Endothelial cells on pet vascular prostheses impregnated with polyester-based copolymers and coated with cell-adhesive protein assemblies

• Autorzy: Chlupac J. , Filova E. , Riedel T. , Brynda E. , Pamuła E. , Lisa V. , Bacakova L.
• Języki publikacji: EN

Abstrakty

EN

Arterial bypass surgery with synthetic vascular prostheses achieves poor patency rates compared to autogenous natural materials, and this is a challenge for tissue engineering research concerning small caliber vascular grafts. Modifications of the prosthetic surface followed by endothelial cell seeding may reduce thrombogenicity and intimal hyperplasia. Planar polyethylene terephthalate (PET) vascular prosthetic samples were impregnated with the copolymer poly(glycolide-L-lactide) (PGL) or with the terpolymer poly(glycolide-L-lactide-(e)caprolactone) (PGLCap) in order to lower the permeability of the knitted fabrics and ensure a less adhesive background. Subsequent modification with adhesive protein assemblies composed of collagen type I (Co) in conjunction with laminin (LM), fibronectin (FN) or fibrin (Fb) gel was performed to enhance cell adhesion. Bovine pulmonary artery endothelial cells (EC) of the CPAE line were seeded on to the coatings and subjected to static tissue culture conditions for 7 days. Impregnation of the PET prostheses decreased the initial adhesion and proliferation of the EC. After coating with the protein assemblies, the impregnated PET provided better substrates for cell culture than the protein-coated PET, on which the EC population started decreasing after 4 days of culture. The cells proliferated better on the CoFN, CoFb and CoFbFN coatings than on the Co and CoLM coatings. Impregnation type and adhesive matrix protein deposition may play an important role in successful endothelialization, healing and clinical performance of vascular grafts.

Słowa kluczowe

EN

vascular prostheses; polyethylene terephtalate; poly(glycolide-L-lactide); poly(glycolide-L-lactide-(ε)caprolactone); extracellular matrix; surface modification; collagen; laminin; fibronectin; fibrin; endothelial cells; static cell culture

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2008
• Tom: Vol. 11, no. 81-84
• Strony: 108--111
• Opis fizyczny: Bibliogr. 17 poz., tab., wykr., zdj.

Twórcy

autor

Chlupac J.

• Department of Growth and Differentiation of Cell Populations, Institute oF Physiology, Academy of Sciences of the Czech Republic, v.v.i., 1083 Videnska St., 142 20 Prague 4-Krc, Czech Republic
• Centre for Experimental Cardiovascular Research, Videnska St. 1083, 142 20 Prague 4-Krc, Czech Republic
• Trasnplant Surgery Clinic, Institute for Clinical and Experimental Medicine, 1958/9 Videnska st., 140 21 Prague 4-Krc, Czech Republic

autor

Filova E.

• Department of Growth and Differentiation of Cell Populations, Institute oF Physiology, Academy of Sciences of the Czech Republic, v.v.i., 1083 Videnska St., 142 20 Prague 4-Krc, Czech Republic
• Centre for Experimental Cardiovascular Research, Videnska St. 1083, 142 20 Prague 4-Krc, Czech Republic

autor

Riedel T.

• Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., 2 Heyerovsky Sq., 162 06 Prague-6 Petriny, Czech Republic

autor

Brynda E.

• Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., 2 Heyerovsky Sq., 162 06 Prague-6 Petriny, Czech Republic

autor

Pamuła E.

• Department of Biomaterials, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30 Mickiewicza Av., 30-059 Cracow, Poland

autor

Lisa V.

• Department of Growth and Differentiation of Cell Populations, Institute oF Physiology, Academy of Sciences of the Czech Republic, v.v.i., 1083 Videnska St., 142 20 Prague 4-Krc, Czech Republic

autor

Bacakova L.

