Analiza lipidomiczna w diagnostyce chorób mózgu

• Autorzy: Bogusiewicz Joanna , Burlikowska Katarzyna , Kupcewicz Bogumiła , Łuczykowski Kamil , Jaroch Karol , Goryńska Paulina Zofia , Goryński Krzysztof , Birski Marcin , Furtak Jacek , Paczkowski Dariusz , Harat Marek , Pawliszyn Janusz , Bojko Barbara

Identyfikatory

DOI

10.53584/wiadchem.2025.1.2

Warianty tytułu

EN

Lipidomic analysis in brain tumor diagnosis

• Języki publikacji: PL

Abstrakty

EN

Fast and accurate diagnosis of brain tumors is a critical step in effective patient therapy. However, challenges arise due to the complex anatomy of the brain and the late manifestation of symptoms, making diagnosis often difficult. Therefore, interdisciplinary research at the intersection of analytical chemistry, medical diagnostics, and chemical engineering is essential to develop new diagnostic approaches that facilitate the analysis of neoplastic changes in the brain. One of many promising technologies is solid-phase microextraction (SPME), also known as chemical biopsy. This method allows for the extraction of compounds from biological samples and solid ones such as tumors. However, selecting potential biomarkers is equally important to developing and optimizing appropriate analytical tools. Lipids have recently gained attention for their significant roles in cancer processes. Thus, the applicability of SPME probes in the analysis of brain tumor lipidome was assessed. Chemical biopsy was used in the analysis of brain tumors, revealing that the lipidomic profiles of benign meningiomas significantly differed from those obtained from malignant lesions, such as gliomas. Furthermore, it was proven that sampling using the SPME probe was reliable. Studies focusing on gliomas demonstrated that the lipidome of these brain tumors varied depending on the grade of malignancy and the status of IDH1/2 mutations. Moreover, it was also proven that acylcarnitines, which take part in lipid oxidation, were altered. Their content in neoplastic tissue increased along with a worse clinical prognosis, specifically in lesions with a higher degree of malignancy and in samples without mutations in the IDH1/2 gene. It was also reported that SPME probes could be a helpful method for probing brain during neurosurgical procedures from two brain structures in replicates simultaneously. As a result, extracting a set of metabolites with a wide range of physicochemical properties was possible. Most of them were lipids with interesting patterns in studied structures. However, due to the small cohort of patients and high diversity of samples, a detailed discussion on the biological role and implications to the spatial distribution was not conducted. To sum up, solid-phase microextraction probes were useful in brain tumors lipidomic and acylcarnitine profiling of meningiomas and gliomas, considering malignancy degree and genotype. It has also been observed that chemical biopsy can serve as a valuable tool in in vivo brain research, indicating future directions for studies of brain tumors during neurosurgical procedures.

EN

Słowa kluczowe

PL

mikroekstrakcja do fazy stałej; guz mózgu; diagnostyka; lipidomika; spektrometria mas

EN

solid-phase microextraction; brain tumor; diagnostics; lipidomics; mass spectrometry

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Wiadomości Chemiczne
• Rocznik: 2025
• Tom: [Z] 79, 1-2
• Strony: 22--35
• Opis fizyczny: Bibliogr. 25 poz., rys., wykr.

Twórcy

autor

Bogusiewicz Joanna

• Katedra Farmakodynamiki i Farmakologii Molekularnej, Wydział Farmaceutyczny, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu

• https://orcid.org/0000-0003-4429-6854

autor

Burlikowska Katarzyna

• Katedra Farmakodynamiki i Farmakologii Molekularnej, Wydział Farmaceutyczny, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu

• https://orcid.org/0000-0001-8030-6226

autor

Kupcewicz Bogumiła

• Katedra Chemii Nieorganicznej i Analitycznej, Wydział Farmaceutyczny, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu

• https://orcid.org/0000-0002-4480-7338

autor

Łuczykowski Kamil

• Katedra Farmakodynamiki i Farmakologii Molekularnej, Wydział Farmaceutyczny, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu

• https://orcid.org/0000-0001-7254-0864

autor

Jaroch Karol

• Katedra Farmakodynamiki i Farmakologii Molekularnej, Wydział Farmaceutyczny, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu

• https://orcid.org/0000-0003-0189-2509

autor

Goryńska Paulina Zofia

• Katedra Farmakodynamiki i Farmakologii Molekularnej, Wydział Farmaceutyczny, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu

autor

Goryński Krzysztof

• Katedra Farmakodynamiki i Farmakologii Molekularnej, Wydział Farmaceutyczny, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu

autor

Birski Marcin

• Klinika Neurochirurgii, 10 Wojskowy Szpital Kliniczny z Polikliniką w Bydgoszczy

• https://orcid.org/0000-0001-6011-7559

autor

Furtak Jacek

• Klinika Neurochirurgii, 10 Wojskowy Szpital Kliniczny z Polikliniką w Bydgoszczy
• Wydział Medyczny, Politechnika Bydgoska im. Jana i Jędrzeja Śniadeckich

• https://orcid.org/0000-0001-9599-4156

autor

Paczkowski Dariusz

• Klinika Neurochirurgii, 10 Wojskowy Szpital Kliniczny z Polikliniką w Bydgoszczy
• Klinika Neurochirurgii i Neurologii, Szpital Uniwersytecki nr 2 im dr. Jana Biziela w Bydgoszczy

autor

Harat Marek

• Klinika Neurochirurgii, 10 Wojskowy Szpital Kliniczny z Polikliniką w Bydgoszczy
• Wydział Medyczny, Politechnika Bydgoska im. Jana i Jędrzeja Śniadeckich

• https://orcid.org/0000-0002-4043-2567

autor

Pawliszyn Janusz

• Wydział Chemii, Uniwersytet w Waterloo, Ontario, Kanada

autor

Bojko Barbara

• Katedra Farmakodynamiki i Farmakologii Molekularnej, Wydział Farmaceutyczny, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu

• https://orcid.org/0000-0003-3971-9816

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr POPUL/SP/0154/2024/02 w ramach programu "Społeczna odpowiedzialność nauki II" - moduł: Popularyzacja nauki (2025).