Deuterium isotope effects in mechanistic studies of biotransformations of L-tyrosine and p-hydroxyphenylpyruvic acid catalyzed by the enzyme L-phenylalanine dehydrogenase

• Autorzy: Pałka Katarzyna , Podsadni Katarzyna , Szymańska-Majchrzak Jolanta , Winnicka Elżbieta

Identyfikatory

DOI

10.2478/nuka-2025-0006

• Języki publikacji: EN

Abstrakty

EN

The mechanisms of the reversible oxidative deamination of L-tyrosine to p-hydroxyphenylpyruvic acid and reductive amination of phenylpyruvic acid to L-phenylalanine, both catalyzed by the enzyme L-phenylalanine dehydrogenase (PheDH, EC 1.4.1.20), were investigated using the kinetic isotope effect (KIE) and solvent isotope effect (SIE) methods. The values of deuterium kinetic effects in the 2-position of L-tyrosine and KIE in the (3S)-position of phenylpyruvic acid and solvent isotope effects for both reactions were determined using the non- -competitive spectrophotometric method. Some mechanistic details of these biotransformations were discussed.

Słowa kluczowe

EN

kinetic isotope effects; L-phenylalanine; phenylalanine dehydrogenase; p-hydroxyphenylpyruvic acid; solvent isotope effect; tyrosine

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2025
• Tom: Vol. 70, No. 2
• Strony: 51--56
• Opis fizyczny: Bibliogr. 25 poz., rys.

Twórcy

autor

Pałka Katarzyna

• Department of Chemistry University of Warsaw Pasteura St. 1, 02-093 Warsaw, Poland

• https://orcid.org/0000-0002-1798-6043

autor

Podsadni Katarzyna

• Department of Biochemistry, Medical Faculty, Medical University of Warsaw Żwirki i Wigury St. 61, 02-091 Warsaw, Poland

autor

Szymańska-Majchrzak Jolanta

• Department of Biochemistry, Medical Faculty, Medical University of Warsaw Żwirki i Wigury St. 61, 02-091 Warsaw, Poland

autor

Winnicka Elżbieta

• Department of Chemistry University of Warsaw Pasteura St. 1, 02-093 Warsaw, Poland

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr POPUL/SP/0154/2024/02 w ramach programu "Społeczna odpowiedzialność nauki II" - moduł: Popularyzacja nauki (2025).