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Języki publikacji
Abstrakty
Alginate – chitosan – alginate multilayer hydrogel encapsulation systems were investigated for encapsulation of chondrocytes. Hydrogel is crosslinked due to ionic interaction between cationic chitosan and anionic alginate, and additionally by calcium ions. Two types of chitosan with molecular weight were investigated. Cells were encapsulated in two shape microcapsules, microbeads with diameter size 300 – 400 and 500 - 600 [mi]m and fibres with diameter 500 - 600 [mi]m. The work provides a detailed examination of the impact of the microencapsulation process on the growth of cells. The viability of chondrocytes can be influenced by the size of produced microcapsules, while the shape of microcapsules has no important significance on cell viability. The applied encapsulation methods do not contain harmful stages and create conducive conditions for cell growth. A possible application area of the developed system is dressing and regeneration of damaged joint cartilage.
Czasopismo
Rocznik
Tom
Strony
529--538
Opis fizyczny
Bibliogr. 17 poz., rys., tab., fot.
Twórcy
autor
- Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warszawa, Poland
autor
- Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warszawa, Poland
Bibliografia
- 1. Chandra R., Rustgi R., 1998. Biodegradable polymers. Prog. Polym. Sci., 23, 1273–1335. DOI: 10.1016/S00796700(97)00039-7.
- 2. Chia S.H., Homicz M.R., Schumacher B.L., Thonar E.J.-M.A., Masuda K., Sah R.L., Watson D., 2005. Characterization of human nasal septal Chondrocytes cultured in alginate. J. Am. College Surg.,, 200, 691–704. DOI: 10.1016/j.jamcollsurg.2005.01.006.
- 3. Ciach T., 2007, Application of electro hydro dynamic atomization in drug delivery, J. Drug Deliv. Sci. Technol., 17, 367-375.
- 4. Constantinidis J., Rask J., Long R.C. Jr., Sambanis A., 1999. Effect of alginate composition on the metabolic, secretory, and growth characteristics of entrapped ßTC3 muse insulinoma cells. Biomaterials, 20, 2019–2027. DOI: 10.1016/S0142-9612(99)00104-0.
- 5. De S., Robinson D., 2003. Polymer relationships during preparation of chitosan – alginate and poly-l-lysine – alginate nanospheres. J. Control. Release, 89, 102–112 DOI: 10.1016/S0168-3659(03)00098-1
- 6. Domm C., Schünke M., Christesen K., Kurz B., 2002. Redifferentiation of dedifferentiated bovine articular chondrocytes in alginate culture under low oxygen tension. Osteoarthr. Cartil., 10, 13–22. DOI: 10.1053/joca.2001.0477.
- 7. Gaserod O., Sannes A., Skjak-Brak G., 1999. Microencapsulation of alginate chitosan. II. A study of capsule stability and permeability. Biomaterials, 20, 773-783. DOI: 10.1016/S0142-9612(98)00230-0.
- 8. Gaserod O., Smidsrod O., Skjak-Brak G., 1999. Microcapsules of alginate chitosan - I: A quantative study of interaction between alginate and chitosan. Biomaterials, 19, 1815–1825. DOI: 10.1016/S0142-9612(98)00073-8.
- 9. Gombotz W.R, Wee S.F, 1999. Protein release from alginate matrices. Adv. Drug Deliv. Rev., 31, 267–285. DOI: 10.1016/S0169-409X(97)00124-5.
- 10. Haque T., Chen H., Ouyang W., Martoni Ch., Lawuyi B., Urbańska A.M., Prakash S., 2005. In vitro study of alginate – chitosan microcapsules: An alternative to liver cell transplants for the treatment of liver failure. Biotechnol. Lett., 27, 317-322. DOI: 10.1007/s10529-005-0687-3.
- 11. Lim F., Sun A.M., 1980. Microencapsulated islets as bioartificial endocrine pancreas. Sci., 210, 908–910. DOI: 10.1126/science.6776628.
- 12. Moresi M., Bruno M., 2007. Characterisation of alginate gel using quasi-stati and dynamic methods. J. Food Eng., 82, 298–309. DOI: 10.1016/j.jfoodeng.2007.02.040.
- 13. Nafea E.H, Marson A., Poole-Warren L.A, Martens P.J., 2011. Immunoisolating semi-permeable membranes for cell encapsulation: Focus on hydrogels. J. Control. Release, 154, 110–122. DOI: 10.1016/j.jconrel.2011.04.022.
- 14. Puppi D., Chiellini F., Piras A.M., Chiellini E., 2010. Polymeric materials for bone and cartilage repair. Prog. Polym. Sci., 35, 403-440. DOI: 10.1016/j.progpolymsci.2010.01.006.
- 15. Sittinger M., Braunling J., Kastenbauer E., Hammmer C., Gburmester ,Bujie J., 1997. Analysis of the proliferative potential of human nasal chondrocytes for the engineering of cartilage transplants. Laryngo–Rhino-Otol., 76, 96 –100. DOI: 10.1055/s-2007-997394.
- 16. Stabler C., Wilks K., Sambanis A., Constantinidis I., 2001. The effects of alginate composition on encapsulated SSTC3 cells. Biomaterials, 22, 1301–1310. DOI: 10.1016/S0142-9612(00)00282-9.
- 17. Wang L., Shelton R.M., Cooper P.R., Lawson M., Triffitt J.T., Barralet J.E., 2003. Evaluation of sodium alginate for bone narrow cell tissue engineering. Biomaterials, 24, 3475–3481. DOI: 10.1016/S0142-9612(03)00167-4.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-8cf94fd4-a475-4b90-a436-74697c2732c0