Screening of active components as scavenger of radical from perennial fujimoto bean (Hyacinth Bean) whole herb by HPLC-MS coupled radical reaction

• Autorzy: Shi G. F. , Wang Z. J. , Wang G.Y. , Yao R. X. , Chen F. W. , Shi Z. R.
• Języki publikacji: EN

Abstrakty

EN

High-performance liquid chromatography-mass spectrometry (HPLC-MS) method coupled with radical reaction for screening active ingredients from perennial fujimoto bean whole herb was established. The active ingredients, present in perennial fujimoto bean whole herb, possess scavenging effects towards 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide, peroxy radical, and hydroxyl radical. The radical scavenging abilities of these active ingredients were evaluated based on the relative peak areas in the HPLC chromatogram. The results indicate that potent antioxidants are present in the anhydrous methanol extract of perennial fujimoto bean whole herb. Based on HPLC-MS analysis, it was found that the scavenging ability can be mostly attributed to the presence of three compounds: cyanidin-3-o-β-d-glucopyranoside, troxerutin, and rutin. The structures were identified based on the MS and nuclear magnetic resonance (NMR) data. Free radical scavenging activity decreased in the following order: troxerutin > rutin > cyanidin-3-o-β-d-glucopyranoside.

Słowa kluczowe

EN

fujimoto bean; HPLC-MS; antioxidant; radical scavenging capacity

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2016
• Tom: Vol. 28, no. 1
• Strony: 19--31
• Opis fizyczny: Bibliogr. 25 poz., rys., tab.

Twórcy

autor

Shi G. F.

• School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China

autor

Wang Z. J.

• chool of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China

autor

Wang G.Y.

• School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China

autor

Yao R. X.

• School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China

autor

Chen F. W.

• School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China

autor

Shi Z. R.

• College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 201400, China

Bibliografia

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Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.