• Department of Growth and Differentiation of Cell Populations, Institute oF Physiology, Academy of Sciences of the Czech Republic, v.v.i., 1083 Videnska St., 142 20 Prague 4-Krc, Czech Republic
• Centre for Experimental Cardiovascular Research, Videnska St. 1083, 142 20 Prague 4-Krc, Czech Republic

Bibliografia

• [1] Kannan, R. Y., et al., Current status of prosthetic bypass grafts: A review. Journal of Biomedical Materials Research, Part B: Applied Biomaterials, 2005, 74B(1): p. 570-581.
• [2] Xue, L. and H. P. Greisler, Biomaterials in the development and future ofvascular grafts. Journal of Vascular Surgery, 2003, 37(2): p. 472-480.
• [3] Chakfe, N., et al., Impregnated Polyester Arterial Prostheses: Performance and Prospects. Annals of Vascular Surgery, 1999, 13(5): p. 509-523.
• [4] Stegemann, J. P., H. Hong, and R. M. Nerem, Mechanical, biochemical, and extracellular matrix effects on vascular smooth muscle cell phenotype. Journal of Applied Physiology, 2005, 98(6): p. 2321 -2327.
• [5] Bacakova L., et al., Cell adhesion on artificial materials for tissue engineering. Physiological Research, 2004, 53 (Suppl 1): p. 35-45.
• [6] Bordenave, L., et al., In vitro endothelialized ePTFE prostheses: Clinical update 20 years after the first realization. Clinical Hemorheology and Microcirculation, 2005, 33(3): p. 227-234.
• [7] Joana, V., et al., Regulation of cell adhesion. Clinical Hemorheology and Microcirculation, 2005, 33(3): p. 167-188.
• [8] Meinhart, J. G., et al., Clinical autologous in vitro endothelialization of 153 infrainguinal ePTFE grafts. Annual Thoracic Surgery, 2001, 71(90050): p. S327-331.
• [9] Wang, X., et al., Development of Small-Diameter Vascular Grafts, World Journal of Surgery, 2007. 31(4): p. 682-689.
• [10] Muto, A., et al., Smooth muscle cell signal transduction: Implications of vascular biology for vascular surgeons. Journal of Vascular Surgery, 2007, 45(6, Supplement 1): p. A15-A24.
• [11] Vara, D.S., et al., Cardiovascular tissue engineering: state of the art. Pathol Biol (Paris), 2005. 53(10): p. 599-612.
• [12] Tiwari, A., et al., Improving the patency of vascular bypass grafts: the role of suture materials and surgical techniques on reducing anastomotic compliance mismatch. European Journal of Vascular and Endovascular Surgery, 2003, 25(4): p. 287-95.
• [13] Brynda, E., et al., Surface Immobilized Protein Multilayers for Cell Seeding. Langmuir, 2005. 21(17): p. 7877-7883.
• [14] Pamula, E., et al., Hydrolytic degradation of porous scaffolds for tissue engineering from terpolymer of l-lactide, [epsilon]-caprolactone and glycolide. Journal of Molecular Structure, 2005, 744-747: p. 557-562.
• [15] Dobrzynski, P., et al., Synthesis of biodegradable copolymers with the use of low toxic zirconium compounds. 1. Copolymerization of glycolide with L-lactide initiated by Zr(Acac)4. Macromolecules, 2001, 34(15): p. 5090-5098.
• [16] Zilla, P., D. Bezuidenhout, and P. Human, Prosthetic vascular grafts: wrong models, wrong questions and no healing. Biomaterials, 2007, 28(34): p. 5009-27.
• [17] Nerem, R.M. and A.E. Ensley, The tissue engineering of blood vessels and the heart. American Journal of Transplantation, 2004, 4 Suppl 6: p. 36-42.

Uwagi

Funding: Centre for Experimental Cardiovascular Research, Prague, Czech Republic, Grant Agency of the Acad. Sci. CR (grants No: 1QS500110564 and IAA400500507); Polish Ministry of Science (grant No: 3T08D01928).