Environmental Application of Reporter-Genes Based Biosensors for Chemical Contamination Screening

• Autorzy: Matejczyk M. , Rosochacki S. J.

Identyfikatory

DOI

10.2478/aep-2014-0043

Warianty tytułu

PL

Zastosowanie opartych na genach reporterowych biosensorów w screeningu chemicznych zanieczyszczeń w środowisku

• Języki publikacji: EN

Abstrakty

EN

The paper presents results of research concerning possibilities of applications of reporter-genes based microorganisms, including the selective presentation of defects and advantages of different new scientific achievements of methodical solutions in genetic system constructions of biosensing elements for environmental research. The most robust and popular genetic fusion and new trends in reporter genes technology – such as LacZ (β-galactosidase), xylE (catechol 2,3-dioxygenase), gfp (green fluorescent proteins) and its mutated forms, lux (prokaryotic luciferase), luc (eukaryotic luciferase), phoA (alkaline phosphatase), gusA and gurA (β-glucuronidase), antibiotics and heavy metals resistance are described. Reporter-genes based biosensors with use of genetically modified bacteria and yeast successfully work for genotoxicity, bioavailability and oxidative stress assessment for detection and monitoring of toxic compounds in drinking water and different environmental samples, surface water, soil, sediments.

PL

Prezentowana praca przeglądowa zawiera opis możliwości aplikacyjnych biosensorów opartych na genetycznie zmodyfikowanych mikroorganizmach wyposażonych w geny reporterowe. W pracy przedstawiono defekty i zalety nowych naukowych osiągnięć oraz metodologicznych rozwiązań dotyczących genetycznych systemów w biosensorach przeznaczonych do środowiskowego screeningu zanieczyszczeń. Opisano najbardziej użyteczne i popularne genetyczne fuzje sekwencji promotorowych z takimi genami reporterowymi jak: lacZ (β-galaktozydaza), xylE (katechol 2,3-dioxygenaza), gfp (gen białka zielonej fluorescencji) oraz jego zmutowane warianty, lux (prokariotyczna lucyferaza), luc (eukariotyczna lucyferaza), phoA (alkaliczna fosfataza), gusA i gurA (β-glukuronidaza), geny oporności na antybiotyki oraz metale ciężkie. Tego typu mikrobiologiczne biosensory znalazły szerokie zastosowanie w testach genotoksyczności, badaniach nad biodostępnością oraz stresem oksydacyjnym, a także w detekcji i monitoringu substancji toksycznych w wodzie pitnej, różnych próbach środowiskowych, wodach powierzchniowych, glebie i osadach.

Słowa kluczowe

EN

reporter genes; biosensors; chemical contamination

PL

geny reporterowe; biosensor; skażenie chemiczne

• Wydawca: Institute of Environmental Engineering, Polish Academy of Sciences
• Czasopismo: Archives of Environmental Protection
• Rocznik: 2014
• Tom: Vol. 40, no. 4
• Strony: 113--123
• Opis fizyczny: Bibliogr. 50 poz., tab.

Twórcy

autor

Matejczyk M.

• Bialystok University of Technology, Faculty of Civil and Environmental Engineering, Division of Sanitary Biology and Biotechnology, ul. Wiejska 45 E, 15-351 Bialystok, Poland

autor

Rosochacki S. J.

• Bialystok University of Technology, Faculty of Civil and Environmental Engineering, Division of Sanitary Biology and Biotechnology, ul. Wiejska 45 E, 15-351 Bialystok, Poland

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