Evaluating the suitability of plant and crustacean microbiotests for assessing soil toxicity caused by ethylbenzene contamination

• Autorzy: Kusza Grzegorz , Czerniawska-Kusza Izabela , Łuczak Katarzyna , Ciesielczuk Tomasz , Cichoń Aleksandra , Pisarek Izabella

Identyfikatory

DOI

10.24425/aep.2025.156013

Warianty tytułu

PL

Oszacowanie przydatności mikrobiotestów z roślinami i skorupiakami w ocenie toksyczności gleby spowodowanej zanieczyszczeniem etylobenzenem

• Języki publikacji: EN

Abstrakty

EN

Plant and crustacean microbiotests were assessed for their suitability in evaluating soil and groundwater contamination with ethylbenzene (EB) following a railway accident. Bioassays using Lepidium sativum, Sinapis alba, Sorghum saccharatum, Heterocypris incongruens, and Thamnocephalus platyurus were conducted to measure acute toxicity in both naturally and artificially contaminated podzolic soils. Results of direct contact tests showed significant correlations between toxicity endpoints and EB concentrations. In naturally contaminated soils (EB: 67–2865 mg·kg^-1), seed germination decreased by 17–52%, and root growth by 55–70%. L. sativum and H. incongruens exhibited the highest sensitivity. T. platyurus also responded to EB in soil pore water and groundwater, although only temporary narcotic effects were observed at lower concentrations (≤76 mg·dm^-3). In contrast, artificially spiked soils did not affect seed germination but inhibited root elongation and crustacean growth. These findings highlight the influence of environmental factors, such as contamination duration and soil moisture, on EB toxicity and support the application of microbiotests in evaluating contaminated soils.

PL

Oceniono przydatność mikrobiotestów roślinnych i ze skorupiakami w ocenie skażenia gleb i wód gruntowych etylobenzenem (EB) po katastrofie kolejowej. Przeprowadzono analizy z użyciem biotestów zawierających Lepidium sativum, Sinapis alba, Sorghum saccharatum, Heterocypris incongruens i Thamnocephalus platyurus w celu określenia toksyczności ostrej w glebach bielicowych zanieczyszczonych w środowisku naturalnym oraz w warunkach laboratoryjnych. Wyniki testów bezpośredniego kontaktu wykazały istotne korelacje pomiędzy efektami końcowymi a stężeniami EB. W glebach zanieczyszczonych (EB 67–2865 mg·kg^-1) odnotowano spadek kiełkowania nasion o 17-52% i zahamowanie przyrostu korzeni o 55-70%. L. sativum i H. incongruens wykazały najwyższą wrażliwość. T. platyurus również zareagował negatywnie na EB w wodzie porowej gleby i wodach gruntowych, choć przy niższych stężeniach (≤76 mg·dm^-3) obserwowano jedynie tym czasowe efekty narkotyczne. Natomiast gleby zanieczyszczone EB w warunkach laboratoryjnych nie wpłynęły na kiełkowanie nasion, ale hamowały wzrost korzeni oraz rozwój skorupiaków. Uzyskane wyniki podkreślają wpływ czynników środowiskowych, takich jak, czas trwania skażenia i wilgotność gleby, na toksyczność EB i wskazują na przydatność stosowania mikrobiotestów w ocenie zanieczyszczonych gleb.

Słowa kluczowe

EN

ethylbenzene; soil toxicity; phytotoxicity; microbiotests; bioassays

PL

etylobenzen; mikrobiotesty; toksyczność gleb; badania biologiczne; fitotoksyczność

• Wydawca: Institute of Environmental Engineering, Polish Academy of Sciences
• Czasopismo: Archives of Environmental Protection
• Rocznik: 2025
• Tom: Vol. 51, no. 3
• Strony: 111--119
• Opis fizyczny: Bibliogr. 44 poz., tab., wykr.

Twórcy

autor

Kusza Grzegorz

• Institute of Environmental Engineering and Biotechnology, University of Opole, Poland

• https://orcid.org/0000-0003-4329-8700

autor

Czerniawska-Kusza Izabela

• Institute of Biology, University of Opole, Poland

• https://orcid.org/0000-0003-0975-8152

autor

Łuczak Katarzyna

• Institute of Environmental Engineering and Biotechnology, University of Opole, Poland

• https://orcid.org/0000-0002-5777-9938

autor

Ciesielczuk Tomasz

• Institute of Environmental Engineering and Biotechnology, University of Opole, Poland

• https://orcid.org/0000-0003-4381-8091

autor

Cichoń Aleksandra

• Institute of Environmental Engineering and Biotechnology, University of Opole, Poland

• https://orcid.org/0000-0002-3709-7411

autor

Pisarek Izabella

• Institute of Environmental Engineering and Biotechnology, University of Opole, Poland

• https://orcid.org/0000-0002-5154-0846

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr POPUL/SP/0154/2024/02 w ramach programu "Społeczna odpowiedzialność nauki II" - moduł: Popularyzacja nauki (2025).