitha and S.R. Kulothungan, Amino Acids, 31, 279–287 (2006)
  • [13] R.G. Megarrity, J. Sci. Food Agric., 29, 182–186 (2006)
  • [14] R. Puchala, J.J. Davis, and T. Sahlu, J. Chromatogr., B, 685, 375–378 (1996)
  • [15] CH, Q2 (R1), Validation of Analytical Procedures: Text and Methodology. International Conference on Harmonization, Geneva, http://www.ich.org/LOB/media/MEDIA417.pdf (2005)
  • [16] S. Baboota, S. Faiyaz, A. Ahuja, J. Ali, and S. Ahmad, Acta. Chromatogr., 18, 116–129 (2007)
  • [17] M.J. Ansari, S. Ahmad, K. Kohli, J. Ali, and R.K. Khar, J. Pharm. Biomed. Anal., 39, 132–136 (2005)
  • [18] S. Ahmad, R. Ahmad, R. Parveen, and M. Aquil, Chromatographia, 67, 41–47 (2008)
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-8d04b8e6-bb3e-44f5-b84a-cbe74cffec49
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.