Simultaneous RP-HPLC quantification of vasicine and vasicinone in Adhatoda vasica and its formulation

• Autorzy: Suthar A , Hamarapurkar P , Patil P , Phale M , Katkar K , Mundada G , Chauhan V. S
• Języki publikacji: EN

Abstrakty

EN

A simple, specific, and precise RP-HPLC method with photodiode-array detection has been developed for simultaneous analysis of the quinazoline alkaloids vasicine and vasicinone, pharmacologically important constituents of Adhatoda vasica . The compounds were separated by use of 0.1% trifluoroacetic acid in water:acetonitrile 90:10 as isocratic mobile phase at a flow rate of 1.0 mL min ^-1 Under these conditions, plots of integrated peak area against concentration were linear over the ranges 0.05–10.0 μg mL ^-1 and 0.5–100.0 μg mL ^-1 ( r 2 = 0.999 and 0.998) for vasicine and vasicinone, respectively. Mean recovery was 99.06% for vasicine and 98.69% for vasicinone. The method is therefore suitable for routine analysis of vasicine and vasicinone in Adhatoda vasica plants of different varieties and in herbal medicinal products prepared from the plants.

Słowa kluczowe

EN

Adhatoda vasica; vasicine; vasicinone; RP-HPLC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2010
• Tom: Vol. 22, no. 4
• Strony: 599--608
• Opis fizyczny: Bibliogr. 13 poz., rys., tab.

Twórcy

autor

Suthar A

• Piramal Life Sciences Ltd Herbal Department 1 Nirlon Complex, Off Western Express Highway, Goregaon (East) Mumbai 400 063 Maharashtra India

autor

Hamarapurkar P

• Principal K.M. Kundnani College of Pharmacy Pharmaceutical Analysis Jote Joy Building, Rambhau Salgaoankar Marg Cuffe Parade, Mumbai 400 005 Maharashtra India

autor

Patil P

• Principal K.M. Kundnani College of Pharmacy Pharmaceutical Analysis Jote Joy Building, Rambhau Salgaoankar Marg Cuffe Parade, Mumbai 400 005 Maharashtra India

autor

Phale M

• Principal K.M. Kundnani College of Pharmacy Pharmaceutical Analysis Jote Joy Building, Rambhau Salgaoankar Marg Cuffe Parade, Mumbai 400 005 Maharashtra India

autor

Katkar K

• Piramal Life Sciences Ltd Herbal Department 1 Nirlon Complex, Off Western Express Highway, Goregaon (East) Mumbai 400 063 Maharashtra India

autor

Mundada G

• Piramal Life Sciences Ltd Herbal Department 1 Nirlon Complex, Off Western Express Highway, Goregaon (East) Mumbai 400 063 Maharashtra India

autor

Chauhan V. S

• Piramal Life Sciences Ltd Herbal Department 1 Nirlon Complex, Off Western Express Highway, Goregaon (East) Mumbai 400 063 Maharashtra India

Bibliografia

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