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The impact of electromagnetic fields with frequency of 50 Hz on metabolic activity of cells in vitro

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PL
Oddziaływanie pola elektromagnetycznego o częstotliwości 50 Hz na aktywność metaboliczną komórek w warunkach in vitro
Języki publikacji
EN
Abstrakty
EN
The article presents the results of research on the influence of the sinusoidal electromagnetic field with a frequency of 50 Hz on the metabolic activity of two cell lines normal BJ and cancer 143b, with different exposure times (0.5 h, 1 h, 2 h and 3 h). It is shown the difference in its effect on cancer and physiological line. In order to analyze the viability there was performed the MTT test.
PL
W artykule zostały zaprezentowane wyniki badań wpływu sinusoidalnego pola elektromagnetycznego o częstotliwości 50 Hz na aktywność metaboliczną dwóch linii komórkowych - normalnej BJ i nowotworowej 143b przy różnych czasach ekspozycji (0.5 h, 1 h, 2 h i 3 h). Wykazano różnice w oddziaływaniu na linię fizjologiczną oraz nowotworową. W celu dokonania analizy żywotności wykonano test MTT.
Rocznik
Strony
161--164
Opis fizyczny
Bibliogr. 23 poz., fot., wykr.
Twórcy
  • University of Rzeszow, Faculty of Mathematics and Natural Sciences
autor
  • University of Rzeszow, Institute of Applied Biotechnology and Basic Sciences
  • University of Rzeszow, Institute of Applied Biotechnology and Basic Sciences
  • University of Rzeszow, Institute of Applied Biotechnology and Basic Sciences
Bibliografia
  • 1. Baldi I., Coureau G., Jaffre A., Gruber A., Ducamp S., Provost D., Lebailly P., Vital A., Loiseau H., Salamon R. (2011) : Occupational and residential exposure to electromagnetic fields and risk of brain tumors in adults: A case-control study in Gironde, France, International Journal of Cancer, 129 (6), pp. 1477-1484
  • 2. Feychting M, Forssen U, Floderus B (1997) Occupational and residential magnetic field exposure and leukemia and central nervous system tumors. Epidemiology 8:384–389
  • 3. Verkasalo PK, Pukkala E, Hongisto MY, Valjus JE, Järvinen PJ, Heikkilä KV, Koskenvuo M (1993) Risk of cancer in Finnish children living close to power lines. Br Med J 307:895–899
  • 4. Kalmijan A.J. (1978) Experimetal evidence of geomagnetic orientation in elasmobranch fishes. Pp. 347-353 in Animal Migration, Navigation and Homing, K, Schmidt-Konig, editor; and W.K. Keeton, editor, eds. New York: Springer Verlag.
  • 5. Feychting M., Ahlbom A., Kheifets L. (2005) EMF and health. Ann Rev Public Health 26: 165-189.
  • 6. Blank M. (2008) Protein and DNA reactions stimulated by electromagnetic fields. Electromagn Biol Med. 27(1):3-23.
  • 7. Koh E.K., Ryu B.K., Jeong D.Y., Bang I.S., Nam M.H., Chae K.S. (2008) A 60-Hz sinusoidal magnetic field induces apoptosis of prostate cancer cells through reactive oxygen species. Int J Radiat Biol. 84: 945-955.
  • 8. Poniedzialek B., Rzymski P., Nawrocka-Bogusz H., Jaroszyk F., Wiktorowicz K. (2013) The effect of electromagnetic field on reactive oxygen species production in human neutrophils in vitro. Electromagn Biol Med. 32(3):333-41.
  • 9. Pirozzoli M.C., Marino C., Lovisolo G.A., Laconi C., Mosiello L., Negroni A. (2003) Effect of 50 Hz electromagnetic field exposure on apoptosis and differentiation in a neuroblastoma cell line. Bioelectromagnetics. 24(7):510-6.
  • 10. Berg H., Gunther B., Hilger I., Radeva M., Traitcheva N., Wollweber L. (2010) Bioelectromagnetic field effects on cancer cells and mice tumors. Electromagn Biol Med. 29(4):132-43.
  • 11. Pang L., Traitcheva N., Gothe G., Gomez J.A.C., Berg H. (2002) ELF-electromagnetic fields inhibit the proliferation of human cancer cells and induce apoptosis. Electromagnetic Biology and Medicine. 21: 243-248.
  • 12. Seeliger C., Fallidorf K., Sachtleben J., van Griensven M. (2014) Low-freqiency pulsed electromagnetic fields significantly improve time of closure and proliferation of human tendon fibroblasts. Eur J Med Res. 5;19-37.
  • 13. Koziorowska A., Pasiud E., Fila M., Romerowicz-Misielak M. (2016) The impact of electromagnetic field at a frequency of 50Hz and a magnetic induction of 2.5 mT on viability of pineal cells in vitro. J Biol Regul Homeost Agents. 30(4) in press.
  • 14. Reiter R. (1994) Melatonin suppression by static and extremely low frequency electromagnetic fields: relationship to the reported increased incidence of cancer. Rev. Environ. Health 10. 171-186.
  • 15. Martiny K., Lunde M., Bech P. (2010) Transcranial low voltage pulsed electromagnetic fields in patients with treatmentresistant depression. Biol Psychiatry. 15;68(2):163-9.
  • 16. Wolf F.I., Torsello A., Tedesco B., Fasanella S., Boninsegna A., D'Ascenzo M., et al. (2005) 50 Hz extremely low frequency electromagnetic fields enhance cell prolieration and DNA damage: Possible involvement of redox mechanisms. Biochim Biophys Acta. 1743:120-129.
  • 17. Vijayalaxmi, Prihoda T.J. (2009) Genetic damage in mammalian somatic cells exposed to extremely low frequency electromagnetic fields: a meta-analysis of 97 publications (1990-2007). Int J Radiat Biol. 85:196-2013.
  • 18. Simko M. (2007) Cell type specific redox status is responsible for diverse electromagnetic field effects. Cur Med Chem. 14:1142-1152.
  • 19. Nie K., Henderson A. (2003) MAP kinase activation in cells exposed to a 60 Hz magnetic field. J Cell Biochem. 90: 1197- 1206.
  • 20. Thumm S., Loschinger M., Glock S., Hammerle H., Rodemann H.P. (1999) Induction of cAMP-dependent protein kinase A activity in human skin fibroblasts and rat osteoblasts by extremely low-frequency electromagnetic fields. Radiat Environ Biophys. 38: 195-1999.
  • 21. Schimmelpfeng J., Stein J.C., Dertinger H. (1995) Action of 50 Hz magnetic fields on cyclic AMP and intercellular communication in monolayers and spheroids of mammalian cells. Bioelectromagnetics. 16:381-386.
  • 22. Cui Y., Liu X., Yang T., Mei Y.A., Hu C. (2014) Exposure to extremaly low-frequency electromagnetic fields inhibits T-type calcium channels via AA/LTE4 signaling pathway. Cell Calcium. 55: 48-58.
  • 23. Buckner C.A., Buckner A.L., Koren S.A., Persinger M.A., Lafrenie R.M. (2015) Inhibition of cancer cell growth by exposure to a specific time-varying electromagnetic field invilves T-type calcium channels. PLoS One. 14;1(4)e0124136.
Uwagi
Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-82ae9257-5b32-47b0-aa20-ee77b739c6e8
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