Bacterial selection of the Pseudomonas genus with the capacity to treat water and contaminated soils

• Autorzy: Escudero-López Henry Joel , Serrano-Carrillo Karla Anabel , Jácome-Pilco Carlos Rodrigo , Sanaguano-Salguero Herminia del Rosario , Bayas-Morejón Isidro Favian

Identyfikatory

DOI

10.24425/jwld.2022.140803

• Języki publikacji: EN

Abstrakty

EN

In the present work, bacteria of the Pseudomonas genus native to the Ecuadorian Amazon with the capacity to treat contaminated water and soils were selected. For this purpose, 20 soil samples from Amazon region with evidence of contamination were analysed. For identification, each sample was assigned a code according to the sampling area: Joya de los Sachas (S), Minga (M) and Siete de Julio-Shushufindi (SH). The cultures were performed in the combination of Bushnell Hass (BH) + Luria Bertani (LB) and Müeller-Hinton (MH) + Brucella agar (BA) media, all with the addition of diesel to verify their efficacy in the growth of bacteria capable of surviving in contaminated media. The combination with ideal results was that of BH + LB, by means of Gram-staining it was determined that 19 of the samples had interest microorganisms. To characterize the isolates at the species level, biochemical tests of: catalase, citrate, glucose, hemolytic activity and urease were applied, which allowed to confirm the existence of the Pseudomonas of interest. The results indicated that P. stutzeri (in samples S1 and M1), P. aeruginosa (in SH2 and SH5) and P. putida (in S7, S8, S10 and SH4) obtaining a total of 8 isolates (40%) of interest from the initial 19. With the results obtained from this work, an optimal culture method was standardized for the selection of bacteria with potential for treating contaminated soils and water.

Słowa kluczowe

EN

bioremediation; characterisation; contaminated water; contaminated soils; Ecuadorian Amazon; Pseudomonas genus

• Wydawca: Wydawnictwo ITP
• Czasopismo: Journal of Water and Land Development
• Rocznik: 2022
• Tom: no. 53
• Strony: 238--241
• Opis fizyczny: Bibliogr. 22 poz., tab.

Twórcy

autor

Escudero-López Henry Joel

• State University of Bolívar, Faculty of Agricultural Sciences, Agroindustry Career, Biotechnological Research and Development Center, Km 3 1/2 sector Alpachaca, CP: 020150, Guaranda, Ecuador

• https://orcid.org/0000-0001-9452-8938

autor

Serrano-Carrillo Karla Anabel

• San Pedro Educational Unit, Department of Education, Guaranda, Ecuador

• https://orcid.org/0000-0002-5783-8184

autor

Jácome-Pilco Carlos Rodrigo

• State University of Bolívar, Faculty of Agricultural Sciences, Agroindustry Career, Biotechnological Research and Development Center, Km 3 1/2 sector Alpachaca, CP: 020150, Guaranda, Ecuador

• https://orcid.org/0000-0002-9713-0228

autor

Sanaguano-Salguero Herminia del Rosario

• State University of Bolívar, Faculty of Agricultural Sciences, Agroindustry Career, Biotechnological Research and Development Center, Km 3 1/2 sector Alpachaca, CP: 020150, Guaranda, Ecuador

• https://orcid.org/0000-0003-0010-6019

autor

Bayas-Morejón Isidro Favian

• State University of Bolívar, Faculty of Agricultural Sciences, Agroindustry Career, Biotechnological Research and Development Center, Km 3 1/2 sector Alpachaca, CP: 020150, Guaranda, Ecuador

• https://orcid.org/0000-0003-2920-7155

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).