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Substrate influence on the structure of methanogenic Archaea communities during anaerobic digestion

Treść / Zawartość
Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
This study compares the diversity of methanogenic Archaeal communities that developed during biogas production in reactors fed with different substrates. Reactor I was fed with silages of maize and of alfalfa and timothy; and Reactor II was fed with these silages plus pig slurry and glycerol as co substrates. The Archaeal community structure was studied using polymerase chain reaction––denaturing gradient gel electrophoresis based on the 16S rRNA gene. In both reactors, Methanosphaerula palustris was most abundant, and species belonging to Methanolinea, Methanoculleus, and Methanotorris were present. Only Reactor I, where the ammonia concentration was lower, had species belonging to Methanospirillum and Methanosarcina. Thus, it appears that addition of pig slurry increased the ammonia concentration, which inhibited the growth of Methanospirillum and Methanosarcina.
Rocznik
Strony
41--47
Opis fizyczny
Bibliogr. 21 poz., rys., tab.
Twórcy
  • Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Słoneczna 45G, 10-719 Olsztyn, Poland; Phone: +48 89 523 4144; Fax: +48 89 523 4131
  • Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Poland
  • Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Poland
Bibliografia
  • APHA (American Public Health Association). 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. American Public Health Association. Washington, DC.
  • Bułkowska, K., T. Pokój, E. Klimiuk, Z.M. Gusiatin. 2012. Optimization of anaerobic digestion of a mixture of Zea mays and Miscanthus sacchariflorus silages with various pig manure dosages. Bioresource Technology 125: 208-216.
  • Cadillo-Quiroz, H., J.B. Yavitt, S.H. Zinder. 2009. Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland. International Journal of Systematic and Evolutionary Microbiology 59: 928-935.
  • Ciesielski, S., K. Bułkowska, D. Dabrowska, D. Kaczmarczyk, P. Kowal, J. Mozejko. 2013. Ribosomal Intergenic Spacer Analysis as a tool for monitoring methanogenic Archaea in anaerobic digester. Current Microbiology 67: 240-248.
  • Crable, B.R., C.M. Plugge, M.J. McInerney, A.J.M. Stams. 2011. Formate formation and formate conversion in biological fuels production. Enzyme Research 2011, Article ID 532536: 1-8.
  • Hijazi, O., S. Munro, B. Zerhusen, M. Effenberger. 2016. Review of life cycle assessment for biogas production in Europe. Renewable and Sustainable Energy Reviews 54: 1291-1300.
  • Jiang, B., S.N. Parshina, W. Doesburg, B.P. Lomans, J.M. Stams. 2005. Methanomethylovorans thermophila sp. nov., a thermophilic, methylotrophic methanogen from an anaerobic reactor fed with methanol. International Journal of Systematic and Evolutionary Microbiology 55: 2465-2470.
  • Kim, I., M. Hwang, N. Jang, S. Hyun, S. Lee. 2004. Effect of low pH on the activity of hydrogen utilizing methanogens in bio-hydrogen process. International Journal of Hydrogen Energy 29: 1133-1140.
  • Kim, W., K. Cho, S. Lee, S. Hwang. 2013. Comparison of methanogenic community structure and anaerobic process performance treating swine wastewater between pilot and optimized lab scale bioreactors. Bioresource Technology 145: 48-56.
  • Klocke. M., E. Nettmann, I. Bergmann, K. Mundt, K. Souidi, J. Mumme, B. Linke. 2008. Characterization of the methanogenic Archaea within two phase biogas reactor systems operated with plant biomass. Systematic and Applied Microbiology 31: 190-205.
  • Krause, L., N.N. Diaz, R.A. Edwards, K.H. Gartemann, H. Kromeke, H. Neuweger, A. Puhler, K.J. Runte, A. Schluter, J. Stoye, R. Szczepanowski, A. Tauch, A. Goesmann. 2008. Taxonomic composition and gene content of a methane producing microbial community isolated from a biogas reactor. Journal of Biotechnology 136: 91-101.
  • Kröber, M., T. Bekel, N. Diaz, A. Goesmann, S. Jaenicke, L. Krause, D. Miller, J. Runte, P. Viehover, A. Puhler, A. Schluter. 2009. Phylogenetic characterization of a biogas plant microbial community integrating clone library 16S-rDNA sequences and metagenome sequence data obtained by 454-pyrosequencing. Journal of Biotechnology 142: 38-49.
  • Kumar, S., K. Tamura, I.B. Jakobsen, M. Nei. 2001. MEGA2: Molecular Evolutionary Genetics Analysis software. Bioinformatics 17: 1244-1245.
  • Nei, M., W.H. Li. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America 76: 5269-5273.
  • Sakai, S., M. Ehara, I.C. Tseng, T. Yamaguchi, S.L. Bräuer, H. Cadillo-Quiroz, S.H. Zinder, H. Imachi. 2012. Methanolinea mesophila sp. nov., a hydrogenotrophic methanogen isolated from rice field soil, and proposal of the Archaeal family Methanoregulaceae fam. nov. within the order Methanomicrobiales. International Journal of Systematic and Evolutionary Microbiology 62: 1389-1395.
  • Thompson, J.D., D.G. Higgins, T.J. Gibson. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions specific penalties and weight matrix choice. Nucleic Acids Research 22: 4673-4680.
  • van Hannen, E.J., W. Mooij, M.P. van Agterveld, H.J. Gons, H.J. Laanbroek. 1999. Detritus-dependent development of the microbial community in an experimental system: qualitative analysis by denaturing gradient gel electrophoresis. Applied and Environmental Microbiology 65: 2478-2484.
  • Vavilin, V.A., X. Qu, L. Mazéas, M. Lemunier, C. Duquennoi, P. He, T. Bouchez. 2008. Methanosarcina as the dominant aceticlastic methanogens during mesophilic anaerobic digestion of putrescible waste. Antonie Van Leeuwenhoek 94: 593-605.
  • Watanabe, T., S. Asakawa, A. Nakamura, Z. Nagaoka, M. Kimura. 2004. DGGE method for analyzing 16S rDNA of methanogenic archaeal community in paddy field soil. FEMS Microbiology Letters 232: 153-163.
  • Yenigün, O., B. Demirel. 2013. Ammonia inhibition in anaerobic digestion: A review. Process Biochemistry 48: 901-911.
  • Ziganshin, A.M., J. Liebetrau, J. Pröter, S. Kleinstenber. 2013. Microbial community structure and dynamics during anaerobic digestion of various agricultural waste materials. Applied Microbiology and Biotechnology 97: 5161-5174.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-86851251-1519-46e8-ad1b-b5b203a0a03e
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