Quantitative estimation of mimosine in anti-psoriatic ayurvedic formulation containing whole plant extract of Mimosa pudica Linn. by validated isocratic RP-HPLC method

• Autorzy: Musthaba S , Athar M. T , Kamal Y. T , Ahmad S. , Ali J. , Baboota S.
• Języki publikacji: EN

Abstrakty

EN

In this chapter, an isocratic reverse phase HPLC determination of mimosine was developed and validated in an anti-psoriatic topically applied formulation “Lajjalu”. The chromatography was performed on a C18 column with water-orthophosphoric acid (98.8:0.2, υ/υ) as a mobile phase with a pH of 3.0 at a flow rate of 1.0 mL min^-1. Detection was performed at 284 nm, and a sharp peak was obtained for mimosine at a retention time of 2.62 ± 0.01 min. Linear regression analysis data for the calibration plot showed a good linear relationship between response curve and concentration in the range of 0.050–5000 ng mL^-1 and the regression coefficient was 0.9998 with the linear regression equation y = 4766.8x−17726. The detection (LOD) and quantification (LOQ) limits were 10.3 and 35.6 ng mL^-1, respectively. The wide linearity range, sensitivity, accuracy, short retention time, and simple mobile phase imply the method is suitable for routine quantification of mimosine with high precision and accuracy.

Słowa kluczowe

EN

mimosine; isocratic RP-HPLC method; anti-psoriatic; Mimosa pudica; Lajjalu; coconut oil

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2011
• Tom: Vol. 23, no. 3
• Strony: 531--538
• Opis fizyczny: Bibliogr. 18 poz., rys., tab.

Twórcy

autor

Musthaba S

• Hamdard University (Jamia Hamdard) Faculty of Pharmacy, Department of Pharmaceutics Hamdard Nagar, New Delhi 110 062 India

autor

Athar M. T

• Hamdard University (Jamia Hamdard) Faculty of Pharmacy, Department of Pharmacognosy and Phytochemistry Hamdard Nagar, New Delhi 110 062 India

autor

Kamal Y. T

• Hamdard University (Jamia Hamdard) Faculty of Pharmacy, Department of Pharmacognosy and Phytochemistry Hamdard Nagar, New Delhi 110 062 India

autor

Ahmad S.

• Hamdard University (Jamia Hamdard) Faculty of Pharmacy, Department of Pharmacognosy and Phytochemistry Hamdard Nagar, New Delhi 110 062 India

autor

Ali J.

• Hamdard University (Jamia Hamdard) Faculty of Pharmacy, Department of Pharmaceutics Hamdard Nagar, New Delhi 110 062 India

autor

Baboota S.

• Hamdard University (Jamia Hamdard) Faculty of Pharmacy, Department of Pharmaceutics Hamdard Nagar, New Delhi 110 062 India

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