Stability-indicating RP-HPLC method for simultaneous determination of metformin hydrochloride and vildagliptin in tablet and biological samples

• Autorzy: Shakoor Abdul , Ahmed Mahmood , Ikram Rabia , Hussain Sajad , Tahir Arifa , Jan Badrul Mohamed , Adnan Ahmad

Identyfikatory

DOI

10.1556/1326.2019.00555

• Języki publikacji: EN

Abstrakty

EN

The present work aimed to develop and validate a simple, rapid, sensitive, accurate, and precise method for simultaneous determination of metformin hydrochloride and vildagliptin in tablet and biological samples. Isocratic elution of both the analytes was performed at 35 °C by injecting 20 μL into Thermo Hypersil ODS C18 column (5 μm, 4.6 mm× 250 mm), while the flow rate was set to 0.8 mL/min. The mobile phase comprised of methanol, acetonitrile, and phosphate buffer (5:30:65, v/v, pH 3.5), and wavelength was selected at 212 nm. The overall run time per sample was 7.0 min with a retention time of 3.36 and 5.41 min for metformin hydrochloride and vildagliptin, respectively. The calibration curve was linear from 10–140 μg/mL for metformin and 1–14 μg/mL for vildagliptin with a coefficient of determination (R2) ≤ 0.9919, while repeatability and reproducibility (expressed as relative standard deviation) were lower than 1.13 and 0.97%, respectively. Force degradation studies indicated a complete separation of the analytes in the presence of their degradation products providing a high degree of method specificity. The proposed reversed-phase high-performance liquid chromatography (RP-HPLC) method was demonstrated to be simple and rapid for the determination of metformin hydrochloride and vildagliptin in commercially available tablet and biological samples providing recoveries ranged between 100.13–100.29%.

Słowa kluczowe

EN

diabetes mellitus; metformin; vildagliptin; HPLC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2020
• Tom: Vol. 32, no. 1
• Strony: 39--43
• Opis fizyczny: Bibliogr. 19 poz., rys., tab.

Twórcy

autor

Shakoor Abdul

• Government College University, Lahore, Pakistan

autor

Ahmed Mahmood

• Renacon Parma Limited, Lahore, Pakistan

autor

Ikram Rabia

• University of Malaya, Kuala Lumpur 50603, Malaysia

autor

Hussain Sajad

• Government College University, Lahore, Pakistan

autor

Tahir Arifa

• Lahore College for Women University, Lahore, Pakistan

autor

Jan Badrul Mohamed

• University of Malaya, Kuala Lumpur 50603, Malaysia

autor

Adnan Ahmad

• Government College University, Lahore, Pakistan

Bibliografia

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Uwagi

PL

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).