Development and validation of a sensitive LC-MS/MS method for determination of valacyclovir in human plasma: Application to a bioequivalence study

• Autorzy: Konda R. K. , Chandu B. R. , Challa B. R. , Chandrasekhar K. B.
• Języki publikacji: EN

Abstrakty

EN

A simple, sensitive, and highly specific method has been developed for determination of valacyclovir (VL) in human plasma. The analytical procedure involves a solid-phase extraction method using Valacyclovir-D8 (VLD8) as an internal standard. Chromatographic separation was carried out on a reversed phase Zorbax, SB C18, 4.6 × 75 mm, 3.5μm column. Valacyclovir and Valacyclovir-D8 were detected with proton adducts at m/z 325.2 → 152.0 and 333.3 → 152.0 in multiple reaction monitoring (MRM) positive mode. The method was linear over the concentration range of 0.5–700.0 ng mL ^-1. The limit of detection (LOD) and limit of quantification (LOQ) for valacyclovir were 0.2 pg mL ^-1 and 0.5 ng mL ^-1, respectively. The method was shown to be precise with the average within-run and between-run variations of 0.7 to 3.5% and 3.1 to 4.7%, respectively. The average within-run and between-run accuracies of the method throughout its linear range were 96.7 to 97.9 and 94.7 to 97.3%, respectively. The mean recoveries of valacyclovir and Valacyclovir-D8 from human plasma by the developed method were 99.17 ± 10.78% and 110.84 ± 8.74%, respectively. The method was successfully applied in bioequivalence study with 20 healthy male volunteers under fasting condition.

Słowa kluczowe

EN

valacyclovir; LC-MS/MS; solid-phase extraction; bioequivalence

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2013
• Tom: Vol. 25, no. 4
• Strony: 669--686
• Opis fizyczny: Bibliogr. 14 poz., rys., tab.

Twórcy

autor

Konda R. K.

• Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India
• Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India

autor

Chandu B. R.

• Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India

autor

Challa B. R.

• Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India

autor

Chandrasekhar K. B.

• Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India

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