Development and validation of a stability-indicating HPTLC method for analysis of nebivolol hydrochloride and hydrochlorothiazide in the bulk material and in pharmaceutical dosage forms

• Autorzy: Damle M. C. , Topagi K. S. , Bothara K. G.
• Języki publikacji: EN

Abstrakty

EN

An accurate, sensitive, rapid, and precise stability-indicating high-performance thin-layer chromatographic (HPTLC) method for analysis of nebivolol hydrochloride and hydrochlorothiazide as the bulk drug and in tablets has been developed and validated. Optimum separation was achieved on silica gel 60 F 254 plates with ethyl acetate-methanol-acetic acid 6.5:1:0.5 ( υ/υ ) as mobile phase. Detection and quantification were performed at 280 and 270 nm for nebivolol hydrochloride and hydrochlorothiazide, respectively. The drugs get resolved with R F 0.46 ± 0.02 and 0.78 ± 0.02 for nebivolol hydrochloride and hydrochlorothiazide, respectively. The drugs were subjected to hydrolysis under acidic, basic, and neutral conditions, oxidation, heat, and photolysis as stress conditions. Peaks of degradation products were observed when the drugs were subjected to oxidative stress. Acidic conditions were also found to affect the tablet sample substantially. The degradation products resulting from stress conditions did not interfere with the drug peak. The method can be used for stability testing of these drugs during stability studies.

Słowa kluczowe

EN

nebivolol hydrochloride; hydrochlorothiazide; HPTLC; stability-indicating method

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2010
• Tom: Vol. 22, no. 3
• Strony: 433--443
• Opis fizyczny: Bibliogr. 20 poz., rys., tab.

Twórcy

autor

Damle M. C.

• AISSMS College of Pharmacy Pharmaceutical Chemistry Department Kennedy Road, Near RTO Pune 411001 Maharashtra India

autor

Topagi K. S.

• AISSMS College of Pharmacy Pharmaceutical Chemistry Department Kennedy Road, Near RTO Pune 411001 Maharashtra India

autor

Bothara K. G.

• AISSMS College of Pharmacy Pharmaceutical Chemistry Department Kennedy Road, Near RTO Pune 411001 Maharashtra India

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